Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.

Glucosinolates are token stimuli in host selection of many crucifer specialist insects, but the underlying molecular basis for host selection in these insects remains enigmatic. Using a combination of behavioral, electrophysiological, and molecular methods, we investigate glucosinolate receptors in...

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Autores principales:	Jun Yang, Hao Guo, Nan-Ji Jiang, Rui Tang, Guo-Cheng Li, Ling-Qiao Huang, Joop J A van Loon, Chen-Zhu Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f890761dabfd441d9bbbec7dd8d404c9
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spelling	oai:doaj.org-article:f890761dabfd441d9bbbec7dd8d404c92021-12-02T20:02:45ZIdentification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.1553-73901553-740410.1371/journal.pgen.1009527https://doaj.org/article/f890761dabfd441d9bbbec7dd8d404c92021-07-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009527https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Glucosinolates are token stimuli in host selection of many crucifer specialist insects, but the underlying molecular basis for host selection in these insects remains enigmatic. Using a combination of behavioral, electrophysiological, and molecular methods, we investigate glucosinolate receptors in the cabbage butterfly Pieris rapae. Sinigrin, as a potent feeding stimulant, elicited activity in larval maxillary lateral sensilla styloconica, as well as in adult medial tarsal sensilla. Two P. rapae gustatory receptor genes PrapGr28 and PrapGr15 were identified with high expression in female tarsi, and the subsequent functional analyses showed that Xenopus oocytes only expressing PrapGr28 had specific responses to sinigrin; when ectopically expressed in Drosophila sugar sensing neurons, PrapGr28 conferred sinigrin sensitivity to these neurons. RNA interference experiments further showed that knockdown of PrapGr28 reduced the sensitivity of adult medial tarsal sensilla to sinigrin. Taken together, we conclude that PrapGr28 is a gustatory receptor tuned to sinigrin in P. rapae, which paves the way for revealing the molecular basis of the relationships between crucifer plants and their specialist insects.Jun YangHao GuoNan-Ji JiangRui TangGuo-Cheng LiLing-Qiao HuangJoop J A van LoonChen-Zhu WangPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 7, p e1009527 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Jun Yang Hao Guo Nan-Ji Jiang Rui Tang Guo-Cheng Li Ling-Qiao Huang Joop J A van Loon Chen-Zhu Wang Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.
description	Glucosinolates are token stimuli in host selection of many crucifer specialist insects, but the underlying molecular basis for host selection in these insects remains enigmatic. Using a combination of behavioral, electrophysiological, and molecular methods, we investigate glucosinolate receptors in the cabbage butterfly Pieris rapae. Sinigrin, as a potent feeding stimulant, elicited activity in larval maxillary lateral sensilla styloconica, as well as in adult medial tarsal sensilla. Two P. rapae gustatory receptor genes PrapGr28 and PrapGr15 were identified with high expression in female tarsi, and the subsequent functional analyses showed that Xenopus oocytes only expressing PrapGr28 had specific responses to sinigrin; when ectopically expressed in Drosophila sugar sensing neurons, PrapGr28 conferred sinigrin sensitivity to these neurons. RNA interference experiments further showed that knockdown of PrapGr28 reduced the sensitivity of adult medial tarsal sensilla to sinigrin. Taken together, we conclude that PrapGr28 is a gustatory receptor tuned to sinigrin in P. rapae, which paves the way for revealing the molecular basis of the relationships between crucifer plants and their specialist insects.
format	article
author	Jun Yang Hao Guo Nan-Ji Jiang Rui Tang Guo-Cheng Li Ling-Qiao Huang Joop J A van Loon Chen-Zhu Wang
author_facet	Jun Yang Hao Guo Nan-Ji Jiang Rui Tang Guo-Cheng Li Ling-Qiao Huang Joop J A van Loon Chen-Zhu Wang
author_sort	Jun Yang
title	Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.
title_short	Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.
title_full	Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.
title_fullStr	Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.
title_full_unstemmed	Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.
title_sort	identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly pieris rapae.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f890761dabfd441d9bbbec7dd8d404c9
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Identification of a gustatory receptor tuned to sinigrin in the cabbage butterfly Pieris rapae.

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