Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.

Heat shock proteins (Hsps) are stress-responsive molecular chaperones, which uphold proper protein folding in response to external and internal stresses. The Hsp100 gene family plays a substantial role in thermos-tolerance of plants. This study investigated evolutionary relationship and expression o...

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Autores principales:	Sanober Gul, Kausar Nawaz Shah, Rashid Mehmood Rana, Muhammad Azam Khan, Ahmed M El-Shehawi, Mona M Elseehy
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f89a3427b1264091a38ed18b63529fbe
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spelling	oai:doaj.org-article:f89a3427b1264091a38ed18b63529fbe2021-12-02T20:18:11ZPhylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.1932-620310.1371/journal.pone.0255847https://doaj.org/article/f89a3427b1264091a38ed18b63529fbe2021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255847https://doaj.org/toc/1932-6203Heat shock proteins (Hsps) are stress-responsive molecular chaperones, which uphold proper protein folding in response to external and internal stresses. The Hsp100 gene family plays a substantial role in thermos-tolerance of plants. This study investigated evolutionary relationship and expression of ClpB/Hsp100 gene family in tomato under heat stress. Six SlHsp100 genes were identified using bioinformatics tools. In silico sub-cellular localization indicated that of these 6 ClpB/Hsp100 members, 4 are found in chloroplast, 1 in mitochondria and 1 in the cytoplasm. For evolutionary study, 36 SlHsp100 genes were included in the phylogenetic tree showing a hierarchical clustering shared by the members of the kingdoms Plantae, Archaea, Chromista, Fungi and Bacteria. A total 4 pairs of orthologous and 5 pairs of paralogous genes were identified. Functional divergence between different Hsp100 clusters showed considerable functional homology. Thermo-tolerance measured in terms of cell viability, cell membrane stability and pollen viability indicated that it was paralleled by thermal resistance of Hsps. Reverse transcriptase polymerase chain reaction was used to analyze gene expression in leaves of five-week-old tomato seedlings following exposure to heat stress (45°C) and control (25°C). Chloroplastic LeHSP110/ClpB gene was upregulated in all tomato genotypes after exposure to heat stress highlighting the crucial role of this gene family in acquired thermo-tolerance.Sanober GulKausar Nawaz ShahRashid Mehmood RanaMuhammad Azam KhanAhmed M El-ShehawiMona M ElseehyPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255847 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sanober Gul Kausar Nawaz Shah Rashid Mehmood Rana Muhammad Azam Khan Ahmed M El-Shehawi Mona M Elseehy Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.
description	Heat shock proteins (Hsps) are stress-responsive molecular chaperones, which uphold proper protein folding in response to external and internal stresses. The Hsp100 gene family plays a substantial role in thermos-tolerance of plants. This study investigated evolutionary relationship and expression of ClpB/Hsp100 gene family in tomato under heat stress. Six SlHsp100 genes were identified using bioinformatics tools. In silico sub-cellular localization indicated that of these 6 ClpB/Hsp100 members, 4 are found in chloroplast, 1 in mitochondria and 1 in the cytoplasm. For evolutionary study, 36 SlHsp100 genes were included in the phylogenetic tree showing a hierarchical clustering shared by the members of the kingdoms Plantae, Archaea, Chromista, Fungi and Bacteria. A total 4 pairs of orthologous and 5 pairs of paralogous genes were identified. Functional divergence between different Hsp100 clusters showed considerable functional homology. Thermo-tolerance measured in terms of cell viability, cell membrane stability and pollen viability indicated that it was paralleled by thermal resistance of Hsps. Reverse transcriptase polymerase chain reaction was used to analyze gene expression in leaves of five-week-old tomato seedlings following exposure to heat stress (45°C) and control (25°C). Chloroplastic LeHSP110/ClpB gene was upregulated in all tomato genotypes after exposure to heat stress highlighting the crucial role of this gene family in acquired thermo-tolerance.
format	article
author	Sanober Gul Kausar Nawaz Shah Rashid Mehmood Rana Muhammad Azam Khan Ahmed M El-Shehawi Mona M Elseehy
author_facet	Sanober Gul Kausar Nawaz Shah Rashid Mehmood Rana Muhammad Azam Khan Ahmed M El-Shehawi Mona M Elseehy
author_sort	Sanober Gul
title	Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.
title_short	Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.
title_full	Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.
title_fullStr	Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.
title_full_unstemmed	Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.
title_sort	phylogenetic and expression dynamics of tomato clpb/hsp100 gene under heat stress.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f89a3427b1264091a38ed18b63529fbe
work_keys_str_mv	AT sanobergul phylogeneticandexpressiondynamicsoftomatoclpbhsp100geneunderheatstress AT kausarnawazshah phylogeneticandexpressiondynamicsoftomatoclpbhsp100geneunderheatstress AT rashidmehmoodrana phylogeneticandexpressiondynamicsoftomatoclpbhsp100geneunderheatstress AT muhammadazamkhan phylogeneticandexpressiondynamicsoftomatoclpbhsp100geneunderheatstress AT ahmedmelshehawi phylogeneticandexpressiondynamicsoftomatoclpbhsp100geneunderheatstress AT monamelseehy phylogeneticandexpressiondynamicsoftomatoclpbhsp100geneunderheatstress
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Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress.

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