The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity

Synaptic vesicle (SV) release probability (Pr) is a key presynaptic determinant of synaptic strength established by cell-intrinsic properties and further refined by plasticity. To characterize mechanisms that generate Pr heterogeneity between distinct neuronal populations, we examined glutamatergic...

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Autores principales:	Chad W Sauvola, Yulia Akbergenova, Karen L Cunningham, Nicole A Aponte-Santiago, J Troy Littleton
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	synapse snare complex neurotransmitter release synaptic plasticity Drosophila synaptic vesicle Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f89cb7ee2d04449ba8e1a63036a262a4
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spelling	oai:doaj.org-article:f89cb7ee2d04449ba8e1a63036a262a42021-11-24T15:06:30ZThe decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity10.7554/eLife.728412050-084Xe72841https://doaj.org/article/f89cb7ee2d04449ba8e1a63036a262a42021-10-01T00:00:00Zhttps://elifesciences.org/articles/72841https://doaj.org/toc/2050-084XSynaptic vesicle (SV) release probability (Pr) is a key presynaptic determinant of synaptic strength established by cell-intrinsic properties and further refined by plasticity. To characterize mechanisms that generate Pr heterogeneity between distinct neuronal populations, we examined glutamatergic tonic (Ib) and phasic (Is) motoneurons in Drosophila with stereotyped differences in Pr and synaptic plasticity. We found the decoy soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) Tomosyn is differentially expressed between these motoneuron subclasses and contributes to intrinsic differences in their synaptic output. Tomosyn expression enables tonic release in Ib motoneurons by reducing SNARE complex formation and suppressing Pr to generate decreased levels of SV fusion and enhanced resistance to synaptic fatigue. In contrast, phasic release dominates when Tomosyn expression is low, enabling high intrinsic Pr at Is terminals at the expense of sustained release and robust presynaptic potentiation. In addition, loss of Tomosyn disrupts the ability of tonic synapses to undergo presynaptic homeostatic potentiation.Chad W SauvolaYulia AkbergenovaKaren L CunninghamNicole A Aponte-SantiagoJ Troy LittletoneLife Sciences Publications Ltdarticlesynapsesnare complexneurotransmitter releasesynaptic plasticityDrosophilasynaptic vesicleMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	synapse snare complex neurotransmitter release synaptic plasticity Drosophila synaptic vesicle Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	synapse snare complex neurotransmitter release synaptic plasticity Drosophila synaptic vesicle Medicine R Science Q Biology (General) QH301-705.5 Chad W Sauvola Yulia Akbergenova Karen L Cunningham Nicole A Aponte-Santiago J Troy Littleton The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity
description	Synaptic vesicle (SV) release probability (Pr) is a key presynaptic determinant of synaptic strength established by cell-intrinsic properties and further refined by plasticity. To characterize mechanisms that generate Pr heterogeneity between distinct neuronal populations, we examined glutamatergic tonic (Ib) and phasic (Is) motoneurons in Drosophila with stereotyped differences in Pr and synaptic plasticity. We found the decoy soluble N-ethylmaleimide sensitive factor attachment protein receptor (SNARE) Tomosyn is differentially expressed between these motoneuron subclasses and contributes to intrinsic differences in their synaptic output. Tomosyn expression enables tonic release in Ib motoneurons by reducing SNARE complex formation and suppressing Pr to generate decreased levels of SV fusion and enhanced resistance to synaptic fatigue. In contrast, phasic release dominates when Tomosyn expression is low, enabling high intrinsic Pr at Is terminals at the expense of sustained release and robust presynaptic potentiation. In addition, loss of Tomosyn disrupts the ability of tonic synapses to undergo presynaptic homeostatic potentiation.
format	article
author	Chad W Sauvola Yulia Akbergenova Karen L Cunningham Nicole A Aponte-Santiago J Troy Littleton
author_facet	Chad W Sauvola Yulia Akbergenova Karen L Cunningham Nicole A Aponte-Santiago J Troy Littleton
author_sort	Chad W Sauvola
title	The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity
title_short	The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity
title_full	The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity
title_fullStr	The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity
title_full_unstemmed	The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity
title_sort	decoy snare tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/f89cb7ee2d04449ba8e1a63036a262a4
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The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity

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