Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)

Abstract Although species delimitation is often controversial, emerging DNA-based and classical morphology-based methods are rarely compared using large-scale samplings, even less in the case of widely distributed species that have distant, allopatric populations. In the current study, we examined s...

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Autores principales:	Vladislav Ivanov, Yuri Marusik, Julien Pétillon, Marko Mutanen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8a6f76a14f942ffb472f7f6e694f1a6
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spelling	oai:doaj.org-article:f8a6f76a14f942ffb472f7f6e694f1a62021-12-02T10:47:54ZRelevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)10.1038/s41598-021-81788-22045-2322https://doaj.org/article/f8a6f76a14f942ffb472f7f6e694f1a62021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-81788-2https://doaj.org/toc/2045-2322Abstract Although species delimitation is often controversial, emerging DNA-based and classical morphology-based methods are rarely compared using large-scale samplings, even less in the case of widely distributed species that have distant, allopatric populations. In the current study, we examined species boundaries within two wolf spider species of the genus Pardosa (Araneae, Lycosidae), P. riparia and P. palustris. Wolf spiders constitute an excellent model for testing the relevance of traditional vs. modern methods in species and population delimitation because several closely related species are distributed over cross-continental geographic ranges. Allopatric populations of the two Pardosa species were sampled across Europe to Far East Russia (latitudinal range > 150°) and several dozen individuals were studied using morphological characters (morphometry of three measures for both sexes, plus five in males only and two in females only), DNA barcoding (COI sequencing) and double-digest restriction site associated DNA sequencing (ddRADseq). The results obtained allow for changing the taxonomic status of two Far East Russian populations to subspecies and ddRADseq proved to be a powerful tool for taxonomic research despite scarce sampling and inherent subjectivity of species delimitation in allopatry. Overall, this study pleads for both multi-criteria and more population-based studies in taxonomy.Vladislav IvanovYuri MarusikJulien PétillonMarko MutanenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vladislav Ivanov Yuri Marusik Julien Pétillon Marko Mutanen Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)
description	Abstract Although species delimitation is often controversial, emerging DNA-based and classical morphology-based methods are rarely compared using large-scale samplings, even less in the case of widely distributed species that have distant, allopatric populations. In the current study, we examined species boundaries within two wolf spider species of the genus Pardosa (Araneae, Lycosidae), P. riparia and P. palustris. Wolf spiders constitute an excellent model for testing the relevance of traditional vs. modern methods in species and population delimitation because several closely related species are distributed over cross-continental geographic ranges. Allopatric populations of the two Pardosa species were sampled across Europe to Far East Russia (latitudinal range > 150°) and several dozen individuals were studied using morphological characters (morphometry of three measures for both sexes, plus five in males only and two in females only), DNA barcoding (COI sequencing) and double-digest restriction site associated DNA sequencing (ddRADseq). The results obtained allow for changing the taxonomic status of two Far East Russian populations to subspecies and ddRADseq proved to be a powerful tool for taxonomic research despite scarce sampling and inherent subjectivity of species delimitation in allopatry. Overall, this study pleads for both multi-criteria and more population-based studies in taxonomy.
format	article
author	Vladislav Ivanov Yuri Marusik Julien Pétillon Marko Mutanen
author_facet	Vladislav Ivanov Yuri Marusik Julien Pétillon Marko Mutanen
author_sort	Vladislav Ivanov
title	Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)
title_short	Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)
title_full	Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)
title_fullStr	Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)
title_full_unstemmed	Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)
title_sort	relevance of ddradseq method for species and population delimitation of closely related and widely distributed wolf spiders (araneae, lycosidae)
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f8a6f76a14f942ffb472f7f6e694f1a6
work_keys_str_mv	AT vladislavivanov relevanceofddradseqmethodforspeciesandpopulationdelimitationofcloselyrelatedandwidelydistributedwolfspidersaraneaelycosidae AT yurimarusik relevanceofddradseqmethodforspeciesandpopulationdelimitationofcloselyrelatedandwidelydistributedwolfspidersaraneaelycosidae AT julienpetillon relevanceofddradseqmethodforspeciesandpopulationdelimitationofcloselyrelatedandwidelydistributedwolfspidersaraneaelycosidae AT markomutanen relevanceofddradseqmethodforspeciesandpopulationdelimitationofcloselyrelatedandwidelydistributedwolfspidersaraneaelycosidae
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Relevance of ddRADseq method for species and population delimitation of closely related and widely distributed wolf spiders (Araneae, Lycosidae)

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