Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes

Abstract The purpose of the present study was to assess the differential intraocular pressure response (IOP) to dexamethasone (DEX) treatment at two dose levels (100 or 500 nM) in perfusion cultured Indian cadaveric eyes to investigate glucocorticoid (GC) responsiveness. In a human organ-cultured an...

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Autores principales:	Ravinarayanan Haribalaganesh, Chidambaranathan Gowri Priya, Rajendrababu Sharmila, Subbaiah Krishnadas, Veerappan Muthukkaruppan, Colin E. Willoughby, Srinivasan Senthilkumari
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8b2cc5fcabf4d74b09ab9a8936d925b
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spelling	oai:doaj.org-article:f8b2cc5fcabf4d74b09ab9a8936d925b2021-12-02T14:12:08ZAssessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes10.1038/s41598-020-80112-82045-2322https://doaj.org/article/f8b2cc5fcabf4d74b09ab9a8936d925b2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80112-8https://doaj.org/toc/2045-2322Abstract The purpose of the present study was to assess the differential intraocular pressure response (IOP) to dexamethasone (DEX) treatment at two dose levels (100 or 500 nM) in perfusion cultured Indian cadaveric eyes to investigate glucocorticoid (GC) responsiveness. In a human organ-cultured anterior segment (HOCAS) set-up, the eye pressure was monitored for every 24 h post DEX infusion (100 or 500 nM) or 0.1% ethanol treatment for 7 days after baseline stabilization. The expression of DEX-inducible proteins such as myocilin and fibronectin in HOCAS-TM tissues was assessed by immunostaining. Elevated IOP was observed in 6/16 eyes [Mean ± SEM (mΔIOP): 15.50 ± 1.96 mmHg; 37.5% responders] and 3/15 eyes (Mean ± SEM mΔIOP: 10 ± 0.84 mmHg; 20% responders) in 100 nM and 500 nM dose groups respectively. Elevated IOP in GC responder eyes was substantiated with a significant increase in myocilin (11.8-fold; p = 0.0002) and fibronectin (eightfold; p = 0.04) expression as compared to vehicle-treated eyes by immunofluorescence analysis. This is the first study reporting the GC responsiveness in Indian cadaveric eyes. The observed GC response rate was comparable with the previous studies and hence, this model will enable us to investigate the relationship between differential gene expression and individual GC responsiveness in our population.Ravinarayanan HaribalaganeshChidambaranathan Gowri PriyaRajendrababu SharmilaSubbaiah KrishnadasVeerappan MuthukkaruppanColin E. WilloughbySrinivasan SenthilkumariNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ravinarayanan Haribalaganesh Chidambaranathan Gowri Priya Rajendrababu Sharmila Subbaiah Krishnadas Veerappan Muthukkaruppan Colin E. Willoughby Srinivasan Senthilkumari Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes
description	Abstract The purpose of the present study was to assess the differential intraocular pressure response (IOP) to dexamethasone (DEX) treatment at two dose levels (100 or 500 nM) in perfusion cultured Indian cadaveric eyes to investigate glucocorticoid (GC) responsiveness. In a human organ-cultured anterior segment (HOCAS) set-up, the eye pressure was monitored for every 24 h post DEX infusion (100 or 500 nM) or 0.1% ethanol treatment for 7 days after baseline stabilization. The expression of DEX-inducible proteins such as myocilin and fibronectin in HOCAS-TM tissues was assessed by immunostaining. Elevated IOP was observed in 6/16 eyes [Mean ± SEM (mΔIOP): 15.50 ± 1.96 mmHg; 37.5% responders] and 3/15 eyes (Mean ± SEM mΔIOP: 10 ± 0.84 mmHg; 20% responders) in 100 nM and 500 nM dose groups respectively. Elevated IOP in GC responder eyes was substantiated with a significant increase in myocilin (11.8-fold; p = 0.0002) and fibronectin (eightfold; p = 0.04) expression as compared to vehicle-treated eyes by immunofluorescence analysis. This is the first study reporting the GC responsiveness in Indian cadaveric eyes. The observed GC response rate was comparable with the previous studies and hence, this model will enable us to investigate the relationship between differential gene expression and individual GC responsiveness in our population.
format	article
author	Ravinarayanan Haribalaganesh Chidambaranathan Gowri Priya Rajendrababu Sharmila Subbaiah Krishnadas Veerappan Muthukkaruppan Colin E. Willoughby Srinivasan Senthilkumari
author_facet	Ravinarayanan Haribalaganesh Chidambaranathan Gowri Priya Rajendrababu Sharmila Subbaiah Krishnadas Veerappan Muthukkaruppan Colin E. Willoughby Srinivasan Senthilkumari
author_sort	Ravinarayanan Haribalaganesh
title	Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes
title_short	Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes
title_full	Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes
title_fullStr	Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes
title_full_unstemmed	Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes
title_sort	assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured indian cadaveric eyes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f8b2cc5fcabf4d74b09ab9a8936d925b
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Assessment of differential intraocular pressure response to dexamethasone treatment in perfusion cultured Indian cadaveric eyes

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