Essential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>

In the worldwide context of an impending emergence of multidrug-resistant bacteria, this research combined the advantages of multiple lipid nanoparticles (MLNs) and the promising therapeutic use of essential oils (EOs) as a strategy to fight the antibiotic resistance of three <i>Pseudomonas ae...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Rayhane Ben-Khalifa, Frédéric Bustos Gaspar, Cristina Pereira, Leila Chekir-Ghedira, Soraya Rodríguez-Rojo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	multiple lipid nanoparticles rosemary essential oil cefepime encapsulation antimicrobial activity <i>Pseudomonas aeruginosa</i> Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/f8bafa5249504ac1b4639c09a58eeb7c
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f8bafa5249504ac1b4639c09a58eeb7c
record_format	dspace
spelling	oai:doaj.org-article:f8bafa5249504ac1b4639c09a58eeb7c2021-11-25T16:22:20ZEssential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>10.3390/antibiotics101113002079-6382https://doaj.org/article/f8bafa5249504ac1b4639c09a58eeb7c2021-10-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1300https://doaj.org/toc/2079-6382In the worldwide context of an impending emergence of multidrug-resistant bacteria, this research combined the advantages of multiple lipid nanoparticles (MLNs) and the promising therapeutic use of essential oils (EOs) as a strategy to fight the antibiotic resistance of three <i>Pseudomonas aeruginosa</i> strains with different cefepime (FEP) resistance profiles. MLNs were prepared by ultrasonication using glyceryl trioleate (GTO) and glyceryl tristearate (GTS) as a liquid and a solid lipid, respectively. Rosemary EO (REO) was selected as the model EO. REO/FEP-loaded MLNs were characterized by their small size (~110 nm), important encapsulation efficiency, and high physical stability over time (60 days). An assessment of the antimicrobial activity was performed using antimicrobial susceptibility testing assays against selected <i>P. aeruginosa</i> strains. The assays showed a considerable increase in the antibacterial property of REO-loaded MLNs compared with the effect of crude EO, especially against <i>P. aeruginosa</i> ATCC 9027, in which the minimum inhibitory concentration (MIC) value decreased from 80 to 0.6 mg/mL upon encapsulation. Furthermore, the incorporation of FEP in MLNs stabilized the drug without affecting its antipseudomonal activity. Thus, the ability to co-encapsulate an essential oil and a hydrophilic antibiotic into MLN has been successfully proved, opening new possibilities for the treatment of serious antimicrobial infections.Rayhane Ben-KhalifaFrédéric Bustos GasparCristina PereiraLeila Chekir-GhediraSoraya Rodríguez-RojoMDPI AGarticlemultiple lipid nanoparticlesrosemary essential oilcefepimeencapsulationantimicrobial activity<i>Pseudomonas aeruginosa</i>Therapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1300, p 1300 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	multiple lipid nanoparticles rosemary essential oil cefepime encapsulation antimicrobial activity <i>Pseudomonas aeruginosa</i> Therapeutics. Pharmacology RM1-950
spellingShingle	multiple lipid nanoparticles rosemary essential oil cefepime encapsulation antimicrobial activity <i>Pseudomonas aeruginosa</i> Therapeutics. Pharmacology RM1-950 Rayhane Ben-Khalifa Frédéric Bustos Gaspar Cristina Pereira Leila Chekir-Ghedira Soraya Rodríguez-Rojo Essential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>
description	In the worldwide context of an impending emergence of multidrug-resistant bacteria, this research combined the advantages of multiple lipid nanoparticles (MLNs) and the promising therapeutic use of essential oils (EOs) as a strategy to fight the antibiotic resistance of three <i>Pseudomonas aeruginosa</i> strains with different cefepime (FEP) resistance profiles. MLNs were prepared by ultrasonication using glyceryl trioleate (GTO) and glyceryl tristearate (GTS) as a liquid and a solid lipid, respectively. Rosemary EO (REO) was selected as the model EO. REO/FEP-loaded MLNs were characterized by their small size (~110 nm), important encapsulation efficiency, and high physical stability over time (60 days). An assessment of the antimicrobial activity was performed using antimicrobial susceptibility testing assays against selected <i>P. aeruginosa</i> strains. The assays showed a considerable increase in the antibacterial property of REO-loaded MLNs compared with the effect of crude EO, especially against <i>P. aeruginosa</i> ATCC 9027, in which the minimum inhibitory concentration (MIC) value decreased from 80 to 0.6 mg/mL upon encapsulation. Furthermore, the incorporation of FEP in MLNs stabilized the drug without affecting its antipseudomonal activity. Thus, the ability to co-encapsulate an essential oil and a hydrophilic antibiotic into MLN has been successfully proved, opening new possibilities for the treatment of serious antimicrobial infections.
format	article
author	Rayhane Ben-Khalifa Frédéric Bustos Gaspar Cristina Pereira Leila Chekir-Ghedira Soraya Rodríguez-Rojo
author_facet	Rayhane Ben-Khalifa Frédéric Bustos Gaspar Cristina Pereira Leila Chekir-Ghedira Soraya Rodríguez-Rojo
author_sort	Rayhane Ben-Khalifa
title	Essential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>
title_short	Essential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>
title_full	Essential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>
title_fullStr	Essential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>
title_full_unstemmed	Essential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>
title_sort	essential oil and hydrophilic antibiotic co-encapsulation in multiple lipid nanoparticles: proof of concept and in vitro activity against <i>pseudomonas aeruginosa</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f8bafa5249504ac1b4639c09a58eeb7c
work_keys_str_mv	AT rayhanebenkhalifa essentialoilandhydrophilicantibioticcoencapsulationinmultiplelipidnanoparticlesproofofconceptandinvitroactivityagainstipseudomonasaeruginosai AT fredericbustosgaspar essentialoilandhydrophilicantibioticcoencapsulationinmultiplelipidnanoparticlesproofofconceptandinvitroactivityagainstipseudomonasaeruginosai AT cristinapereira essentialoilandhydrophilicantibioticcoencapsulationinmultiplelipidnanoparticlesproofofconceptandinvitroactivityagainstipseudomonasaeruginosai AT leilachekirghedira essentialoilandhydrophilicantibioticcoencapsulationinmultiplelipidnanoparticlesproofofconceptandinvitroactivityagainstipseudomonasaeruginosai AT sorayarodriguezrojo essentialoilandhydrophilicantibioticcoencapsulationinmultiplelipidnanoparticlesproofofconceptandinvitroactivityagainstipseudomonasaeruginosai
_version_	1718413236499382272

Essential Oil and Hydrophilic Antibiotic Co-Encapsulation in Multiple Lipid Nanoparticles: Proof of Concept and In Vitro Activity against <i>Pseudomonas aeruginosa</i>

Ejemplares similares