Autologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect

Yu Wang, Hao Zhang, Min Zhou, Xinzeyu Yi, Ping Duan, Aixi Yu, Baiwen Qi Department of Orthopaedic Trauma and Microsurgery, Wuhan University Zhongnan Hospital, Wuhan, Hubei 430071, People’s Republic of ChinaCorrespondence: Aixi Yu; Baiwen QiWuhan University Zhongnan Hospital, 169 East Lake...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wang Y, Zhang H, Zhou M, Yi X, Duan P, Yu A, Qi B
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	diabetic skin defects autologous fat grafting wound healing macrophage depletion Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/f8bc78b71a704566b6d8f492d9c2ccbc
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f8bc78b71a704566b6d8f492d9c2ccbc
record_format	dspace
spelling	oai:doaj.org-article:f8bc78b71a704566b6d8f492d9c2ccbc2021-12-02T15:12:04ZAutologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect1178-7007https://doaj.org/article/f8bc78b71a704566b6d8f492d9c2ccbc2020-12-01T00:00:00Zhttps://www.dovepress.com/autologous-fat-grafting-promotes-macrophage-infiltration-to-increase-s-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Yu Wang, Hao Zhang, Min Zhou, Xinzeyu Yi, Ping Duan, Aixi Yu, Baiwen Qi Department of Orthopaedic Trauma and Microsurgery, Wuhan University Zhongnan Hospital, Wuhan, Hubei 430071, People’s Republic of ChinaCorrespondence: Aixi Yu; Baiwen QiWuhan University Zhongnan Hospital, 169 East Lake Road, Wuchang District, Wuhan, Hubei 430071, People’s Republic of ChinaTel/Fax +86 67813120Email yuaixi@whu.edu.cn; wb002162@whu.edu.cnBackground: Diabetic skin defect is difficult to manage in surgical clinics, and there is still lack of effective treatments for diabetic skin defects. Currently, autologous fat grafting (AFG) is promising in the field of reconstructive surgery, while macrophage infiltration in autologous adipose tissue is considered vital for tissue regeneration. But AFG is rarely applied to the treatment of diabetic skin defects, and whether macrophage infiltration assists AFG to promote wound healing is still unknown.Methods: Full-thickness skin defect diabetic rats were divided into 3 groups: control group, autologous fat grafting (AFG) group and AFG with macrophage depletion (AFG+MD) group. We examined the amount of macrophages in the wounds bed and the expression level of inflammatory factors IL-10, IL-6, TNF-α, and also growth factors PDGF-β, TGF-β, IGF-1 at the same time. The content of collagen-I and α-smooth muscle actin protein in the wounds were determined by Western blot analysis. Finally, the healing of the wounds was evaluated.Results: The AFG group showing more rapid healing, secreting more growth factors and more obvious vascularization in the healing process, compared with the control group. But, the secretion of growth factors and the construction of extracellular matrix (ECM) in the wounds were limited when macrophages were depleted after AFG.Conclusion: AFG promotes the infiltration of macrophages to improve the healing environment of diabetic wounds by increasing the secretion of growth factors and revascularization, which provides a potential method for the treatment of diabetic skin defects.Keywords: diabetic skin defects, autologous fat grafting, wound healing, macrophage depletionWang YZhang HZhou MYi XDuan PYu AQi BDove Medical Pressarticlediabetic skin defectsautologous fat graftingwound healingmacrophage depletionSpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 4897-4908 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	diabetic skin defects autologous fat grafting wound healing macrophage depletion Specialties of internal medicine RC581-951
spellingShingle	diabetic skin defects autologous fat grafting wound healing macrophage depletion Specialties of internal medicine RC581-951 Wang Y Zhang H Zhou M Yi X Duan P Yu A Qi B Autologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect
description	Yu Wang, Hao Zhang, Min Zhou, Xinzeyu Yi, Ping Duan, Aixi Yu, Baiwen Qi Department of Orthopaedic Trauma and Microsurgery, Wuhan University Zhongnan Hospital, Wuhan, Hubei 430071, People’s Republic of ChinaCorrespondence: Aixi Yu; Baiwen QiWuhan University Zhongnan Hospital, 169 East Lake Road, Wuchang District, Wuhan, Hubei 430071, People’s Republic of ChinaTel/Fax +86 67813120Email yuaixi@whu.edu.cn; wb002162@whu.edu.cnBackground: Diabetic skin defect is difficult to manage in surgical clinics, and there is still lack of effective treatments for diabetic skin defects. Currently, autologous fat grafting (AFG) is promising in the field of reconstructive surgery, while macrophage infiltration in autologous adipose tissue is considered vital for tissue regeneration. But AFG is rarely applied to the treatment of diabetic skin defects, and whether macrophage infiltration assists AFG to promote wound healing is still unknown.Methods: Full-thickness skin defect diabetic rats were divided into 3 groups: control group, autologous fat grafting (AFG) group and AFG with macrophage depletion (AFG+MD) group. We examined the amount of macrophages in the wounds bed and the expression level of inflammatory factors IL-10, IL-6, TNF-α, and also growth factors PDGF-β, TGF-β, IGF-1 at the same time. The content of collagen-I and α-smooth muscle actin protein in the wounds were determined by Western blot analysis. Finally, the healing of the wounds was evaluated.Results: The AFG group showing more rapid healing, secreting more growth factors and more obvious vascularization in the healing process, compared with the control group. But, the secretion of growth factors and the construction of extracellular matrix (ECM) in the wounds were limited when macrophages were depleted after AFG.Conclusion: AFG promotes the infiltration of macrophages to improve the healing environment of diabetic wounds by increasing the secretion of growth factors and revascularization, which provides a potential method for the treatment of diabetic skin defects.Keywords: diabetic skin defects, autologous fat grafting, wound healing, macrophage depletion
format	article
author	Wang Y Zhang H Zhou M Yi X Duan P Yu A Qi B
author_facet	Wang Y Zhang H Zhou M Yi X Duan P Yu A Qi B
author_sort	Wang Y
title	Autologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect
title_short	Autologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect
title_full	Autologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect
title_fullStr	Autologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect
title_full_unstemmed	Autologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect
title_sort	autologous fat grafting promotes macrophage infiltration to increase secretion of growth factors and revascularization, thereby treating diabetic rat skin defect
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/f8bc78b71a704566b6d8f492d9c2ccbc
work_keys_str_mv	AT wangy autologousfatgraftingpromotesmacrophageinfiltrationtoincreasesecretionofgrowthfactorsandrevascularizationtherebytreatingdiabeticratskindefect AT zhangh autologousfatgraftingpromotesmacrophageinfiltrationtoincreasesecretionofgrowthfactorsandrevascularizationtherebytreatingdiabeticratskindefect AT zhoum autologousfatgraftingpromotesmacrophageinfiltrationtoincreasesecretionofgrowthfactorsandrevascularizationtherebytreatingdiabeticratskindefect AT yix autologousfatgraftingpromotesmacrophageinfiltrationtoincreasesecretionofgrowthfactorsandrevascularizationtherebytreatingdiabeticratskindefect AT duanp autologousfatgraftingpromotesmacrophageinfiltrationtoincreasesecretionofgrowthfactorsandrevascularizationtherebytreatingdiabeticratskindefect AT yua autologousfatgraftingpromotesmacrophageinfiltrationtoincreasesecretionofgrowthfactorsandrevascularizationtherebytreatingdiabeticratskindefect AT qib autologousfatgraftingpromotesmacrophageinfiltrationtoincreasesecretionofgrowthfactorsandrevascularizationtherebytreatingdiabeticratskindefect
_version_	1718387668257079296

Autologous Fat Grafting Promotes Macrophage Infiltration to Increase Secretion of Growth Factors and Revascularization, Thereby Treating Diabetic Rat Skin Defect

Ejemplares similares