The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion.
Prosaposin (PSAP), a highly conserved glycoprotein, is a precursor of saposins A-D. Accumulating evidence suggests that PSAP is a neurotrophic factor, as well as a regulator of lysosomal enzymes. Recently, the orphan G-protein-coupled receptors GPR37 and GPR37L1 were recognized as PSAP receptors, bu...
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2021
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oai:doaj.org-article:f8c6f4a7e8654219b2bdbded91c774e12021-12-02T20:18:21ZThe expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion.1932-620310.1371/journal.pone.0255958https://doaj.org/article/f8c6f4a7e8654219b2bdbded91c774e12021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0255958https://doaj.org/toc/1932-6203Prosaposin (PSAP), a highly conserved glycoprotein, is a precursor of saposins A-D. Accumulating evidence suggests that PSAP is a neurotrophic factor, as well as a regulator of lysosomal enzymes. Recently, the orphan G-protein-coupled receptors GPR37 and GPR37L1 were recognized as PSAP receptors, but their functions have not yet been clarified. In this study, we examined the distribution of PSAP and its receptors in the dorsal root ganglion (DRG) during development using specific antibodies, and showed that PSAP accumulates primarily in lysosomes and is dispersed throughout the cytoplasm of satellite cells. Later, PSAP colocalized with two receptors in satellite cells, and formed a characteristic ring shape approximately 8 weeks after birth, during a period of rapid DRG development. This ring shape, which was only observed around larger neurons, is evidence that several satellite cells are synchronously activated. We found that sortilin, a transporter of a wide variety of intracellular proteins containing PSAP, is strongly localized to the inner side of satellite cells, which contact the neuronal surface. These findings suggest that PSAP and GPR37/GPR37L1 play a role in activating both satellite and nerve cells.Miho TaniguchiHiroaki NabekaKimiko YamamiyaMd Sakirul Islam KhanTetsuya ShimokawaFarzana IslamTakuya DoiharaHiroyuki WakisakaNaoto KobayashiFumihiko HamadaSeiji MatsudaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 8, p e0255958 (2021) |
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Medicine R Science Q |
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Medicine R Science Q Miho Taniguchi Hiroaki Nabeka Kimiko Yamamiya Md Sakirul Islam Khan Tetsuya Shimokawa Farzana Islam Takuya Doihara Hiroyuki Wakisaka Naoto Kobayashi Fumihiko Hamada Seiji Matsuda The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion. |
| description |
Prosaposin (PSAP), a highly conserved glycoprotein, is a precursor of saposins A-D. Accumulating evidence suggests that PSAP is a neurotrophic factor, as well as a regulator of lysosomal enzymes. Recently, the orphan G-protein-coupled receptors GPR37 and GPR37L1 were recognized as PSAP receptors, but their functions have not yet been clarified. In this study, we examined the distribution of PSAP and its receptors in the dorsal root ganglion (DRG) during development using specific antibodies, and showed that PSAP accumulates primarily in lysosomes and is dispersed throughout the cytoplasm of satellite cells. Later, PSAP colocalized with two receptors in satellite cells, and formed a characteristic ring shape approximately 8 weeks after birth, during a period of rapid DRG development. This ring shape, which was only observed around larger neurons, is evidence that several satellite cells are synchronously activated. We found that sortilin, a transporter of a wide variety of intracellular proteins containing PSAP, is strongly localized to the inner side of satellite cells, which contact the neuronal surface. These findings suggest that PSAP and GPR37/GPR37L1 play a role in activating both satellite and nerve cells. |
| format |
article |
| author |
Miho Taniguchi Hiroaki Nabeka Kimiko Yamamiya Md Sakirul Islam Khan Tetsuya Shimokawa Farzana Islam Takuya Doihara Hiroyuki Wakisaka Naoto Kobayashi Fumihiko Hamada Seiji Matsuda |
| author_facet |
Miho Taniguchi Hiroaki Nabeka Kimiko Yamamiya Md Sakirul Islam Khan Tetsuya Shimokawa Farzana Islam Takuya Doihara Hiroyuki Wakisaka Naoto Kobayashi Fumihiko Hamada Seiji Matsuda |
| author_sort |
Miho Taniguchi |
| title |
The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion. |
| title_short |
The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion. |
| title_full |
The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion. |
| title_fullStr |
The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion. |
| title_full_unstemmed |
The expression of prosaposin and its receptors, GRP37 and GPR37L1, are increased in the developing dorsal root ganglion. |
| title_sort |
expression of prosaposin and its receptors, grp37 and gpr37l1, are increased in the developing dorsal root ganglion. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2021 |
| url |
https://doaj.org/article/f8c6f4a7e8654219b2bdbded91c774e1 |
| work_keys_str_mv |
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1718374323475972096 |