Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture

The aim of the work was to study the features of clonal micropropagation of essential oil rose interspecific hybrids obtained in embryo culture in vitro. Analysis of 12 crossing combinations demonstrated that the frequency of hybrid seedling formation in the embryo culture varied from 0 to 71.4%. Fo...

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Autores principales:	Yegorova Natalia, Stavtzeva Irina, Zolotilov Victor
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/f8c95c5cde6d44d0acf6cb1e3f068b46
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spelling	oai:doaj.org-article:f8c95c5cde6d44d0acf6cb1e3f068b462021-11-08T15:17:37ZMicropropagation in vitro of essential oil rose hybrids obtained in embryoculture2117-445810.1051/bioconf/20213800139https://doaj.org/article/f8c95c5cde6d44d0acf6cb1e3f068b462021-01-01T00:00:00Zhttps://www.bio-conferences.org/articles/bioconf/pdf/2021/10/bioconf_napd2021_00139.pdfhttps://doaj.org/toc/2117-4458The aim of the work was to study the features of clonal micropropagation of essential oil rose interspecific hybrids obtained in embryo culture in vitro. Analysis of 12 crossing combinations demonstrated that the frequency of hybrid seedling formation in the embryo culture varied from 0 to 71.4%. For clonal micropropagation obtained in vitro seedlings were divided into stem segments with a node and cultivated on MS culture medium supplemented with 0.5 mg/l BAP and 0.1 mg/l GA3. During the multiplication of 13 hybrids (R. alba × R. damascena cv. ‘Kazanlykskaya’) in 2-6 subcultures, high variability of the multiplication index (1.8-18.5 depending on the genotype and passage) was revealed. This parameter was maximum in the 3-4th subcultures. The best ability to micropropagation showed hybrid No. 37-14. Microshoots were rooted in vitro on ½ MS medium, containing for different hybrids 0.5 or 1.0 mg/l NAA; frequency – up to 80.5-100.0%. However, in No. 37-2, 37-19 and 37-31 on four tested media, the number of shoots with roots was only 0-35.4%.Yegorova NataliaStavtzeva IrinaZolotilov VictorEDP SciencesarticleMicrobiologyQR1-502PhysiologyQP1-981ZoologyQL1-991ENFRBIO Web of Conferences, Vol 38, p 00139 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
spellingShingle	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 Yegorova Natalia Stavtzeva Irina Zolotilov Victor Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture
description	The aim of the work was to study the features of clonal micropropagation of essential oil rose interspecific hybrids obtained in embryo culture in vitro. Analysis of 12 crossing combinations demonstrated that the frequency of hybrid seedling formation in the embryo culture varied from 0 to 71.4%. For clonal micropropagation obtained in vitro seedlings were divided into stem segments with a node and cultivated on MS culture medium supplemented with 0.5 mg/l BAP and 0.1 mg/l GA3. During the multiplication of 13 hybrids (R. alba × R. damascena cv. ‘Kazanlykskaya’) in 2-6 subcultures, high variability of the multiplication index (1.8-18.5 depending on the genotype and passage) was revealed. This parameter was maximum in the 3-4th subcultures. The best ability to micropropagation showed hybrid No. 37-14. Microshoots were rooted in vitro on ½ MS medium, containing for different hybrids 0.5 or 1.0 mg/l NAA; frequency – up to 80.5-100.0%. However, in No. 37-2, 37-19 and 37-31 on four tested media, the number of shoots with roots was only 0-35.4%.
format	article
author	Yegorova Natalia Stavtzeva Irina Zolotilov Victor
author_facet	Yegorova Natalia Stavtzeva Irina Zolotilov Victor
author_sort	Yegorova Natalia
title	Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture
title_short	Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture
title_full	Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture
title_fullStr	Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture
title_full_unstemmed	Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture
title_sort	micropropagation in vitro of essential oil rose hybrids obtained in embryoculture
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/f8c95c5cde6d44d0acf6cb1e3f068b46
work_keys_str_mv	AT yegorovanatalia micropropagationinvitroofessentialoilrosehybridsobtainedinembryoculture AT stavtzevairina micropropagationinvitroofessentialoilrosehybridsobtainedinembryoculture AT zolotilovvictor micropropagationinvitroofessentialoilrosehybridsobtainedinembryoculture
_version_	1718442103095164928

Micropropagation in vitro of essential oil rose hybrids obtained in embryoculture

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