Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

Kantha Deivi Arunachalam,1 Lilly Baptista Arun,1 Sathesh Kumar Annamalai,1 Aarrthy M Arunachalam2 1Center for Environmental Nuclear Research, SRM University, Potheri, Tamil Nadu, India; 2Division of Educational Services, Kaplan University (Medical), Washington DC, USA Background: Gymnema sylvestr...

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Autores principales:	Arunachalam KD, Arun LB, Annamalai SK, Arunachalam AM
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2014
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f8cb186cbbb1414baf0eb6e8af2a12ed
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spelling	oai:doaj.org-article:f8cb186cbbb1414baf0eb6e8af2a12ed2021-12-02T11:07:31ZPotential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles1178-2013https://doaj.org/article/f8cb186cbbb1414baf0eb6e8af2a12ed2014-12-01T00:00:00Zhttp://www.dovepress.com/potential-anticancer-properties-of-bioactive-compounds-of-gymnema-sylv-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Kantha Deivi Arunachalam,1 Lilly Baptista Arun,1 Sathesh Kumar Annamalai,1 Aarrthy M Arunachalam2 1Center for Environmental Nuclear Research, SRM University, Potheri, Tamil Nadu, India; 2Division of Educational Services, Kaplan University (Medical), Washington DC, USA Background: Gymnema sylvestre is an ethno-pharmacologically important medicinal plant used in many polyherbal formulations for its potential health benefits. Silver nanoparticles (SNPs) were biofunctionalized using aqueous leaf extracts of G. sylvestre. The anticancer properties of the bioactive compounds and the biofunctionalized SNPs were compared using the HT29 human adenoma colon cancer cell line.Methods: The preliminary phytochemical screening for bioactive compounds from aqueous extracts revealed the presence of alkaloids, triterpenes, flavonoids, steroids, and saponins. Biofunctionalized SNPs were synthesized using silver nitrate and characterized by ultraviolet–visible spectroscopy, scanning electron microscopy, energy-dispersive X-ray analysis, Fourier transform infrared spectroscopy, and X-ray diffraction for size and shape. The characterized biofunctionalized G. sylvestre were tested for its in vitro anticancer activity against HT29 human colon adenocarcinoma cells.Results: The biofunctionlized G. sylvestre SNPs showed the surface plasmon resonance band at 430 nm. The scanning electron microscopy images showed the presence of spherical nanoparticles of various sizes, which were further determined using the Scherrer equation. In vitro cytotoxic activity of the biofunctionalized green-synthesized SNPs (GSNPs) indicated that the sensitivity of HT29 human colon adenocarcinoma cells for cytotoxic drugs is higher than that of Vero cell line for the same cytotoxic agents and also higher than the bioactive compound of the aqueous extract.Conclusion: Our results show that the anticancer properties of the bioactive compounds of G. sylvestre can be enhanced through biofunctionalizing the SNPs using the bioactive compounds present in the plant extract without compromising their medicinal properties. Keywords: Gymnema sylvestre, gymnemic acid, biofunctionalized silver nanoparticles, anticancer activity, HT29 cell lineArunachalam KDArun LBAnnamalai SKArunachalam AMDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 31-41 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Arunachalam KD Arun LB Annamalai SK Arunachalam AM Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles
description	Kantha Deivi Arunachalam,1 Lilly Baptista Arun,1 Sathesh Kumar Annamalai,1 Aarrthy M Arunachalam2 1Center for Environmental Nuclear Research, SRM University, Potheri, Tamil Nadu, India; 2Division of Educational Services, Kaplan University (Medical), Washington DC, USA Background: Gymnema sylvestre is an ethno-pharmacologically important medicinal plant used in many polyherbal formulations for its potential health benefits. Silver nanoparticles (SNPs) were biofunctionalized using aqueous leaf extracts of G. sylvestre. The anticancer properties of the bioactive compounds and the biofunctionalized SNPs were compared using the HT29 human adenoma colon cancer cell line.Methods: The preliminary phytochemical screening for bioactive compounds from aqueous extracts revealed the presence of alkaloids, triterpenes, flavonoids, steroids, and saponins. Biofunctionalized SNPs were synthesized using silver nitrate and characterized by ultraviolet–visible spectroscopy, scanning electron microscopy, energy-dispersive X-ray analysis, Fourier transform infrared spectroscopy, and X-ray diffraction for size and shape. The characterized biofunctionalized G. sylvestre were tested for its in vitro anticancer activity against HT29 human colon adenocarcinoma cells.Results: The biofunctionlized G. sylvestre SNPs showed the surface plasmon resonance band at 430 nm. The scanning electron microscopy images showed the presence of spherical nanoparticles of various sizes, which were further determined using the Scherrer equation. In vitro cytotoxic activity of the biofunctionalized green-synthesized SNPs (GSNPs) indicated that the sensitivity of HT29 human colon adenocarcinoma cells for cytotoxic drugs is higher than that of Vero cell line for the same cytotoxic agents and also higher than the bioactive compound of the aqueous extract.Conclusion: Our results show that the anticancer properties of the bioactive compounds of G. sylvestre can be enhanced through biofunctionalizing the SNPs using the bioactive compounds present in the plant extract without compromising their medicinal properties. Keywords: Gymnema sylvestre, gymnemic acid, biofunctionalized silver nanoparticles, anticancer activity, HT29 cell line
format	article
author	Arunachalam KD Arun LB Annamalai SK Arunachalam AM
author_facet	Arunachalam KD Arun LB Annamalai SK Arunachalam AM
author_sort	Arunachalam KD
title	Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles
title_short	Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles
title_full	Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles
title_fullStr	Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles
title_full_unstemmed	Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles
title_sort	potential anticancer properties of bioactive compounds of gymnema sylvestre and its biofunctionalized silver nanoparticles
publisher	Dove Medical Press
publishDate	2014
url	https://doaj.org/article/f8cb186cbbb1414baf0eb6e8af2a12ed
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Potential anticancer properties of bioactive compounds of Gymnema sylvestre and its biofunctionalized silver nanoparticles

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