A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B

Abstract The human ABO blood group system is of great importance in blood transfusion and organ transplantation. ABO transcription is known to be regulated by a constitutive promoter in a CpG island and regions for regulation of cell-specific expression such as the downstream + 22.6-kb site for epit...

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Autores principales:	Rie Sano, Yoichiro Takahashi, Haruki Fukuda, Megumi Harada, Akira Hayakawa, Takafumi Okawa, Rieko Kubo, Haruo Takeshita, Junichi Tsukada, Yoshihiko Kominato
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8cba333df5646189eec22898a276701
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spelling	oai:doaj.org-article:f8cba333df5646189eec22898a2767012021-12-02T13:27:08ZA cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B10.1038/s41598-021-86843-62045-2322https://doaj.org/article/f8cba333df5646189eec22898a2767012021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86843-6https://doaj.org/toc/2045-2322Abstract The human ABO blood group system is of great importance in blood transfusion and organ transplantation. ABO transcription is known to be regulated by a constitutive promoter in a CpG island and regions for regulation of cell-specific expression such as the downstream + 22.6-kb site for epithelial cells and a site in intron 1 for erythroid cells. Here we investigated whether the + 22.6-kb site might play a role in transcriptional regulation of the gene encoding odorant binding protein 2B (OBP2B), which is located on the centromere side 43.4 kb from the + 22.6-kb site. In the gastric cancer cell line KATOIII, quantitative PCR analysis demonstrated significantly reduced amounts of OBP2B and ABO transcripts in mutant cells with biallelic deletions of the site created using the CRISPR/Cas9 system, relative to those in the wild-type cells, and Western blotting demonstrated a corresponding reduction of OBP2B protein in the mutant cells. Moreover, single-molecule fluorescence in situ hybridization assays indicated that the amounts of both transcripts were correlated in individual cells. These findings suggest that OBP2B could be co-regulated by the + 22.6-kb site of ABO.Rie SanoYoichiro TakahashiHaruki FukudaMegumi HaradaAkira HayakawaTakafumi OkawaRieko KuboHaruo TakeshitaJunichi TsukadaYoshihiko KominatoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rie Sano Yoichiro Takahashi Haruki Fukuda Megumi Harada Akira Hayakawa Takafumi Okawa Rieko Kubo Haruo Takeshita Junichi Tsukada Yoshihiko Kominato A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B
description	Abstract The human ABO blood group system is of great importance in blood transfusion and organ transplantation. ABO transcription is known to be regulated by a constitutive promoter in a CpG island and regions for regulation of cell-specific expression such as the downstream + 22.6-kb site for epithelial cells and a site in intron 1 for erythroid cells. Here we investigated whether the + 22.6-kb site might play a role in transcriptional regulation of the gene encoding odorant binding protein 2B (OBP2B), which is located on the centromere side 43.4 kb from the + 22.6-kb site. In the gastric cancer cell line KATOIII, quantitative PCR analysis demonstrated significantly reduced amounts of OBP2B and ABO transcripts in mutant cells with biallelic deletions of the site created using the CRISPR/Cas9 system, relative to those in the wild-type cells, and Western blotting demonstrated a corresponding reduction of OBP2B protein in the mutant cells. Moreover, single-molecule fluorescence in situ hybridization assays indicated that the amounts of both transcripts were correlated in individual cells. These findings suggest that OBP2B could be co-regulated by the + 22.6-kb site of ABO.
format	article
author	Rie Sano Yoichiro Takahashi Haruki Fukuda Megumi Harada Akira Hayakawa Takafumi Okawa Rieko Kubo Haruo Takeshita Junichi Tsukada Yoshihiko Kominato
author_facet	Rie Sano Yoichiro Takahashi Haruki Fukuda Megumi Harada Akira Hayakawa Takafumi Okawa Rieko Kubo Haruo Takeshita Junichi Tsukada Yoshihiko Kominato
author_sort	Rie Sano
title	A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B
title_short	A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B
title_full	A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B
title_fullStr	A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B
title_full_unstemmed	A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B
title_sort	cell-specific regulatory region of the human abo blood group gene regulates the neighborhood gene encoding odorant binding protein 2b
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f8cba333df5646189eec22898a276701
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A cell-specific regulatory region of the human ABO blood group gene regulates the neighborhood gene encoding odorant binding protein 2B

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