<i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians

SUMOylation and ubiquitylation are homologous processes catalyzed by homologous enzymes, and they are involved in nearly all aspects of eukaryotic biology. Planarians, which have the remarkable ability to regenerate their central nervous system (CNS), provide an excellent opportunity to investigate...

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Autores principales:	Qingnan Tian, Yujia Sun, Tingting Gao, Jiaxin Li, Huimin Fang, Shoutao Zhang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Djnedd4L</i> <i>Djubc9</i> stem cells regeneration SUMOylation ubiquitylation Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f8cffe421ff34b3e9c52d8bc94593aa6
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spelling	oai:doaj.org-article:f8cffe421ff34b3e9c52d8bc94593aa62021-11-11T17:10:05Z<i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians10.3390/ijms2221117071422-00671661-6596https://doaj.org/article/f8cffe421ff34b3e9c52d8bc94593aa62021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11707https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067SUMOylation and ubiquitylation are homologous processes catalyzed by homologous enzymes, and they are involved in nearly all aspects of eukaryotic biology. Planarians, which have the remarkable ability to regenerate their central nervous system (CNS), provide an excellent opportunity to investigate the molecular processes of CNS regeneration in vivo. In this study, we analyzed gene expression profiles during head regeneration with an RNA-seq-based screening approach and found that <i>Djnedd4L</i> and <i>Djubc9</i> were required for head regeneration in planarians. RNA interference targeting of <i>Djubc9</i> caused the phospho-H3 mitotic cells to decrease in quantity, or even become absent as a part of the <i>Djubc9</i> RNAi phenotype, which also showed the collapse of the stem cell lineage along with the reduced expression of epidermal differentiation markers. Furthermore, we found that <i>Djnedd4L</i> RNAi induced increased cell division and promoted the premature differentiation during regeneration. Taken together, our findings show that <i>Djubc9</i> and <i>Djnedd4L</i> are required for stem cell maintenance in the planarian <i>Dugesia japonica</i>, which helps to elucidate the role of SUMOylation and ubiquitylation in regulating the regeneration process.Qingnan TianYujia SunTingting GaoJiaxin LiHuimin FangShoutao ZhangMDPI AGarticle<i>Djnedd4L</i><i>Djubc9</i>stem cellsregenerationSUMOylationubiquitylationBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11707, p 11707 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Djnedd4L</i> <i>Djubc9</i> stem cells regeneration SUMOylation ubiquitylation Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	<i>Djnedd4L</i> <i>Djubc9</i> stem cells regeneration SUMOylation ubiquitylation Biology (General) QH301-705.5 Chemistry QD1-999 Qingnan Tian Yujia Sun Tingting Gao Jiaxin Li Huimin Fang Shoutao Zhang <i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians
description	SUMOylation and ubiquitylation are homologous processes catalyzed by homologous enzymes, and they are involved in nearly all aspects of eukaryotic biology. Planarians, which have the remarkable ability to regenerate their central nervous system (CNS), provide an excellent opportunity to investigate the molecular processes of CNS regeneration in vivo. In this study, we analyzed gene expression profiles during head regeneration with an RNA-seq-based screening approach and found that <i>Djnedd4L</i> and <i>Djubc9</i> were required for head regeneration in planarians. RNA interference targeting of <i>Djubc9</i> caused the phospho-H3 mitotic cells to decrease in quantity, or even become absent as a part of the <i>Djubc9</i> RNAi phenotype, which also showed the collapse of the stem cell lineage along with the reduced expression of epidermal differentiation markers. Furthermore, we found that <i>Djnedd4L</i> RNAi induced increased cell division and promoted the premature differentiation during regeneration. Taken together, our findings show that <i>Djubc9</i> and <i>Djnedd4L</i> are required for stem cell maintenance in the planarian <i>Dugesia japonica</i>, which helps to elucidate the role of SUMOylation and ubiquitylation in regulating the regeneration process.
format	article
author	Qingnan Tian Yujia Sun Tingting Gao Jiaxin Li Huimin Fang Shoutao Zhang
author_facet	Qingnan Tian Yujia Sun Tingting Gao Jiaxin Li Huimin Fang Shoutao Zhang
author_sort	Qingnan Tian
title	<i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians
title_short	<i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians
title_full	<i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians
title_fullStr	<i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians
title_full_unstemmed	<i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians
title_sort	<i>djnedd4l</i> is required for head regeneration by regulating stem cell maintenance in planarians
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f8cffe421ff34b3e9c52d8bc94593aa6
work_keys_str_mv	AT qingnantian idjnedd4liisrequiredforheadregenerationbyregulatingstemcellmaintenanceinplanarians AT yujiasun idjnedd4liisrequiredforheadregenerationbyregulatingstemcellmaintenanceinplanarians AT tingtinggao idjnedd4liisrequiredforheadregenerationbyregulatingstemcellmaintenanceinplanarians AT jiaxinli idjnedd4liisrequiredforheadregenerationbyregulatingstemcellmaintenanceinplanarians AT huiminfang idjnedd4liisrequiredforheadregenerationbyregulatingstemcellmaintenanceinplanarians AT shoutaozhang idjnedd4liisrequiredforheadregenerationbyregulatingstemcellmaintenanceinplanarians
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<i>Djnedd4L</i> Is Required for Head Regeneration by Regulating Stem Cell Maintenance in Planarians

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