Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.

To extend our understanding of flowering time control in eudicots, we screened for mutants in the model legume Medicago truncatula (Medicago). We identified an early flowering mutant, spring1, in a T-DNA mutant screen, but spring1 was not tagged and was deemed a somaclonal mutant. We backcrossed the...

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Autores principales:	Chin Chin Yeoh, Martin Balcerowicz, Lulu Zhang, Mauren Jaudal, Lysiane Brocard, Pascal Ratet, Joanna Putterill
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8d59021a88246fd85ee887985949b72
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spelling	oai:doaj.org-article:f8d59021a88246fd85ee887985949b722021-11-18T08:02:29ZFine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.1932-620310.1371/journal.pone.0053467https://doaj.org/article/f8d59021a88246fd85ee887985949b722013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23308229/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203To extend our understanding of flowering time control in eudicots, we screened for mutants in the model legume Medicago truncatula (Medicago). We identified an early flowering mutant, spring1, in a T-DNA mutant screen, but spring1 was not tagged and was deemed a somaclonal mutant. We backcrossed the mutant to wild type R108. The F1 plants and the majority of F2 plants were early flowering like spring1, strongly indicating that spring1 conferred monogenic, dominant early flowering. We hypothesized that the spring1 phenotype resulted from over expression of an activator of flowering. Previously, a major QTL for flowering time in different Medicago accessions was located to an interval on chromosome 7 with six candidate flowering-time activators, including a CONSTANS gene, MtCO, and three FLOWERING LOCUS T (FT) genes. Hence we embarked upon linkage mapping using 29 markers from the MtCO/FT region on chromosome 7 on two populations developed by crossing spring1 with Jester. Spring1 mapped to an interval of ∼0.5 Mb on chromosome 7 that excluded MtCO, but contained 78 genes, including the three FT genes. Of these FT genes, only FTa1 was up-regulated in spring1 plants. We then investigated global gene expression in spring1 and R108 by microarray analysis. Overall, they had highly similar gene expression and apart from FTa1, no genes in the mapping interval were differentially expressed. Two MADS transcription factor genes, FRUITFULLb (FULb) and SUPPRESSOR OF OVER EXPRESSION OF CONSTANS1a (SOC1a), that were up-regulated in spring1, were also up-regulated in transgenic Medicago over-expressing FTa1. This suggested that their differential expression in spring1 resulted from the increased abundance of FTa1. A 6255 bp genomic FTa1 fragment, including the complete 5' region, was sequenced, but no changes were observed indicating that the spring1 mutation is not a DNA sequence difference in the FTa1 promoter or introns.Chin Chin YeohMartin BalcerowiczLulu ZhangMauren JaudalLysiane BrocardPascal RatetJoanna PutterillPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53467 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chin Chin Yeoh Martin Balcerowicz Lulu Zhang Mauren Jaudal Lysiane Brocard Pascal Ratet Joanna Putterill Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.
description	To extend our understanding of flowering time control in eudicots, we screened for mutants in the model legume Medicago truncatula (Medicago). We identified an early flowering mutant, spring1, in a T-DNA mutant screen, but spring1 was not tagged and was deemed a somaclonal mutant. We backcrossed the mutant to wild type R108. The F1 plants and the majority of F2 plants were early flowering like spring1, strongly indicating that spring1 conferred monogenic, dominant early flowering. We hypothesized that the spring1 phenotype resulted from over expression of an activator of flowering. Previously, a major QTL for flowering time in different Medicago accessions was located to an interval on chromosome 7 with six candidate flowering-time activators, including a CONSTANS gene, MtCO, and three FLOWERING LOCUS T (FT) genes. Hence we embarked upon linkage mapping using 29 markers from the MtCO/FT region on chromosome 7 on two populations developed by crossing spring1 with Jester. Spring1 mapped to an interval of ∼0.5 Mb on chromosome 7 that excluded MtCO, but contained 78 genes, including the three FT genes. Of these FT genes, only FTa1 was up-regulated in spring1 plants. We then investigated global gene expression in spring1 and R108 by microarray analysis. Overall, they had highly similar gene expression and apart from FTa1, no genes in the mapping interval were differentially expressed. Two MADS transcription factor genes, FRUITFULLb (FULb) and SUPPRESSOR OF OVER EXPRESSION OF CONSTANS1a (SOC1a), that were up-regulated in spring1, were also up-regulated in transgenic Medicago over-expressing FTa1. This suggested that their differential expression in spring1 resulted from the increased abundance of FTa1. A 6255 bp genomic FTa1 fragment, including the complete 5' region, was sequenced, but no changes were observed indicating that the spring1 mutation is not a DNA sequence difference in the FTa1 promoter or introns.
format	article
author	Chin Chin Yeoh Martin Balcerowicz Lulu Zhang Mauren Jaudal Lysiane Brocard Pascal Ratet Joanna Putterill
author_facet	Chin Chin Yeoh Martin Balcerowicz Lulu Zhang Mauren Jaudal Lysiane Brocard Pascal Ratet Joanna Putterill
author_sort	Chin Chin Yeoh
title	Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.
title_short	Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.
title_full	Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.
title_fullStr	Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.
title_full_unstemmed	Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.
title_sort	fine mapping links the fta1 flowering time regulator to the dominant spring1 locus in medicago.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/f8d59021a88246fd85ee887985949b72
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Fine mapping links the FTa1 flowering time regulator to the dominant spring1 locus in Medicago.

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