Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>
Rice false smut caused by <i>Ustilaginoidea virens</i> is a serious disease of rice (<i>Oryza sativa</i>), severely reducing plant mass and yields worldwide. We performed genome-wide analysis of the CCHC-type zinc-finger transcription factor family in this pathogen. We identi...
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| Autores principales: | , , , , , , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/f8e0d292b4994500ba4f1c2e134a9ee8 |
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| Sumario: | Rice false smut caused by <i>Ustilaginoidea virens</i> is a serious disease of rice (<i>Oryza sativa</i>), severely reducing plant mass and yields worldwide. We performed genome-wide analysis of the CCHC-type zinc-finger transcription factor family in this pathogen. We identified and functionally characterized seven <i>UvCCHC</i> genes in <i>U. virens</i>. The deletion of various <i>UvCCHC</i> genes affected the stress responses, vegetative growth, conidiation, and virulence of <i>U. virens</i>. ∆<i>UvCCHC5</i> mutants infected rice spikelets normally but could not form smut balls. Sugar utilization experiments showed that the ∆<i>UvCCHC5</i> mutants were defective in the utilization of glucose, sucrose, lactose, stachyose, and trehalose. Deletion of <i>UvCCHC5</i> did not affect the expression of rice genes associated with grain filling, as revealed by RT-qPCR. We propose that the ∆<i>UvCCHC5</i> mutants are impaired in transmembrane transport, and the resulting nutrient deficiencies prevent them from using nutrients from rice to form smut balls. RNA-seq data analysis indicated that <i>UvCCHC4</i> affects the expression of genes involved in mitochondrial biogenesis, ribosomes, transporters, and ribosome biogenesis. These findings improve our understanding of the molecular mechanism underlying smut ball formation in rice by <i>U. virens</i>. |
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