Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in Ustilaginoidea virens

Rice false smut caused by Ustilaginoidea virens is a serious disease of rice (Oryza sativa), severely reducing plant mass and yields worldwide. We performed genome-wide analysis of the CCHC-type zinc-finger transcription factor family in this pathogen. We identi...

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Autores principales:	Xiaoyang Chen, Zhangxin Pei, Lin Peng, Qin Qin, Yuhang Duan, Hao Liu, Xiaolin Chen, Lu Zheng, Chaoxi Luo, Junbin Huang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	rice false smut <i>Ustilaginoidea virens</i> CCHC-type zinc finger proteins development virulence Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/f8e0d292b4994500ba4f1c2e134a9ee8
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spelling	oai:doaj.org-article:f8e0d292b4994500ba4f1c2e134a9ee82021-11-25T18:06:03ZGenome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>10.3390/jof71109472309-608Xhttps://doaj.org/article/f8e0d292b4994500ba4f1c2e134a9ee82021-11-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/947https://doaj.org/toc/2309-608XRice false smut caused by <i>Ustilaginoidea virens</i> is a serious disease of rice (<i>Oryza sativa</i>), severely reducing plant mass and yields worldwide. We performed genome-wide analysis of the CCHC-type zinc-finger transcription factor family in this pathogen. We identified and functionally characterized seven <i>UvCCHC</i> genes in <i>U. virens</i>. The deletion of various <i>UvCCHC</i> genes affected the stress responses, vegetative growth, conidiation, and virulence of <i>U. virens</i>. ∆<i>UvCCHC5</i> mutants infected rice spikelets normally but could not form smut balls. Sugar utilization experiments showed that the ∆<i>UvCCHC5</i> mutants were defective in the utilization of glucose, sucrose, lactose, stachyose, and trehalose. Deletion of <i>UvCCHC5</i> did not affect the expression of rice genes associated with grain filling, as revealed by RT-qPCR. We propose that the ∆<i>UvCCHC5</i> mutants are impaired in transmembrane transport, and the resulting nutrient deficiencies prevent them from using nutrients from rice to form smut balls. RNA-seq data analysis indicated that <i>UvCCHC4</i> affects the expression of genes involved in mitochondrial biogenesis, ribosomes, transporters, and ribosome biogenesis. These findings improve our understanding of the molecular mechanism underlying smut ball formation in rice by <i>U. virens</i>.Xiaoyang ChenZhangxin PeiLin PengQin QinYuhang DuanHao LiuXiaolin ChenLu ZhengChaoxi LuoJunbin HuangMDPI AGarticlerice false smut<i>Ustilaginoidea virens</i>CCHC-type zinc finger proteinsdevelopmentvirulenceBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 947, p 947 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rice false smut <i>Ustilaginoidea virens</i> CCHC-type zinc finger proteins development virulence Biology (General) QH301-705.5
spellingShingle	rice false smut <i>Ustilaginoidea virens</i> CCHC-type zinc finger proteins development virulence Biology (General) QH301-705.5 Xiaoyang Chen Zhangxin Pei Lin Peng Qin Qin Yuhang Duan Hao Liu Xiaolin Chen Lu Zheng Chaoxi Luo Junbin Huang Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>
description	Rice false smut caused by <i>Ustilaginoidea virens</i> is a serious disease of rice (<i>Oryza sativa</i>), severely reducing plant mass and yields worldwide. We performed genome-wide analysis of the CCHC-type zinc-finger transcription factor family in this pathogen. We identified and functionally characterized seven <i>UvCCHC</i> genes in <i>U. virens</i>. The deletion of various <i>UvCCHC</i> genes affected the stress responses, vegetative growth, conidiation, and virulence of <i>U. virens</i>. ∆<i>UvCCHC5</i> mutants infected rice spikelets normally but could not form smut balls. Sugar utilization experiments showed that the ∆<i>UvCCHC5</i> mutants were defective in the utilization of glucose, sucrose, lactose, stachyose, and trehalose. Deletion of <i>UvCCHC5</i> did not affect the expression of rice genes associated with grain filling, as revealed by RT-qPCR. We propose that the ∆<i>UvCCHC5</i> mutants are impaired in transmembrane transport, and the resulting nutrient deficiencies prevent them from using nutrients from rice to form smut balls. RNA-seq data analysis indicated that <i>UvCCHC4</i> affects the expression of genes involved in mitochondrial biogenesis, ribosomes, transporters, and ribosome biogenesis. These findings improve our understanding of the molecular mechanism underlying smut ball formation in rice by <i>U. virens</i>.
format	article
author	Xiaoyang Chen Zhangxin Pei Lin Peng Qin Qin Yuhang Duan Hao Liu Xiaolin Chen Lu Zheng Chaoxi Luo Junbin Huang
author_facet	Xiaoyang Chen Zhangxin Pei Lin Peng Qin Qin Yuhang Duan Hao Liu Xiaolin Chen Lu Zheng Chaoxi Luo Junbin Huang
author_sort	Xiaoyang Chen
title	Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>
title_short	Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>
title_full	Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>
title_fullStr	Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>
title_full_unstemmed	Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>
title_sort	genome-wide identification and functional characterization of cchc-type zinc finger genes in <i>ustilaginoidea virens</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f8e0d292b4994500ba4f1c2e134a9ee8
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Genome-Wide Identification and Functional Characterization of CCHC-Type Zinc Finger Genes in <i>Ustilaginoidea virens</i>

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