DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
Abstract The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,40...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/f8e27d7d62fb499faa7a487ea347b299 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:f8e27d7d62fb499faa7a487ea347b299 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:f8e27d7d62fb499faa7a487ea347b2992021-12-02T17:08:43ZDNA damage in circulating leukocytes measured with the comet assay may predict the risk of death10.1038/s41598-021-95976-72045-2322https://doaj.org/article/f8e27d7d62fb499faa7a487ea347b2992021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95976-7https://doaj.org/toc/2045-2322Abstract The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.Stefano BonassiMarcello CeppiPeter MøllerAmaya AzquetaMirta MilićNeri MonicaGunnar BrunborgRoger GodschalkGudrun KoppenSabine A. S. LangieJoão Paulo TeixeiraMarco BruzzoneJuliana Da SilvaDanieli BenedettiDelia CavalloCinzia Lucia UrsiniLisa GiovannelliSilvia MorettiPatrizia RisoCristian Del Bo’Patrizia RussoMalgorzata DobrzyńskaIrina A. GoroshinskayaEkaterina I. SurikovaMarta StaruchovaMagdalena BarančokovaKatarina VolkovovaAlena KažimirovaBozena SmolkovaBlanca LaffonVanessa ValdiglesiasSusana PastorRicard MarcosAlba HernándezGoran GajskiBiljana Spremo-PotparevićLada ŽivkovićElisa Boutet-RobinetHervé PerdryPierre LebaillyCarlos L. PerezNursen BasaranZsuzsanna NemethAnna SafarMaria DusinskaAndrew Collinsfor the hCOMET projectNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Stefano Bonassi Marcello Ceppi Peter Møller Amaya Azqueta Mirta Milić Neri Monica Gunnar Brunborg Roger Godschalk Gudrun Koppen Sabine A. S. Langie João Paulo Teixeira Marco Bruzzone Juliana Da Silva Danieli Benedetti Delia Cavallo Cinzia Lucia Ursini Lisa Giovannelli Silvia Moretti Patrizia Riso Cristian Del Bo’ Patrizia Russo Malgorzata Dobrzyńska Irina A. Goroshinskaya Ekaterina I. Surikova Marta Staruchova Magdalena Barančokova Katarina Volkovova Alena Kažimirova Bozena Smolkova Blanca Laffon Vanessa Valdiglesias Susana Pastor Ricard Marcos Alba Hernández Goran Gajski Biljana Spremo-Potparević Lada Živković Elisa Boutet-Robinet Hervé Perdry Pierre Lebailly Carlos L. Perez Nursen Basaran Zsuzsanna Nemeth Anna Safar Maria Dusinska Andrew Collins for the hCOMET project DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| description |
Abstract The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases. |
| format |
article |
| author |
Stefano Bonassi Marcello Ceppi Peter Møller Amaya Azqueta Mirta Milić Neri Monica Gunnar Brunborg Roger Godschalk Gudrun Koppen Sabine A. S. Langie João Paulo Teixeira Marco Bruzzone Juliana Da Silva Danieli Benedetti Delia Cavallo Cinzia Lucia Ursini Lisa Giovannelli Silvia Moretti Patrizia Riso Cristian Del Bo’ Patrizia Russo Malgorzata Dobrzyńska Irina A. Goroshinskaya Ekaterina I. Surikova Marta Staruchova Magdalena Barančokova Katarina Volkovova Alena Kažimirova Bozena Smolkova Blanca Laffon Vanessa Valdiglesias Susana Pastor Ricard Marcos Alba Hernández Goran Gajski Biljana Spremo-Potparević Lada Živković Elisa Boutet-Robinet Hervé Perdry Pierre Lebailly Carlos L. Perez Nursen Basaran Zsuzsanna Nemeth Anna Safar Maria Dusinska Andrew Collins for the hCOMET project |
| author_facet |
Stefano Bonassi Marcello Ceppi Peter Møller Amaya Azqueta Mirta Milić Neri Monica Gunnar Brunborg Roger Godschalk Gudrun Koppen Sabine A. S. Langie João Paulo Teixeira Marco Bruzzone Juliana Da Silva Danieli Benedetti Delia Cavallo Cinzia Lucia Ursini Lisa Giovannelli Silvia Moretti Patrizia Riso Cristian Del Bo’ Patrizia Russo Malgorzata Dobrzyńska Irina A. Goroshinskaya Ekaterina I. Surikova Marta Staruchova Magdalena Barančokova Katarina Volkovova Alena Kažimirova Bozena Smolkova Blanca Laffon Vanessa Valdiglesias Susana Pastor Ricard Marcos Alba Hernández Goran Gajski Biljana Spremo-Potparević Lada Živković Elisa Boutet-Robinet Hervé Perdry Pierre Lebailly Carlos L. Perez Nursen Basaran Zsuzsanna Nemeth Anna Safar Maria Dusinska Andrew Collins for the hCOMET project |
| author_sort |
Stefano Bonassi |
| title |
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_short |
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_full |
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_fullStr |
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_full_unstemmed |
DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| title_sort |
dna damage in circulating leukocytes measured with the comet assay may predict the risk of death |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/f8e27d7d62fb499faa7a487ea347b299 |
| work_keys_str_mv |
AT stefanobonassi dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT marcelloceppi dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT petermøller dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT amayaazqueta dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT mirtamilic dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT nerimonica dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT gunnarbrunborg dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT rogergodschalk dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT gudrunkoppen dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT sabineaslangie dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT joaopauloteixeira dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT marcobruzzone dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT julianadasilva dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT danielibenedetti dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT deliacavallo dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT cinzialuciaursini dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT lisagiovannelli dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT silviamoretti dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT patriziariso dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT cristiandelbo dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT patriziarusso dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT malgorzatadobrzynska dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT irinaagoroshinskaya dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT ekaterinaisurikova dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT martastaruchova dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT magdalenabarancokova dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT katarinavolkovova dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT alenakazimirova dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT bozenasmolkova dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT blancalaffon dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT vanessavaldiglesias dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT susanapastor dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT ricardmarcos dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT albahernandez dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT gorangajski dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT biljanaspremopotparevic dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT ladazivkovic dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT elisaboutetrobinet dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT herveperdry dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT pierrelebailly dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT carloslperez dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT nursenbasaran dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT zsuzsannanemeth dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT annasafar dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT mariadusinska dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT andrewcollins dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath AT forthehcometproject dnadamageincirculatingleukocytesmeasuredwiththecometassaymaypredicttheriskofdeath |
| _version_ |
1718381533920755712 |