Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola

Corynespora cassiicola, the causal agent of an extensive range of plant diseases worldwide, is a momentous fungus with diverse lifestyles and rich in intraspecies variations. In the present study, a total of 56 mitochondrial genomes of C. cassiicola were assembled (except two available online) and a...

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Autores principales:	Qingzhou Ma, Haiyan Wu, Yuehua Geng, Qiang Li, Rui Zang, Yashuang Guo, Chao Xu, Meng Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Corynespora cassiicola Mitochondrial genome Intraspecific comparison Group I intron mtDNA Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/f8e3502ea8684235ac6a63267bfcf489
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spelling	oai:doaj.org-article:f8e3502ea8684235ac6a63267bfcf4892021-11-14T04:31:41ZMitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola2001-037010.1016/j.csbj.2021.11.002https://doaj.org/article/f8e3502ea8684235ac6a63267bfcf4892021-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2001037021004670https://doaj.org/toc/2001-0370Corynespora cassiicola, the causal agent of an extensive range of plant diseases worldwide, is a momentous fungus with diverse lifestyles and rich in intraspecies variations. In the present study, a total of 56 mitochondrial genomes of C. cassiicola were assembled (except two available online) and analyzed, of which 16 mitogenomes were newly sequenced here. All these circular mitochondrial DNA (mtDNA) molecules, ranging from 39,223 bp to 45,786 bp in length, comprised the same set of 13 core protein-coding genes (PCGs), two rRNAs and 27 tRNAs arranged in identical order. Across the above conserved genes, nad3 had the largest genetic distance between different isolates and was possibly subjected to positive selection pressure. Comparative mitogenomic analysis indicated that seven group I (IB, IC1, and IC2) introns with a length range of 1013–1876 bp were differentially inserted in three core PCGs (cox1, nad1, and nad5), resulting in the varied mitogenome sizes among C. cassiicola isolates. In combination with dynamic distribution of the introns, a well-supported mitogenome-wide phylogeny of the 56 C. cassiicola isolates revealed eight phylogenetic groups, which only had weak correlations with host range and toxin class. Different groups of isolates exhibited obvious differences in length and GC content of some genes, while a degree of variance in codon usage and tRNA structure was also observed. This research served as the first report on mitogenomic comparisons within C. cassiicola, and could provide new insights into its intraspecific microevolution and genetic diversity.Qingzhou MaHaiyan WuYuehua GengQiang LiRui ZangYashuang GuoChao XuMeng ZhangElsevierarticleCorynespora cassiicolaMitochondrial genomeIntraspecific comparisonGroup I intronmtDNABiotechnologyTP248.13-248.65ENComputational and Structural Biotechnology Journal, Vol 19, Iss , Pp 5987-5999 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Corynespora cassiicola Mitochondrial genome Intraspecific comparison Group I intron mtDNA Biotechnology TP248.13-248.65
spellingShingle	Corynespora cassiicola Mitochondrial genome Intraspecific comparison Group I intron mtDNA Biotechnology TP248.13-248.65 Qingzhou Ma Haiyan Wu Yuehua Geng Qiang Li Rui Zang Yashuang Guo Chao Xu Meng Zhang Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola
description	Corynespora cassiicola, the causal agent of an extensive range of plant diseases worldwide, is a momentous fungus with diverse lifestyles and rich in intraspecies variations. In the present study, a total of 56 mitochondrial genomes of C. cassiicola were assembled (except two available online) and analyzed, of which 16 mitogenomes were newly sequenced here. All these circular mitochondrial DNA (mtDNA) molecules, ranging from 39,223 bp to 45,786 bp in length, comprised the same set of 13 core protein-coding genes (PCGs), two rRNAs and 27 tRNAs arranged in identical order. Across the above conserved genes, nad3 had the largest genetic distance between different isolates and was possibly subjected to positive selection pressure. Comparative mitogenomic analysis indicated that seven group I (IB, IC1, and IC2) introns with a length range of 1013–1876 bp were differentially inserted in three core PCGs (cox1, nad1, and nad5), resulting in the varied mitogenome sizes among C. cassiicola isolates. In combination with dynamic distribution of the introns, a well-supported mitogenome-wide phylogeny of the 56 C. cassiicola isolates revealed eight phylogenetic groups, which only had weak correlations with host range and toxin class. Different groups of isolates exhibited obvious differences in length and GC content of some genes, while a degree of variance in codon usage and tRNA structure was also observed. This research served as the first report on mitogenomic comparisons within C. cassiicola, and could provide new insights into its intraspecific microevolution and genetic diversity.
format	article
author	Qingzhou Ma Haiyan Wu Yuehua Geng Qiang Li Rui Zang Yashuang Guo Chao Xu Meng Zhang
author_facet	Qingzhou Ma Haiyan Wu Yuehua Geng Qiang Li Rui Zang Yashuang Guo Chao Xu Meng Zhang
author_sort	Qingzhou Ma
title	Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola
title_short	Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola
title_full	Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola
title_fullStr	Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola
title_full_unstemmed	Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola
title_sort	mitogenome-wide comparison and phylogeny reveal group i intron dynamics and intraspecific diversification within the phytopathogen corynespora cassiicola
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/f8e3502ea8684235ac6a63267bfcf489
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Mitogenome-wide comparison and phylogeny reveal group I intron dynamics and intraspecific diversification within the phytopathogen Corynespora cassiicola

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