Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer

Muscle invasive bladder cancer (MIBC) is a malignancy with considerable heterogeneity. The MIBC tumor microenvironment (TME) is highly complex, comprising diverse phenotypes and spatial architectures. The complexity of the MIBC TME must be characterized to provide potential targets for precision the...

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Autores principales:	Chao Feng, Xi Wang, Yuting Tao, Yuanliang Xie, Zhiyong Lai, Zhijian Li, Jiaxin Hu, Shaomei Tang, Lixin Pan, Liangyu He, Qiuyan Wang, Tianyu Li, Zengnan Mo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	muscle invasive bladder cancer tumor microenvironment cancer stem cell mass cytometry imaging mass cytometry Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/f8e58b83b81d4c3f8076577e4cfadcef
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spelling	oai:doaj.org-article:f8e58b83b81d4c3f8076577e4cfadcef2021-11-11T15:32:24ZSingle-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer10.3390/cancers132154402072-6694https://doaj.org/article/f8e58b83b81d4c3f8076577e4cfadcef2021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5440https://doaj.org/toc/2072-6694Muscle invasive bladder cancer (MIBC) is a malignancy with considerable heterogeneity. The MIBC tumor microenvironment (TME) is highly complex, comprising diverse phenotypes and spatial architectures. The complexity of the MIBC TME must be characterized to provide potential targets for precision therapy. Herein, an integrated combination of mass cytometry and imaging mass cytometry was used to analyze tumor cells, immune cells, and TME spatial characteristics of 44 MIBC patients. We detected tumor and immune cell clusters with abnormal phenotypes. In particular, we identified a previously overlooked cancer stem-like cell cluster (ALDH<sup>+</sup>PD-L1<sup>+</sup>ER-β<sup>−</sup>) that was strongly associated with poor prognosis. We elucidated the different spatial architectures of immune cells (excluded, infiltrated, and deserted) and tumor-associated collagens (curved, stretched, directionally distributed, and chaotic) in the MIBC TME. The present study is the first to provide in-depth insight into the complexity of the MIBC TME at the single-cell level. Our results will improve the general understanding of the heterogeneous characteristics of MIBC, potentially facilitating patient stratification and personalized therapy.Chao FengXi WangYuting TaoYuanliang XieZhiyong LaiZhijian LiJiaxin HuShaomei TangLixin PanLiangyu HeQiuyan WangTianyu LiZengnan MoMDPI AGarticlemuscle invasive bladder cancertumor microenvironmentcancer stem cellmass cytometryimaging mass cytometryNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5440, p 5440 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	muscle invasive bladder cancer tumor microenvironment cancer stem cell mass cytometry imaging mass cytometry Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	muscle invasive bladder cancer tumor microenvironment cancer stem cell mass cytometry imaging mass cytometry Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Chao Feng Xi Wang Yuting Tao Yuanliang Xie Zhiyong Lai Zhijian Li Jiaxin Hu Shaomei Tang Lixin Pan Liangyu He Qiuyan Wang Tianyu Li Zengnan Mo Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer
description	Muscle invasive bladder cancer (MIBC) is a malignancy with considerable heterogeneity. The MIBC tumor microenvironment (TME) is highly complex, comprising diverse phenotypes and spatial architectures. The complexity of the MIBC TME must be characterized to provide potential targets for precision therapy. Herein, an integrated combination of mass cytometry and imaging mass cytometry was used to analyze tumor cells, immune cells, and TME spatial characteristics of 44 MIBC patients. We detected tumor and immune cell clusters with abnormal phenotypes. In particular, we identified a previously overlooked cancer stem-like cell cluster (ALDH<sup>+</sup>PD-L1<sup>+</sup>ER-β<sup>−</sup>) that was strongly associated with poor prognosis. We elucidated the different spatial architectures of immune cells (excluded, infiltrated, and deserted) and tumor-associated collagens (curved, stretched, directionally distributed, and chaotic) in the MIBC TME. The present study is the first to provide in-depth insight into the complexity of the MIBC TME at the single-cell level. Our results will improve the general understanding of the heterogeneous characteristics of MIBC, potentially facilitating patient stratification and personalized therapy.
format	article
author	Chao Feng Xi Wang Yuting Tao Yuanliang Xie Zhiyong Lai Zhijian Li Jiaxin Hu Shaomei Tang Lixin Pan Liangyu He Qiuyan Wang Tianyu Li Zengnan Mo
author_facet	Chao Feng Xi Wang Yuting Tao Yuanliang Xie Zhiyong Lai Zhijian Li Jiaxin Hu Shaomei Tang Lixin Pan Liangyu He Qiuyan Wang Tianyu Li Zengnan Mo
author_sort	Chao Feng
title	Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer
title_short	Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer
title_full	Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer
title_fullStr	Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer
title_full_unstemmed	Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer
title_sort	single-cell proteomic analysis dissects the complexity of tumor microenvironment in muscle invasive bladder cancer
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f8e58b83b81d4c3f8076577e4cfadcef
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Single-Cell Proteomic Analysis Dissects the Complexity of Tumor Microenvironment in Muscle Invasive Bladder Cancer

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