Analyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans

Streptococcus mutans serotype k strains comprise <3% of oral isolates of S. mutans but are prominent in diseased cardiovascular (CV) tissue. Collagen binding protein (CBP) genes, cbm and cnm, are prevalent in serotype k strains and are associated with endothelial cell invasion. Nicotine incre...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dawn R. Wagenknecht, Richard L. Gregory
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	cbm gene cnm gene arginine nicotine Streptococcus mutans biofilm Dentistry RK1-715
Acceso en línea:	https://doaj.org/article/f8f361b050104ffaa144b21a45c31ad3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f8f361b050104ffaa144b21a45c31ad3
record_format	dspace
spelling	oai:doaj.org-article:f8f361b050104ffaa144b21a45c31ad32021-11-30T22:09:16ZAnalyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans2673-484210.3389/froh.2021.764784https://doaj.org/article/f8f361b050104ffaa144b21a45c31ad32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/froh.2021.764784/fullhttps://doaj.org/toc/2673-4842Streptococcus mutans serotype k strains comprise <3% of oral isolates of S. mutans but are prominent in diseased cardiovascular (CV) tissue. Collagen binding protein (CBP) genes, cbm and cnm, are prevalent in serotype k strains and are associated with endothelial cell invasion. Nicotine increases biofilm formation by serotype c strains of S. mutans, but its effects on serotype k strains and strains with CBP are unknown. Saliva contains arginine which alters certain properties of the extracellular polysaccharides (EPS) in S. mutans biofilm. We examined whether nicotine and arginine affect sucrose-induced biofilm of S. mutans serotypes k (n = 23) and c (n = 10) strains with and without CBP genes. Biofilm mass, metabolism, bacterial proliferation, and EPS production were assessed. Nicotine increased biomass and metabolic activity (p < 0.0001); arginine alone had no effect. The presence of a CBP gene (either cbm or cnm) had a significant effect on biofilm production, but serotype did not. Nicotine increased bacterial proliferation and the effect was greater in CBP + strains compared to strains lacking CBP genes. Addition of arginine with nicotine decreased both bacterial mass and EPS compared to biofilm grown in nicotine alone. EPS production was greater in cnm + than cbm + strains (p < 0.0001). Given the findings of S. mutans in diseased CV tissue, a nicotine induced increase in biofilm production by CBP + strains may be a key link between tobacco use and CV diseases.Dawn R. WagenknechtRichard L. GregoryFrontiers Media S.A.articlecbm genecnm geneargininenicotineStreptococcus mutansbiofilmDentistryRK1-715ENFrontiers in Oral Health, Vol 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	cbm gene cnm gene arginine nicotine Streptococcus mutans biofilm Dentistry RK1-715
spellingShingle	cbm gene cnm gene arginine nicotine Streptococcus mutans biofilm Dentistry RK1-715 Dawn R. Wagenknecht Richard L. Gregory Analyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans
description	Streptococcus mutans serotype k strains comprise <3% of oral isolates of S. mutans but are prominent in diseased cardiovascular (CV) tissue. Collagen binding protein (CBP) genes, cbm and cnm, are prevalent in serotype k strains and are associated with endothelial cell invasion. Nicotine increases biofilm formation by serotype c strains of S. mutans, but its effects on serotype k strains and strains with CBP are unknown. Saliva contains arginine which alters certain properties of the extracellular polysaccharides (EPS) in S. mutans biofilm. We examined whether nicotine and arginine affect sucrose-induced biofilm of S. mutans serotypes k (n = 23) and c (n = 10) strains with and without CBP genes. Biofilm mass, metabolism, bacterial proliferation, and EPS production were assessed. Nicotine increased biomass and metabolic activity (p < 0.0001); arginine alone had no effect. The presence of a CBP gene (either cbm or cnm) had a significant effect on biofilm production, but serotype did not. Nicotine increased bacterial proliferation and the effect was greater in CBP + strains compared to strains lacking CBP genes. Addition of arginine with nicotine decreased both bacterial mass and EPS compared to biofilm grown in nicotine alone. EPS production was greater in cnm + than cbm + strains (p < 0.0001). Given the findings of S. mutans in diseased CV tissue, a nicotine induced increase in biofilm production by CBP + strains may be a key link between tobacco use and CV diseases.
format	article
author	Dawn R. Wagenknecht Richard L. Gregory
author_facet	Dawn R. Wagenknecht Richard L. Gregory
author_sort	Dawn R. Wagenknecht
title	Analyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans
title_short	Analyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans
title_full	Analyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans
title_fullStr	Analyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans
title_full_unstemmed	Analyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans
title_sort	analyses of the effects of arginine, nicotine, serotype and collagen-binding proteins on biofilm development by 33 strains of streptococcus mutans
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/f8f361b050104ffaa144b21a45c31ad3
work_keys_str_mv	AT dawnrwagenknecht analysesoftheeffectsofargininenicotineserotypeandcollagenbindingproteinsonbiofilmdevelopmentby33strainsofstreptococcusmutans AT richardlgregory analysesoftheeffectsofargininenicotineserotypeandcollagenbindingproteinsonbiofilmdevelopmentby33strainsofstreptococcusmutans
_version_	1718406299640659968

Analyses of the Effects of Arginine, Nicotine, Serotype and Collagen-Binding Proteins on Biofilm Development by 33 Strains of Streptococcus mutans

Ejemplares similares