Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice

Abstract Fibroblast growth factor 2 (FGF2) is important in musculoskeletal homeostasis, therefore the impact of reduction or Fgf2 knockout on skeletal muscle function and phenotype was determined. Gait analysis as well as muscle strength testing in young and old WT and Fgf2KO demonstrated age-relate...

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Autores principales:	C. Homer-Bouthiette, L. Xiao, Marja M. Hurley
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8f4a75c58d1466393656753d15ebd3e
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spelling	oai:doaj.org-article:f8f4a75c58d1466393656753d15ebd3e2021-12-02T15:00:40ZGait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice10.1038/s41598-021-90565-02045-2322https://doaj.org/article/f8f4a75c58d1466393656753d15ebd3e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90565-0https://doaj.org/toc/2045-2322Abstract Fibroblast growth factor 2 (FGF2) is important in musculoskeletal homeostasis, therefore the impact of reduction or Fgf2 knockout on skeletal muscle function and phenotype was determined. Gait analysis as well as muscle strength testing in young and old WT and Fgf2KO demonstrated age-related gait disturbances and reduction in muscle strength that were exacerbated in the KO condition. Fgf2 mRNA and protein were significantly decreased in skeletal muscle of old WT compared with young WT. Muscle fiber cross-sectional area was significantly reduced with increased fibrosis and inflammatory infiltrates in old WT and Fgf2KO vs. young WT. Inflammatory cells were further significantly increased in old Fgf2KO compared with old WT. Lipid-related genes and intramuscular fat was increased in old WT and old Fgf2KO with a further increase in fibro-adipocytes in old Fgf2KO compared with old WT. Impaired FGF signaling including Increased β-Klotho, Fgf21 mRNA, FGF21 protein, phosphorylated FGF receptors 1 and 3, was observed in old WT and old Fgf2KO. MAPK/ ERK1/2 was significantly increased in young and old Fgf2KO. We conclude that Fgf2KO, age-related decreased FGF2 in WT mice, and increased FGF21 in the setting of impaired Fgf2 expression likely contribute to impaired skeletal muscle function and sarcopenia in mice.C. Homer-BouthietteL. XiaoMarja M. HurleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q C. Homer-Bouthiette L. Xiao Marja M. Hurley Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice
description	Abstract Fibroblast growth factor 2 (FGF2) is important in musculoskeletal homeostasis, therefore the impact of reduction or Fgf2 knockout on skeletal muscle function and phenotype was determined. Gait analysis as well as muscle strength testing in young and old WT and Fgf2KO demonstrated age-related gait disturbances and reduction in muscle strength that were exacerbated in the KO condition. Fgf2 mRNA and protein were significantly decreased in skeletal muscle of old WT compared with young WT. Muscle fiber cross-sectional area was significantly reduced with increased fibrosis and inflammatory infiltrates in old WT and Fgf2KO vs. young WT. Inflammatory cells were further significantly increased in old Fgf2KO compared with old WT. Lipid-related genes and intramuscular fat was increased in old WT and old Fgf2KO with a further increase in fibro-adipocytes in old Fgf2KO compared with old WT. Impaired FGF signaling including Increased β-Klotho, Fgf21 mRNA, FGF21 protein, phosphorylated FGF receptors 1 and 3, was observed in old WT and old Fgf2KO. MAPK/ ERK1/2 was significantly increased in young and old Fgf2KO. We conclude that Fgf2KO, age-related decreased FGF2 in WT mice, and increased FGF21 in the setting of impaired Fgf2 expression likely contribute to impaired skeletal muscle function and sarcopenia in mice.
format	article
author	C. Homer-Bouthiette L. Xiao Marja M. Hurley
author_facet	C. Homer-Bouthiette L. Xiao Marja M. Hurley
author_sort	C. Homer-Bouthiette
title	Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice
title_short	Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice
title_full	Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice
title_fullStr	Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice
title_full_unstemmed	Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice
title_sort	gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f8f4a75c58d1466393656753d15ebd3e
work_keys_str_mv	AT chomerbouthiette gaitdisturbancesandmuscledysfunctioninfibroblastgrowthfactor2knockoutmice AT lxiao gaitdisturbancesandmuscledysfunctioninfibroblastgrowthfactor2knockoutmice AT marjamhurley gaitdisturbancesandmuscledysfunctioninfibroblastgrowthfactor2knockoutmice
_version_	1718389177552207872

Gait disturbances and muscle dysfunction in fibroblast growth factor 2 knockout mice

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