Multi-scale molecular photoacoustic tomography of gene expression.

Photoacoustic tomography (PAT) is a molecular imaging technology. Unlike conventional reporter gene imaging, which is usually based on fluorescence, photoacoustic reporter gene imaging relies only on optical absorption. This work demonstrates several key merits of PAT using lacZ, one of the most wid...

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Autores principales:	Xin Cai, Li Li, Arie Krumholz, Zijian Guo, Todd N Erpelding, Chi Zhang, Yu Zhang, Younan Xia, Lihong V Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f8f9669c8147451a9f16df3a4c5cc59e
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spelling	oai:doaj.org-article:f8f9669c8147451a9f16df3a4c5cc59e2021-11-18T07:07:29ZMulti-scale molecular photoacoustic tomography of gene expression.1932-620310.1371/journal.pone.0043999https://doaj.org/article/f8f9669c8147451a9f16df3a4c5cc59e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22952846/?tool=EBIhttps://doaj.org/toc/1932-6203Photoacoustic tomography (PAT) is a molecular imaging technology. Unlike conventional reporter gene imaging, which is usually based on fluorescence, photoacoustic reporter gene imaging relies only on optical absorption. This work demonstrates several key merits of PAT using lacZ, one of the most widely used reporter genes in biology. We show that the expression of lacZ can be imaged by PAT as deep as 5.0 cm in biological tissue, with resolutions of ∼1.0 mm and ∼0.4 mm in the lateral and axial directions, respectively. We further demonstrate non-invasive, simultaneous imaging of a lacZ-expressing tumor and its surrounding microvasculature in vivo by dual-wavelength acoustic-resolution photoacoustic microscopy (AR-PAM), with a lateral resolution of 45 µm and an axial resolution of 15 µm. Finally, using optical-resolution photoacoustic microscopy (OR-PAM), we show intra-cellular localization of lacZ expression, with a lateral resolution of a fraction of a micron. These results suggest that PAT is a complementary tool to conventional optical fluorescence imaging of reporter genes for linking biological studies from the microscopic to the macroscopic scales.Xin CaiLi LiArie KrumholzZijian GuoTodd N ErpeldingChi ZhangYu ZhangYounan XiaLihong V WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e43999 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xin Cai Li Li Arie Krumholz Zijian Guo Todd N Erpelding Chi Zhang Yu Zhang Younan Xia Lihong V Wang Multi-scale molecular photoacoustic tomography of gene expression.
description	Photoacoustic tomography (PAT) is a molecular imaging technology. Unlike conventional reporter gene imaging, which is usually based on fluorescence, photoacoustic reporter gene imaging relies only on optical absorption. This work demonstrates several key merits of PAT using lacZ, one of the most widely used reporter genes in biology. We show that the expression of lacZ can be imaged by PAT as deep as 5.0 cm in biological tissue, with resolutions of ∼1.0 mm and ∼0.4 mm in the lateral and axial directions, respectively. We further demonstrate non-invasive, simultaneous imaging of a lacZ-expressing tumor and its surrounding microvasculature in vivo by dual-wavelength acoustic-resolution photoacoustic microscopy (AR-PAM), with a lateral resolution of 45 µm and an axial resolution of 15 µm. Finally, using optical-resolution photoacoustic microscopy (OR-PAM), we show intra-cellular localization of lacZ expression, with a lateral resolution of a fraction of a micron. These results suggest that PAT is a complementary tool to conventional optical fluorescence imaging of reporter genes for linking biological studies from the microscopic to the macroscopic scales.
format	article
author	Xin Cai Li Li Arie Krumholz Zijian Guo Todd N Erpelding Chi Zhang Yu Zhang Younan Xia Lihong V Wang
author_facet	Xin Cai Li Li Arie Krumholz Zijian Guo Todd N Erpelding Chi Zhang Yu Zhang Younan Xia Lihong V Wang
author_sort	Xin Cai
title	Multi-scale molecular photoacoustic tomography of gene expression.
title_short	Multi-scale molecular photoacoustic tomography of gene expression.
title_full	Multi-scale molecular photoacoustic tomography of gene expression.
title_fullStr	Multi-scale molecular photoacoustic tomography of gene expression.
title_full_unstemmed	Multi-scale molecular photoacoustic tomography of gene expression.
title_sort	multi-scale molecular photoacoustic tomography of gene expression.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f8f9669c8147451a9f16df3a4c5cc59e
work_keys_str_mv	AT xincai multiscalemolecularphotoacoustictomographyofgeneexpression AT lili multiscalemolecularphotoacoustictomographyofgeneexpression AT ariekrumholz multiscalemolecularphotoacoustictomographyofgeneexpression AT zijianguo multiscalemolecularphotoacoustictomographyofgeneexpression AT toddnerpelding multiscalemolecularphotoacoustictomographyofgeneexpression AT chizhang multiscalemolecularphotoacoustictomographyofgeneexpression AT yuzhang multiscalemolecularphotoacoustictomographyofgeneexpression AT younanxia multiscalemolecularphotoacoustictomographyofgeneexpression AT lihongvwang multiscalemolecularphotoacoustictomographyofgeneexpression
_version_	1718423954635358208

Multi-scale molecular photoacoustic tomography of gene expression.

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