Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier

Abstract Current neural interface technologies have serious limitations for advanced prosthetic and therapeutic applications due primarily to their lack of specificity in neural communication. An optogenetic approach has the potential to provide single cell/axon resolution in a minimally invasive ma...

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Autores principales:	Arjun K. Fontaine, Emily A. Gibson, John H. Caldwell, Richard F. Weir
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9039b2e13e74b81b9ba19b83d44cb3f
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spelling	oai:doaj.org-article:f9039b2e13e74b81b9ba19b83d44cb3f2021-12-02T12:32:57ZOptical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier10.1038/s41598-017-03541-y2045-2322https://doaj.org/article/f9039b2e13e74b81b9ba19b83d44cb3f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03541-yhttps://doaj.org/toc/2045-2322Abstract Current neural interface technologies have serious limitations for advanced prosthetic and therapeutic applications due primarily to their lack of specificity in neural communication. An optogenetic approach has the potential to provide single cell/axon resolution in a minimally invasive manner by optical interrogation of light-sensitive reporters and actuators. Given the aim of reading neural activity in the peripheral nervous system, this work has investigated an activity-dependent signaling mechanism in the peripheral nerve. We demonstrate action potential evoked calcium signals in mammalian tibial nerve axons using an in vitro mouse model with a dextran-conjugated fluorescent calcium indicator. Spatial and temporal dynamics of the signal are presented, including characterization of frequency-modulated amplitude. Pharmacological experiments implicate T-type CaV channels and sodium-calcium exchanger (NCX) as predominant mechanisms of calcium influx. This work shows the potential of using calcium-associated optical signals for neural activity read-out in peripheral nerve axons.Arjun K. FontaineEmily A. GibsonJohn H. CaldwellRichard F. WeirNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Arjun K. Fontaine Emily A. Gibson John H. Caldwell Richard F. Weir Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier
description	Abstract Current neural interface technologies have serious limitations for advanced prosthetic and therapeutic applications due primarily to their lack of specificity in neural communication. An optogenetic approach has the potential to provide single cell/axon resolution in a minimally invasive manner by optical interrogation of light-sensitive reporters and actuators. Given the aim of reading neural activity in the peripheral nervous system, this work has investigated an activity-dependent signaling mechanism in the peripheral nerve. We demonstrate action potential evoked calcium signals in mammalian tibial nerve axons using an in vitro mouse model with a dextran-conjugated fluorescent calcium indicator. Spatial and temporal dynamics of the signal are presented, including characterization of frequency-modulated amplitude. Pharmacological experiments implicate T-type CaV channels and sodium-calcium exchanger (NCX) as predominant mechanisms of calcium influx. This work shows the potential of using calcium-associated optical signals for neural activity read-out in peripheral nerve axons.
format	article
author	Arjun K. Fontaine Emily A. Gibson John H. Caldwell Richard F. Weir
author_facet	Arjun K. Fontaine Emily A. Gibson John H. Caldwell Richard F. Weir
author_sort	Arjun K. Fontaine
title	Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier
title_short	Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier
title_full	Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier
title_fullStr	Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier
title_full_unstemmed	Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier
title_sort	optical read-out of neural activity in mammalian peripheral axons: calcium signaling at nodes of ranvier
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f9039b2e13e74b81b9ba19b83d44cb3f
work_keys_str_mv	AT arjunkfontaine opticalreadoutofneuralactivityinmammalianperipheralaxonscalciumsignalingatnodesofranvier AT emilyagibson opticalreadoutofneuralactivityinmammalianperipheralaxonscalciumsignalingatnodesofranvier AT johnhcaldwell opticalreadoutofneuralactivityinmammalianperipheralaxonscalciumsignalingatnodesofranvier AT richardfweir opticalreadoutofneuralactivityinmammalianperipheralaxonscalciumsignalingatnodesofranvier
_version_	1718393955831250944

Optical Read-out of Neural Activity in Mammalian Peripheral Axons: Calcium Signaling at Nodes of Ranvier

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