A rapid test for soy aeroallergens exposure assessment.

<h4>Background</h4>Determining soy aeroallergens levels is extremely important in the assessment of health risks due to these airborne substances. Currently, soy aeroallergens exposure in the environment is monitored using enzyme immunoassays (EIA) which must be evaluated in a specialize...

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Autores principales:	Daniel Alvarez-Simon, María-Jesús Cruz, María-Dolores Untoria, Xavier Muñoz, Joan R Villalbí, Ferran Morell, Susana Gómez-Ollés
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f905103380bd411c8ed9ce0be4df486e
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spelling	oai:doaj.org-article:f905103380bd411c8ed9ce0be4df486e2021-11-18T08:32:46ZA rapid test for soy aeroallergens exposure assessment.1932-620310.1371/journal.pone.0088676https://doaj.org/article/f905103380bd411c8ed9ce0be4df486e2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24533134/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Determining soy aeroallergens levels is extremely important in the assessment of health risks due to these airborne substances. Currently, soy aeroallergens exposure in the environment is monitored using enzyme immunoassays (EIA) which must be evaluated in a specialized laboratory by skilled personnel.<h4>Objective</h4>To describe the development and performance of a rapid immunochromatography assay for the detection of soy aeroallergens in environmental samples.<h4>Methods</h4>A test strip using gold labeled anti-soy hull low molecular weight extract (SHLMWE) antibody for the rapid detection of soy aeroallergens in environmental samples was developed. One hundred nineteen airborne samples were analysed in parallel by the strip assay and the anti-SHLMWE sandwich EIA. The assay results were visually analysed by three independent observers who ranked samples as: -, + or ++. Strips were also scanned and analysed by densitometry.<h4>Results</h4>The rapid test detected a range of concentrations from 6.25 to 25 ng/mL. Agreement in strip assay interpretations between evaluators was substantial (Kappa = 0.63; CI 0.544-0.715). Visual interpretation also gave a good concordance with EIA results, with sensitivity ranging from 77.3 to 100 and specificity from 65 to 83.5 depending on the observer. Furthermore, a strong correlation was observed between densitometry results of strip assay and EIA determinations.<h4>Conclusions</h4>The strip assay developed is rapid, simple, and sensitive and does not require expensive equipment or specific skills. It has considerable potential in the environmental monitoring field for screening soy aeroallergens levels in port cities where allergen measurements are not currently performed. Due to its simplicity, the test will improve the management of soy allergic patients by controlling environmental allergen exposure without the need for apparatus or skilled personnel.Daniel Alvarez-SimonMaría-Jesús CruzMaría-Dolores UntoriaXavier MuñozJoan R VillalbíFerran MorellSusana Gómez-OllésPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 2, p e88676 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Daniel Alvarez-Simon María-Jesús Cruz María-Dolores Untoria Xavier Muñoz Joan R Villalbí Ferran Morell Susana Gómez-Ollés A rapid test for soy aeroallergens exposure assessment.
description	<h4>Background</h4>Determining soy aeroallergens levels is extremely important in the assessment of health risks due to these airborne substances. Currently, soy aeroallergens exposure in the environment is monitored using enzyme immunoassays (EIA) which must be evaluated in a specialized laboratory by skilled personnel.<h4>Objective</h4>To describe the development and performance of a rapid immunochromatography assay for the detection of soy aeroallergens in environmental samples.<h4>Methods</h4>A test strip using gold labeled anti-soy hull low molecular weight extract (SHLMWE) antibody for the rapid detection of soy aeroallergens in environmental samples was developed. One hundred nineteen airborne samples were analysed in parallel by the strip assay and the anti-SHLMWE sandwich EIA. The assay results were visually analysed by three independent observers who ranked samples as: -, + or ++. Strips were also scanned and analysed by densitometry.<h4>Results</h4>The rapid test detected a range of concentrations from 6.25 to 25 ng/mL. Agreement in strip assay interpretations between evaluators was substantial (Kappa = 0.63; CI 0.544-0.715). Visual interpretation also gave a good concordance with EIA results, with sensitivity ranging from 77.3 to 100 and specificity from 65 to 83.5 depending on the observer. Furthermore, a strong correlation was observed between densitometry results of strip assay and EIA determinations.<h4>Conclusions</h4>The strip assay developed is rapid, simple, and sensitive and does not require expensive equipment or specific skills. It has considerable potential in the environmental monitoring field for screening soy aeroallergens levels in port cities where allergen measurements are not currently performed. Due to its simplicity, the test will improve the management of soy allergic patients by controlling environmental allergen exposure without the need for apparatus or skilled personnel.
format	article
author	Daniel Alvarez-Simon María-Jesús Cruz María-Dolores Untoria Xavier Muñoz Joan R Villalbí Ferran Morell Susana Gómez-Ollés
author_facet	Daniel Alvarez-Simon María-Jesús Cruz María-Dolores Untoria Xavier Muñoz Joan R Villalbí Ferran Morell Susana Gómez-Ollés
author_sort	Daniel Alvarez-Simon
title	A rapid test for soy aeroallergens exposure assessment.
title_short	A rapid test for soy aeroallergens exposure assessment.
title_full	A rapid test for soy aeroallergens exposure assessment.
title_fullStr	A rapid test for soy aeroallergens exposure assessment.
title_full_unstemmed	A rapid test for soy aeroallergens exposure assessment.
title_sort	rapid test for soy aeroallergens exposure assessment.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f905103380bd411c8ed9ce0be4df486e
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A rapid test for soy aeroallergens exposure assessment.

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