MPS1 is involved in the HPV16-E7-mediated centrosomes amplification
Abstract Background It has been reported that the oncoprotein E7 from human papillomavirus type 16 (HPV16-E7) can induce the excessive synthesis of centrosomes through the increase in the expression of PLK4, which is a transcriptional target of E2F1. On the other hand, it has been reported that incr...
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2021
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oai:doaj.org-article:f9089074b74a48a2b5cc0487f933ceb02021-11-07T12:12:55ZMPS1 is involved in the HPV16-E7-mediated centrosomes amplification10.1186/s13008-021-00074-91747-1028https://doaj.org/article/f9089074b74a48a2b5cc0487f933ceb02021-11-01T00:00:00Zhttps://doi.org/10.1186/s13008-021-00074-9https://doaj.org/toc/1747-1028Abstract Background It has been reported that the oncoprotein E7 from human papillomavirus type 16 (HPV16-E7) can induce the excessive synthesis of centrosomes through the increase in the expression of PLK4, which is a transcriptional target of E2F1. On the other hand, it has been reported that increasing MPS1 protein stability can also generate an excessive synthesis of centrosomes. In this work, we analyzed the possible role of MPS1 in the amplification of centrosomes mediated by HPV16-E7. Results Employing qRT-PCR, Western Blot, and Immunofluorescence techniques, we found that E7 induces an increase in the MPS1 transcript and protein levels in the U2OS cell line, as well as protein stabilization. Besides, we observed that inhibiting the expression of MPS1 in E7 protein-expressing cells leads to a significant reduction in the number of centrosomes. Conclusions These results indicate that the presence of the MPS1 protein is necessary for E7 protein to increase the number of centrosomes, and possible implications are discussed.Yair Alfaro-MoraGuadalupe Domínguez-GómezRodrigo E. Cáceres-GutiérrezLaura Tolentino-GarcíaLuis A. HerreraClementina Castro-HernándezRosa María Bermúdez-CruzJosé Díaz-ChávezBMCarticleCentrosomeMPS1PLK4HPV16-E7Neoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282CytologyQH573-671ENCell Division, Vol 16, Iss 1, Pp 1-13 (2021) |
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DOAJ |
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DOAJ |
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EN |
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Centrosome MPS1 PLK4 HPV16-E7 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 |
| spellingShingle |
Centrosome MPS1 PLK4 HPV16-E7 Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Cytology QH573-671 Yair Alfaro-Mora Guadalupe Domínguez-Gómez Rodrigo E. Cáceres-Gutiérrez Laura Tolentino-García Luis A. Herrera Clementina Castro-Hernández Rosa María Bermúdez-Cruz José Díaz-Chávez MPS1 is involved in the HPV16-E7-mediated centrosomes amplification |
| description |
Abstract Background It has been reported that the oncoprotein E7 from human papillomavirus type 16 (HPV16-E7) can induce the excessive synthesis of centrosomes through the increase in the expression of PLK4, which is a transcriptional target of E2F1. On the other hand, it has been reported that increasing MPS1 protein stability can also generate an excessive synthesis of centrosomes. In this work, we analyzed the possible role of MPS1 in the amplification of centrosomes mediated by HPV16-E7. Results Employing qRT-PCR, Western Blot, and Immunofluorescence techniques, we found that E7 induces an increase in the MPS1 transcript and protein levels in the U2OS cell line, as well as protein stabilization. Besides, we observed that inhibiting the expression of MPS1 in E7 protein-expressing cells leads to a significant reduction in the number of centrosomes. Conclusions These results indicate that the presence of the MPS1 protein is necessary for E7 protein to increase the number of centrosomes, and possible implications are discussed. |
| format |
article |
| author |
Yair Alfaro-Mora Guadalupe Domínguez-Gómez Rodrigo E. Cáceres-Gutiérrez Laura Tolentino-García Luis A. Herrera Clementina Castro-Hernández Rosa María Bermúdez-Cruz José Díaz-Chávez |
| author_facet |
Yair Alfaro-Mora Guadalupe Domínguez-Gómez Rodrigo E. Cáceres-Gutiérrez Laura Tolentino-García Luis A. Herrera Clementina Castro-Hernández Rosa María Bermúdez-Cruz José Díaz-Chávez |
| author_sort |
Yair Alfaro-Mora |
| title |
MPS1 is involved in the HPV16-E7-mediated centrosomes amplification |
| title_short |
MPS1 is involved in the HPV16-E7-mediated centrosomes amplification |
| title_full |
MPS1 is involved in the HPV16-E7-mediated centrosomes amplification |
| title_fullStr |
MPS1 is involved in the HPV16-E7-mediated centrosomes amplification |
| title_full_unstemmed |
MPS1 is involved in the HPV16-E7-mediated centrosomes amplification |
| title_sort |
mps1 is involved in the hpv16-e7-mediated centrosomes amplification |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/f9089074b74a48a2b5cc0487f933ceb0 |
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