Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.

Faithful inheritance of mitochondrial DNA (mtDNA) is crucial for cellular respiration/oxidative phosphorylation and mitochondrial membrane potential. However, how mtDNA is transmitted to progeny is not fully understood. We utilized hypersuppressive mtDNA, a class of respiratory deficient Saccharomyc...

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Autores principales:	Daniel Corbi, Angelika Amon
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Genetics QH426-470
Acceso en línea:	https://doaj.org/article/f909f2d7178f451eac63b3dc87e1101b
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spelling	oai:doaj.org-article:f909f2d7178f451eac63b3dc87e1101b2021-12-02T20:03:31ZDecreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.1553-73901553-740410.1371/journal.pgen.1009808https://doaj.org/article/f909f2d7178f451eac63b3dc87e1101b2021-10-01T00:00:00Zhttps://doi.org/10.1371/journal.pgen.1009808https://doaj.org/toc/1553-7390https://doaj.org/toc/1553-7404Faithful inheritance of mitochondrial DNA (mtDNA) is crucial for cellular respiration/oxidative phosphorylation and mitochondrial membrane potential. However, how mtDNA is transmitted to progeny is not fully understood. We utilized hypersuppressive mtDNA, a class of respiratory deficient Saccharomyces cerevisiae mtDNA that is preferentially inherited over wild-type mtDNA (rho+), to uncover the factors governing mtDNA inheritance. We found that some regions of rho+ mtDNA persisted while others were lost after a specific hypersuppressive takeover indicating that hypersuppressive preferential inheritance may partially be due to active destruction of rho+ mtDNA. From a multicopy suppression screen, we found that overexpression of putative mitochondrial RNA exonuclease PET127 reduced biased inheritance of a subset of hypersuppressive genomes. This suppression required PET127 binding to the mitochondrial RNA polymerase RPO41 but not PET127 exonuclease activity. A temperature-sensitive allele of RPO41 improved rho+ mtDNA inheritance over a specific hypersuppressive mtDNA at semi-permissive temperatures revealing a previously unknown role for rho+ transcription in promoting hypersuppressive mtDNA inheritance.Daniel CorbiAngelika AmonPublic Library of Science (PLoS)articleGeneticsQH426-470ENPLoS Genetics, Vol 17, Iss 10, p e1009808 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Genetics QH426-470
spellingShingle	Genetics QH426-470 Daniel Corbi Angelika Amon Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.
description	Faithful inheritance of mitochondrial DNA (mtDNA) is crucial for cellular respiration/oxidative phosphorylation and mitochondrial membrane potential. However, how mtDNA is transmitted to progeny is not fully understood. We utilized hypersuppressive mtDNA, a class of respiratory deficient Saccharomyces cerevisiae mtDNA that is preferentially inherited over wild-type mtDNA (rho+), to uncover the factors governing mtDNA inheritance. We found that some regions of rho+ mtDNA persisted while others were lost after a specific hypersuppressive takeover indicating that hypersuppressive preferential inheritance may partially be due to active destruction of rho+ mtDNA. From a multicopy suppression screen, we found that overexpression of putative mitochondrial RNA exonuclease PET127 reduced biased inheritance of a subset of hypersuppressive genomes. This suppression required PET127 binding to the mitochondrial RNA polymerase RPO41 but not PET127 exonuclease activity. A temperature-sensitive allele of RPO41 improved rho+ mtDNA inheritance over a specific hypersuppressive mtDNA at semi-permissive temperatures revealing a previously unknown role for rho+ transcription in promoting hypersuppressive mtDNA inheritance.
format	article
author	Daniel Corbi Angelika Amon
author_facet	Daniel Corbi Angelika Amon
author_sort	Daniel Corbi
title	Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.
title_short	Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.
title_full	Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.
title_fullStr	Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.
title_full_unstemmed	Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.
title_sort	decreasing mitochondrial rna polymerase activity reverses biased inheritance of hypersuppressive mtdna.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/f909f2d7178f451eac63b3dc87e1101b
work_keys_str_mv	AT danielcorbi decreasingmitochondrialrnapolymeraseactivityreversesbiasedinheritanceofhypersuppressivemtdna AT angelikaamon decreasingmitochondrialrnapolymeraseactivityreversesbiasedinheritanceofhypersuppressivemtdna
_version_	1718375698714853376

Decreasing mitochondrial RNA polymerase activity reverses biased inheritance of hypersuppressive mtDNA.

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