Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle

Abstract Epigenetic changes induced in the early developmental stages by the surrounding environment can have not only short-term but also long-term consequences throughout life. This concept constitutes the “Developmental Origins of Health and Disease” (DOHaD) hypothesis and encompasses the possibi...

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Autores principales:	Mao Ishibashi, Shuntaro Ikeda, Naojiro Minami
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f90c2bd74f5f467ba55a9e4b9c67b51b
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spelling	oai:doaj.org-article:f90c2bd74f5f467ba55a9e4b9c67b51b2021-12-02T14:26:13ZComparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle10.1038/s41598-021-87683-02045-2322https://doaj.org/article/f90c2bd74f5f467ba55a9e4b9c67b51b2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87683-0https://doaj.org/toc/2045-2322Abstract Epigenetic changes induced in the early developmental stages by the surrounding environment can have not only short-term but also long-term consequences throughout life. This concept constitutes the “Developmental Origins of Health and Disease” (DOHaD) hypothesis and encompasses the possibility of controlling livestock health and diseases by epigenetic regulation during early development. As a preliminary step for examining changes of epigenetic modifications in early embryos and their long-lasting effects in fully differentiated somatic tissues, we aimed to obtain high-throughput genome-wide histone H3 lysine 4 trimethylation (H3K4me3) profiles of bovine blastocysts and to compare these data with those from adult somatic tissues in order to extract common and typical features between these tissues in terms of H3K4me3 modifications. Bovine blastocysts were produced in vitro and subjected to chromatin immunoprecipitation-sequencing analysis of H3K4me3. Comparative analysis of the blastocyst-derived H3K4me3 profile with publicly available data from adult liver and muscle tissues revealed that the blastocyst profile could be used as a “sieve” to extract somatic tissue-specific modifications in genes closely related to tissue-specific functions. Furthermore, principal component analysis of the level of common modifications between blastocysts and somatic tissues in meat production-related and imprinted genes well characterized inter- and intra-tissue differences. The results of this study produced a referential genome-wide H3K4me3 profile of bovine blastocysts within the limits of their in vitro source and revealed its common and typical features in relation to the profiles of adult tissues.Mao IshibashiShuntaro IkedaNaojiro MinamiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mao Ishibashi Shuntaro Ikeda Naojiro Minami Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle
description	Abstract Epigenetic changes induced in the early developmental stages by the surrounding environment can have not only short-term but also long-term consequences throughout life. This concept constitutes the “Developmental Origins of Health and Disease” (DOHaD) hypothesis and encompasses the possibility of controlling livestock health and diseases by epigenetic regulation during early development. As a preliminary step for examining changes of epigenetic modifications in early embryos and their long-lasting effects in fully differentiated somatic tissues, we aimed to obtain high-throughput genome-wide histone H3 lysine 4 trimethylation (H3K4me3) profiles of bovine blastocysts and to compare these data with those from adult somatic tissues in order to extract common and typical features between these tissues in terms of H3K4me3 modifications. Bovine blastocysts were produced in vitro and subjected to chromatin immunoprecipitation-sequencing analysis of H3K4me3. Comparative analysis of the blastocyst-derived H3K4me3 profile with publicly available data from adult liver and muscle tissues revealed that the blastocyst profile could be used as a “sieve” to extract somatic tissue-specific modifications in genes closely related to tissue-specific functions. Furthermore, principal component analysis of the level of common modifications between blastocysts and somatic tissues in meat production-related and imprinted genes well characterized inter- and intra-tissue differences. The results of this study produced a referential genome-wide H3K4me3 profile of bovine blastocysts within the limits of their in vitro source and revealed its common and typical features in relation to the profiles of adult tissues.
format	article
author	Mao Ishibashi Shuntaro Ikeda Naojiro Minami
author_facet	Mao Ishibashi Shuntaro Ikeda Naojiro Minami
author_sort	Mao Ishibashi
title	Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle
title_short	Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle
title_full	Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle
title_fullStr	Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle
title_full_unstemmed	Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle
title_sort	comparative analysis of histone h3k4me3 modifications between blastocysts and somatic tissues in cattle
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f90c2bd74f5f467ba55a9e4b9c67b51b
work_keys_str_mv	AT maoishibashi comparativeanalysisofhistoneh3k4me3modificationsbetweenblastocystsandsomatictissuesincattle AT shuntaroikeda comparativeanalysisofhistoneh3k4me3modificationsbetweenblastocystsandsomatictissuesincattle AT naojirominami comparativeanalysisofhistoneh3k4me3modificationsbetweenblastocystsandsomatictissuesincattle
_version_	1718391406102315008

Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle

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