Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan

A biofungicide is a natural product that can be derived from various sources such as, among others, microorganisms, higher plants, animal products, phytochemicals, semiochemicals, and antagonist microorganisms. One of the most important approaches for the production of biofungicides is the combinati...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ariadna Berenice Trejo-Raya, Víctor Manuel Rodríguez-Romero, Silvia Bautista-Baños, Francisco Roberto Quiroz-Figueroa, Ramón Villanueva-Arce, Enrique Durán-Páramo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Alternaria altenata</i> <i>Fusarium solani</i> siderophores antagonist biofungicide Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/f90cb5eb197949918207d2b978e99aa1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f90cb5eb197949918207d2b978e99aa1
record_format	dspace
spelling	oai:doaj.org-article:f90cb5eb197949918207d2b978e99aa12021-11-11T18:23:12ZEffective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan10.3390/molecules262163591420-3049https://doaj.org/article/f90cb5eb197949918207d2b978e99aa12021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6359https://doaj.org/toc/1420-3049A biofungicide is a natural product that can be derived from various sources such as, among others, microorganisms, higher plants, animal products, phytochemicals, semiochemicals, and antagonist microorganisms. One of the most important approaches for the production of biofungicides is the combination of biocontrol agents. This study showed the inhibition growth of <i>Alternaria alternata</i> and <i>Fusarium solani</i> treated with cell-free extracts of <i>P. fluorescens</i>. Using thin-layer chromatography and plate assays it was also demonstrated that the cell-free extracts of <i>P. fluorescens</i> contained siderophores and derivates of 4-diacetylphloroglucinol and phenazine. Moreover, the combination of cell-free extracts of <i>P. fluorescens</i> and chitosan [50–1.5% (<i>v</i>/<i>v</i>)] had a synergistic effect since they notably inhibited the mycelial growth of <i>A. altenata</i> and <i>F. solani</i>. Various morphological alterations to the mycelia and conidia of the treated fungi as a result of this combination were also observed. The present study could be a starting point to control other fungal phytopathogens using different cell-free extracts and chitosan as biocontrol agents.Ariadna Berenice Trejo-RayaVíctor Manuel Rodríguez-RomeroSilvia Bautista-BañosFrancisco Roberto Quiroz-FigueroaRamón Villanueva-ArceEnrique Durán-PáramoMDPI AGarticle<i>Alternaria altenata</i><i>Fusarium solani</i>siderophoresantagonistbiofungicideOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6359, p 6359 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Alternaria altenata</i> <i>Fusarium solani</i> siderophores antagonist biofungicide Organic chemistry QD241-441
spellingShingle	<i>Alternaria altenata</i> <i>Fusarium solani</i> siderophores antagonist biofungicide Organic chemistry QD241-441 Ariadna Berenice Trejo-Raya Víctor Manuel Rodríguez-Romero Silvia Bautista-Baños Francisco Roberto Quiroz-Figueroa Ramón Villanueva-Arce Enrique Durán-Páramo Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan
description	A biofungicide is a natural product that can be derived from various sources such as, among others, microorganisms, higher plants, animal products, phytochemicals, semiochemicals, and antagonist microorganisms. One of the most important approaches for the production of biofungicides is the combination of biocontrol agents. This study showed the inhibition growth of <i>Alternaria alternata</i> and <i>Fusarium solani</i> treated with cell-free extracts of <i>P. fluorescens</i>. Using thin-layer chromatography and plate assays it was also demonstrated that the cell-free extracts of <i>P. fluorescens</i> contained siderophores and derivates of 4-diacetylphloroglucinol and phenazine. Moreover, the combination of cell-free extracts of <i>P. fluorescens</i> and chitosan [50–1.5% (<i>v</i>/<i>v</i>)] had a synergistic effect since they notably inhibited the mycelial growth of <i>A. altenata</i> and <i>F. solani</i>. Various morphological alterations to the mycelia and conidia of the treated fungi as a result of this combination were also observed. The present study could be a starting point to control other fungal phytopathogens using different cell-free extracts and chitosan as biocontrol agents.
format	article
author	Ariadna Berenice Trejo-Raya Víctor Manuel Rodríguez-Romero Silvia Bautista-Baños Francisco Roberto Quiroz-Figueroa Ramón Villanueva-Arce Enrique Durán-Páramo
author_facet	Ariadna Berenice Trejo-Raya Víctor Manuel Rodríguez-Romero Silvia Bautista-Baños Francisco Roberto Quiroz-Figueroa Ramón Villanueva-Arce Enrique Durán-Páramo
author_sort	Ariadna Berenice Trejo-Raya
title	Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan
title_short	Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan
title_full	Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan
title_fullStr	Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan
title_full_unstemmed	Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan
title_sort	effective in vitro control of two phytopathogens of agricultural interest using cell-free extracts of <i>pseudomonas fluorescens</i> and chitosan
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f90cb5eb197949918207d2b978e99aa1
work_keys_str_mv	AT ariadnaberenicetrejoraya effectiveinvitrocontroloftwophytopathogensofagriculturalinterestusingcellfreeextractsofipseudomonasfluorescensiandchitosan AT victormanuelrodriguezromero effectiveinvitrocontroloftwophytopathogensofagriculturalinterestusingcellfreeextractsofipseudomonasfluorescensiandchitosan AT silviabautistabanos effectiveinvitrocontroloftwophytopathogensofagriculturalinterestusingcellfreeextractsofipseudomonasfluorescensiandchitosan AT franciscorobertoquirozfigueroa effectiveinvitrocontroloftwophytopathogensofagriculturalinterestusingcellfreeextractsofipseudomonasfluorescensiandchitosan AT ramonvillanuevaarce effectiveinvitrocontroloftwophytopathogensofagriculturalinterestusingcellfreeextractsofipseudomonasfluorescensiandchitosan AT enriqueduranparamo effectiveinvitrocontroloftwophytopathogensofagriculturalinterestusingcellfreeextractsofipseudomonasfluorescensiandchitosan
_version_	1718431859350700032

Effective In Vitro Control of Two Phytopathogens of Agricultural Interest Using Cell-Free Extracts of <i>Pseudomonas fluorescens</i> and Chitosan

Ejemplares similares