The cell surface proteome of human mesenchymal stromal cells.

<h4>Background</h4>Multipotent human mesenchymal stromal cells (hMSCs) are considered as promising biological tools for regenerative medicine. Their antibody-based isolation relies on the identification of reliable cell surface markers.<h4>Methodology/principal findings</h4>T...

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Autores principales:	Christian Niehage, Charlotte Steenblock, Theresia Pursche, Martin Bornhäuser, Denis Corbeil, Bernard Hoflack
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f90ed62786b7474ebfdad42cfe4fcd02
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spelling	oai:doaj.org-article:f90ed62786b7474ebfdad42cfe4fcd022021-11-18T06:53:11ZThe cell surface proteome of human mesenchymal stromal cells.1932-620310.1371/journal.pone.0020399https://doaj.org/article/f90ed62786b7474ebfdad42cfe4fcd022011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21637820/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Multipotent human mesenchymal stromal cells (hMSCs) are considered as promising biological tools for regenerative medicine. Their antibody-based isolation relies on the identification of reliable cell surface markers.<h4>Methodology/principal findings</h4>To obtain a comprehensive view of the cell surface proteome of bone marrow-derived hMSCs, we have developed an analytical pipeline relying on cell surface biotinylation of intact cells using cell impermeable, cleavable sulfo-NHS-SS-biotin to enrich the plasma membrane proteins and mass spectrometry for identification with extremely high confidence. Among the 888 proteins identified, we found ≈200 bona fide plasma membrane proteins including 33 cell adhesion molecules and 26 signaling receptors. In total 41 CD markers including 5 novel ones (CD97, CD112, CD239, CD276, and CD316) were identified. The CD markers are distributed homogenously within plastic-adherent hMSC populations and their expression is modulated during the process of adipogenesis or osteogenesis. Moreover, our in silico analysis revealed a significant difference between the cell surface proteome of hMSCs and that of human embryonic stem cells reported previously.<h4>Conclusions/significance</h4>Collectively, our analytical methods not only provide a basis for further studies of mechanisms maintaining the multipotency of hMSCs within their niches and triggering their differentiation after signaling, but also a toolbox for a refined antibody-based identification of hMSC populations from different tissues and their isolation for therapeutic intervention.Christian NiehageCharlotte SteenblockTheresia PurscheMartin BornhäuserDenis CorbeilBernard HoflackPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 5, p e20399 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Niehage Charlotte Steenblock Theresia Pursche Martin Bornhäuser Denis Corbeil Bernard Hoflack The cell surface proteome of human mesenchymal stromal cells.
description	<h4>Background</h4>Multipotent human mesenchymal stromal cells (hMSCs) are considered as promising biological tools for regenerative medicine. Their antibody-based isolation relies on the identification of reliable cell surface markers.<h4>Methodology/principal findings</h4>To obtain a comprehensive view of the cell surface proteome of bone marrow-derived hMSCs, we have developed an analytical pipeline relying on cell surface biotinylation of intact cells using cell impermeable, cleavable sulfo-NHS-SS-biotin to enrich the plasma membrane proteins and mass spectrometry for identification with extremely high confidence. Among the 888 proteins identified, we found ≈200 bona fide plasma membrane proteins including 33 cell adhesion molecules and 26 signaling receptors. In total 41 CD markers including 5 novel ones (CD97, CD112, CD239, CD276, and CD316) were identified. The CD markers are distributed homogenously within plastic-adherent hMSC populations and their expression is modulated during the process of adipogenesis or osteogenesis. Moreover, our in silico analysis revealed a significant difference between the cell surface proteome of hMSCs and that of human embryonic stem cells reported previously.<h4>Conclusions/significance</h4>Collectively, our analytical methods not only provide a basis for further studies of mechanisms maintaining the multipotency of hMSCs within their niches and triggering their differentiation after signaling, but also a toolbox for a refined antibody-based identification of hMSC populations from different tissues and their isolation for therapeutic intervention.
format	article
author	Christian Niehage Charlotte Steenblock Theresia Pursche Martin Bornhäuser Denis Corbeil Bernard Hoflack
author_facet	Christian Niehage Charlotte Steenblock Theresia Pursche Martin Bornhäuser Denis Corbeil Bernard Hoflack
author_sort	Christian Niehage
title	The cell surface proteome of human mesenchymal stromal cells.
title_short	The cell surface proteome of human mesenchymal stromal cells.
title_full	The cell surface proteome of human mesenchymal stromal cells.
title_fullStr	The cell surface proteome of human mesenchymal stromal cells.
title_full_unstemmed	The cell surface proteome of human mesenchymal stromal cells.
title_sort	cell surface proteome of human mesenchymal stromal cells.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/f90ed62786b7474ebfdad42cfe4fcd02
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The cell surface proteome of human mesenchymal stromal cells.

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