Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function

Abstract Photosensitization-based antimicrobial therapy (PAT) is an alternative therapy aimed at achieving bacterial inactivation. Researchers use various photosensitizers to achieve bacterial inactivation. However, the most widely used approach involves the use of photosensitizers dispersed in aque...

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Autores principales:	Bishakh Rout, Chi-Hsien Liu, Wei-Chi Wu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f90f66360bf44781a55caa4afff0cca2
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spelling	oai:doaj.org-article:f90f66360bf44781a55caa4afff0cca22021-12-02T12:30:12ZPhotosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function10.1038/s41598-017-07444-w2045-2322https://doaj.org/article/f90f66360bf44781a55caa4afff0cca22017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07444-whttps://doaj.org/toc/2045-2322Abstract Photosensitization-based antimicrobial therapy (PAT) is an alternative therapy aimed at achieving bacterial inactivation. Researchers use various photosensitizers to achieve bacterial inactivation. However, the most widely used approach involves the use of photosensitizers dispersed in aqueous solution, which could limit the effectiveness of photodynamic inactivation. Therefore, the approaches to encapsulate the photosensitizer in appropriate vehicles can enhance the delivery of the photosensitizer. Herein, Toluidine Blue O (TBO) was the photosensitizer, and lipid nanoparticles were used for its encapsulation. The lipid nanoparticle-based delivery system has been tailor-made for decreasing the average size and viscosity and increasing the formulation stability as well as the wettability of skin. Usage of an appropriate vehicle will also increase the cellular uptake of the photosensitizer into the bacterial cells, leading to the damage on cell membrane and genomic DNA. Evidence of effectiveness of the developed PAT on planktonic bacteria and biofilms was examined by fluorescence microscopy and scanning electron microscopy. Lipid nanoparticles protected the photosensitizer from aggregation and made the application easy on the skin as indicated in data of size distribution and contact angle. The use of lipid nanoparticles for encapsulating TBO could enhance photosensitization-based antimicrobial therapy as compared to the aqueous media for delivering photosensitizers.Bishakh RoutChi-Hsien LiuWei-Chi WuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bishakh Rout Chi-Hsien Liu Wei-Chi Wu Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function
description	Abstract Photosensitization-based antimicrobial therapy (PAT) is an alternative therapy aimed at achieving bacterial inactivation. Researchers use various photosensitizers to achieve bacterial inactivation. However, the most widely used approach involves the use of photosensitizers dispersed in aqueous solution, which could limit the effectiveness of photodynamic inactivation. Therefore, the approaches to encapsulate the photosensitizer in appropriate vehicles can enhance the delivery of the photosensitizer. Herein, Toluidine Blue O (TBO) was the photosensitizer, and lipid nanoparticles were used for its encapsulation. The lipid nanoparticle-based delivery system has been tailor-made for decreasing the average size and viscosity and increasing the formulation stability as well as the wettability of skin. Usage of an appropriate vehicle will also increase the cellular uptake of the photosensitizer into the bacterial cells, leading to the damage on cell membrane and genomic DNA. Evidence of effectiveness of the developed PAT on planktonic bacteria and biofilms was examined by fluorescence microscopy and scanning electron microscopy. Lipid nanoparticles protected the photosensitizer from aggregation and made the application easy on the skin as indicated in data of size distribution and contact angle. The use of lipid nanoparticles for encapsulating TBO could enhance photosensitization-based antimicrobial therapy as compared to the aqueous media for delivering photosensitizers.
format	article
author	Bishakh Rout Chi-Hsien Liu Wei-Chi Wu
author_facet	Bishakh Rout Chi-Hsien Liu Wei-Chi Wu
author_sort	Bishakh Rout
title	Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function
title_short	Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function
title_full	Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function
title_fullStr	Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function
title_full_unstemmed	Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function
title_sort	photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f90f66360bf44781a55caa4afff0cca2
work_keys_str_mv	AT bishakhrout photosensitizerinlipidnanoparticleananoscaledapproachtoantibacterialfunction AT chihsienliu photosensitizerinlipidnanoparticleananoscaledapproachtoantibacterialfunction AT weichiwu photosensitizerinlipidnanoparticleananoscaledapproachtoantibacterialfunction
_version_	1718394432698449920

Photosensitizer in lipid nanoparticle: a nano-scaled approach to antibacterial function

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