A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome

Topoisomerase 2 (Top2) is known to resolve DNA topological stress through double strand breaks (DSBs), yet Top2 inhibition has been reported to result in a significant amount of single-strand breaks (SSBs). Here the authors develop CC-seq—a method that allows direct mapping of both Top2-linked SSBs...

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Autores principales:	William H. Gittens, Dominic J. Johnson, Rachal M. Allison, Tim J. Cooper, Holly Thomas, Matthew J. Neale
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f91830fdb6fb441087a9822e5cdba14e
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spelling	oai:doaj.org-article:f91830fdb6fb441087a9822e5cdba14e2021-12-02T14:38:43ZA nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome10.1038/s41467-019-12802-52041-1723https://doaj.org/article/f91830fdb6fb441087a9822e5cdba14e2019-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-12802-5https://doaj.org/toc/2041-1723Topoisomerase 2 (Top2) is known to resolve DNA topological stress through double strand breaks (DSBs), yet Top2 inhibition has been reported to result in a significant amount of single-strand breaks (SSBs). Here the authors develop CC-seq—a method that allows direct mapping of both Top2-linked SSBs and DSBs—and reveal a significant impact of primary DNA sequence on Top2 directed cleavage.William H. GittensDominic J. JohnsonRachal M. AllisonTim J. CooperHolly ThomasMatthew J. NealeNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-16 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q William H. Gittens Dominic J. Johnson Rachal M. Allison Tim J. Cooper Holly Thomas Matthew J. Neale A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome
description	Topoisomerase 2 (Top2) is known to resolve DNA topological stress through double strand breaks (DSBs), yet Top2 inhibition has been reported to result in a significant amount of single-strand breaks (SSBs). Here the authors develop CC-seq—a method that allows direct mapping of both Top2-linked SSBs and DSBs—and reveal a significant impact of primary DNA sequence on Top2 directed cleavage.
format	article
author	William H. Gittens Dominic J. Johnson Rachal M. Allison Tim J. Cooper Holly Thomas Matthew J. Neale
author_facet	William H. Gittens Dominic J. Johnson Rachal M. Allison Tim J. Cooper Holly Thomas Matthew J. Neale
author_sort	William H. Gittens
title	A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome
title_short	A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome
title_full	A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome
title_fullStr	A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome
title_full_unstemmed	A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome
title_sort	nucleotide resolution map of top2-linked dna breaks in the yeast and human genome
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/f91830fdb6fb441087a9822e5cdba14e
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A nucleotide resolution map of Top2-linked DNA breaks in the yeast and human genome

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