Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production

Abstract Human induced pluripotent stem cells (hiPSCs) can be differentiated into chondrocyte-like cells. However, implantation of these cells is not without risk given that those transplanted cells may one day undergo ionizing radiation (IR) in patients who develop cancer. We aimed to evaluate the...

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Autores principales:	Ewelina Stelcer, Katarzyna Kulcenty, Marcin Rucinski, Marta Kruszyna-Mochalska, Agnieszka Skrobala, Agnieszka Sobecka, Karol Jopek, Wiktoria Maria Suchorska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f91a3d76fc2949ebac10e37da13f30e3
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spelling	oai:doaj.org-article:f91a3d76fc2949ebac10e37da13f30e32021-12-02T18:15:24ZIonizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production10.1038/s41598-021-86230-12045-2322https://doaj.org/article/f91a3d76fc2949ebac10e37da13f30e32021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-86230-1https://doaj.org/toc/2045-2322Abstract Human induced pluripotent stem cells (hiPSCs) can be differentiated into chondrocyte-like cells. However, implantation of these cells is not without risk given that those transplanted cells may one day undergo ionizing radiation (IR) in patients who develop cancer. We aimed to evaluate the effect of IR on chondrocyte-like cells differentiated from hiPSCs by determining their gene and microRNA expression profile and proteomic analysis. Chondrocyte-like cells differentiated from hiPSCs were placed in a purpose-designed phantom to model laryngeal cancer and irradiated with 1, 2, or 3 Gy. High-throughput analyses were performed to determine the gene and microRNA expression profile based on microarrays. The composition of the medium was also analyzed. The following essential biological processes were activated in these hiPSC-derived chondrocytes after IR: "apoptotic process", "cellular response to DNA damage stimulus", and "regulation of programmed cell death". These findings show the microRNAs that are primarily responsible for controlling the genes of the biological processes described above. We also detected changes in the secretion level of specific cytokines. This study demonstrates that IR activates DNA damage response mechanisms in differentiated cells and that the level of activation is a function of the radiation dose.Ewelina StelcerKatarzyna KulcentyMarcin RucinskiMarta Kruszyna-MochalskaAgnieszka SkrobalaAgnieszka SobeckaKarol JopekWiktoria Maria SuchorskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ewelina Stelcer Katarzyna Kulcenty Marcin Rucinski Marta Kruszyna-Mochalska Agnieszka Skrobala Agnieszka Sobecka Karol Jopek Wiktoria Maria Suchorska Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production
description	Abstract Human induced pluripotent stem cells (hiPSCs) can be differentiated into chondrocyte-like cells. However, implantation of these cells is not without risk given that those transplanted cells may one day undergo ionizing radiation (IR) in patients who develop cancer. We aimed to evaluate the effect of IR on chondrocyte-like cells differentiated from hiPSCs by determining their gene and microRNA expression profile and proteomic analysis. Chondrocyte-like cells differentiated from hiPSCs were placed in a purpose-designed phantom to model laryngeal cancer and irradiated with 1, 2, or 3 Gy. High-throughput analyses were performed to determine the gene and microRNA expression profile based on microarrays. The composition of the medium was also analyzed. The following essential biological processes were activated in these hiPSC-derived chondrocytes after IR: "apoptotic process", "cellular response to DNA damage stimulus", and "regulation of programmed cell death". These findings show the microRNAs that are primarily responsible for controlling the genes of the biological processes described above. We also detected changes in the secretion level of specific cytokines. This study demonstrates that IR activates DNA damage response mechanisms in differentiated cells and that the level of activation is a function of the radiation dose.
format	article
author	Ewelina Stelcer Katarzyna Kulcenty Marcin Rucinski Marta Kruszyna-Mochalska Agnieszka Skrobala Agnieszka Sobecka Karol Jopek Wiktoria Maria Suchorska
author_facet	Ewelina Stelcer Katarzyna Kulcenty Marcin Rucinski Marta Kruszyna-Mochalska Agnieszka Skrobala Agnieszka Sobecka Karol Jopek Wiktoria Maria Suchorska
author_sort	Ewelina Stelcer
title	Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production
title_short	Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production
title_full	Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production
title_fullStr	Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production
title_full_unstemmed	Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production
title_sort	ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microrna expression profiles and cytokine production
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f91a3d76fc2949ebac10e37da13f30e3
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Ionizing radiation exposure of stem cell-derived chondrocytes affects their gene and microRNA expression profiles and cytokine production

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