FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS

The aim of the work was to study the parameters of T-cells, humoral immunity and activity of NAD(P)- dependent dehydrogenases in lymphocytes from peripheral blood at young children with recurrent obstructive bronchitis (ROB). Patients and Methods: Fifty-four children at the age of 1-3 years with ROB...

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Autores principales:	L. M. Kurtasova, N. A. Shakina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2017
Materias:	bronchial obstruction bronchitis recurrent lymphocyte phenotype immunoglobulins metabolism Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/f91abe7107fe4639984b0184246d413c
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spelling	oai:doaj.org-article:f91abe7107fe4639984b0184246d413c2021-11-18T08:03:46ZFEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS1563-06252313-741X10.15789/1563-0625-2017-5-597-604https://doaj.org/article/f91abe7107fe4639984b0184246d413c2017-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1361https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XThe aim of the work was to study the parameters of T-cells, humoral immunity and activity of NAD(P)- dependent dehydrogenases in lymphocytes from peripheral blood at young children with recurrent obstructive bronchitis (ROB). Patients and Methods: Fifty-four children at the age of 1-3 years with ROB were observed. The control group consisted of 35 healthy children at similar age. The numbers of СD3+, СD4+, СD8+, СD19+ cells in peripheral blood were determined by flow cytofluorimetric technique. IgA, IgM, and IgG concentrations in blood serum were evaluated by G. Mancini et al. (1965); circulating immune complexes (CIC), by V. Haskova et al. (1978); total IgE test was performed with “Total IgE – IFA Best” system (Russia). Relative activity of NAD (P)-dependent dehydrogenase in peripheral blood lymphocytes was studied according to A. Savchenko et al.(1989). The results of this study have revealed changed phenotypic spectrum of peripheral blood lymphocyte populations; a statistically significant reduction in IgA concentration, and a tendency for increased IgE levels in blood serum in the children with ROB. An increase of ribose-5-phosphate- and NADPdependent metabolic processes; reduced lactate dehydrogenase anaerobic reactions; a switch from lipid catabolism products to glycolysis; a shunt role of malate/aspartate in cell energy supply, and for glutathione reductase activity; elevated levels of substrate flow along Krebs cycle in peripheral blood lymphocytes were registered as well. It may be concluded that the change of phenotype and enzymatic activity of blood lymphocytes, selective IgA deficiency in blood serum could be shown in the younger children with recurrent obstructive bronchitis.L. M. KurtasovaN. A. ShakinaSPb RAACIarticlebronchial obstructionbronchitisrecurrentlymphocytephenotypeimmunoglobulinsmetabolismImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 19, Iss 5, Pp 597-604 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	bronchial obstruction bronchitis recurrent lymphocyte phenotype immunoglobulins metabolism Immunologic diseases. Allergy RC581-607
spellingShingle	bronchial obstruction bronchitis recurrent lymphocyte phenotype immunoglobulins metabolism Immunologic diseases. Allergy RC581-607 L. M. Kurtasova N. A. Shakina FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS
description	The aim of the work was to study the parameters of T-cells, humoral immunity and activity of NAD(P)- dependent dehydrogenases in lymphocytes from peripheral blood at young children with recurrent obstructive bronchitis (ROB). Patients and Methods: Fifty-four children at the age of 1-3 years with ROB were observed. The control group consisted of 35 healthy children at similar age. The numbers of СD3+, СD4+, СD8+, СD19+ cells in peripheral blood were determined by flow cytofluorimetric technique. IgA, IgM, and IgG concentrations in blood serum were evaluated by G. Mancini et al. (1965); circulating immune complexes (CIC), by V. Haskova et al. (1978); total IgE test was performed with “Total IgE – IFA Best” system (Russia). Relative activity of NAD (P)-dependent dehydrogenase in peripheral blood lymphocytes was studied according to A. Savchenko et al.(1989). The results of this study have revealed changed phenotypic spectrum of peripheral blood lymphocyte populations; a statistically significant reduction in IgA concentration, and a tendency for increased IgE levels in blood serum in the children with ROB. An increase of ribose-5-phosphate- and NADPdependent metabolic processes; reduced lactate dehydrogenase anaerobic reactions; a switch from lipid catabolism products to glycolysis; a shunt role of malate/aspartate in cell energy supply, and for glutathione reductase activity; elevated levels of substrate flow along Krebs cycle in peripheral blood lymphocytes were registered as well. It may be concluded that the change of phenotype and enzymatic activity of blood lymphocytes, selective IgA deficiency in blood serum could be shown in the younger children with recurrent obstructive bronchitis.
format	article
author	L. M. Kurtasova N. A. Shakina
author_facet	L. M. Kurtasova N. A. Shakina
author_sort	L. M. Kurtasova
title	FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS
title_short	FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS
title_full	FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS
title_fullStr	FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS
title_full_unstemmed	FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS
title_sort	features of immune response and metabolic changes in lymphocytes from peripheral blood in younger children with recurrent obstructive bronchitis
publisher	SPb RAACI
publishDate	2017
url	https://doaj.org/article/f91abe7107fe4639984b0184246d413c
work_keys_str_mv	AT lmkurtasova featuresofimmuneresponseandmetabolicchangesinlymphocytesfromperipheralbloodinyoungerchildrenwithrecurrentobstructivebronchitis AT nashakina featuresofimmuneresponseandmetabolicchangesinlymphocytesfromperipheralbloodinyoungerchildrenwithrecurrentobstructivebronchitis
_version_	1718422392300109824

FEATURES OF IMMUNE RESPONSE AND METABOLIC CHANGES IN LYMPHOCYTES FROM PERIPHERAL BLOOD IN YOUNGER CHILDREN WITH RECURRENT OBSTRUCTIVE BRONCHITIS

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