Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken

Abstract Campylobacter jejuni is a microaerophilic foodborne pathogen that is sensitive to stress conditions. However, it is not yet understood how this stress-sensitive pathogen may cause a significant number of cases of human gastroenteritis worldwide. In this study, we examined stress tolerance i...

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Autores principales:	Euna Oh, Katelyn J. Andrews, Lynn M. McMullen, Byeonghwa Jeon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f91e574952c64295ad6783317393f373
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spelling	oai:doaj.org-article:f91e574952c64295ad6783317393f3732021-12-02T16:08:27ZTolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken10.1038/s41598-019-48373-02045-2322https://doaj.org/article/f91e574952c64295ad6783317393f3732019-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-48373-0https://doaj.org/toc/2045-2322Abstract Campylobacter jejuni is a microaerophilic foodborne pathogen that is sensitive to stress conditions. However, it is not yet understood how this stress-sensitive pathogen may cause a significant number of cases of human gastroenteritis worldwide. In this study, we examined stress tolerance in 70 C. jejuni strains isolated from retail chicken under several stress conditions related to food safety. Compared to oxygen-sensitive (OS) strains of C. jejuni, C. jejuni strains with increased aerotolerance, such as hyper-aerotolerant (HAT) and aerotolerant (AT) strains, were more tolerant to peracetic acid, refrigeration and freeze-thaw stresses. However, the levels of thermotolerance and hyper-osmotolerance were not associated with the aerotolerance level of C. jejuni. The HAT and AT strains of C. jejuni exhibited significantly increased activities of catalase and superoxide dismutase (SOD), compared to the OS strains. Consistently, the HAT and AT strains were highly tolerant to oxidants, such as hydrogen peroxide, cumene hydroperoxide and menadione, compared to the OS strains. The AT and HAT strains that were tolerant to stresses, particularly peracetic acid and refrigeration, predominantly belonged to multilocus sequence typing (MLST) clonal complex (CC)-21. This study shows that oxidative stress resistance plays a role in determining the differential level of aerotolerance in C. jejuni and that AT and HAT strains of C. jejuni are more tolerant to oxidants and low temperatures than OS strains.Euna OhKatelyn J. AndrewsLynn M. McMullenByeonghwa JeonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-9 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Euna Oh Katelyn J. Andrews Lynn M. McMullen Byeonghwa Jeon Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken
description	Abstract Campylobacter jejuni is a microaerophilic foodborne pathogen that is sensitive to stress conditions. However, it is not yet understood how this stress-sensitive pathogen may cause a significant number of cases of human gastroenteritis worldwide. In this study, we examined stress tolerance in 70 C. jejuni strains isolated from retail chicken under several stress conditions related to food safety. Compared to oxygen-sensitive (OS) strains of C. jejuni, C. jejuni strains with increased aerotolerance, such as hyper-aerotolerant (HAT) and aerotolerant (AT) strains, were more tolerant to peracetic acid, refrigeration and freeze-thaw stresses. However, the levels of thermotolerance and hyper-osmotolerance were not associated with the aerotolerance level of C. jejuni. The HAT and AT strains of C. jejuni exhibited significantly increased activities of catalase and superoxide dismutase (SOD), compared to the OS strains. Consistently, the HAT and AT strains were highly tolerant to oxidants, such as hydrogen peroxide, cumene hydroperoxide and menadione, compared to the OS strains. The AT and HAT strains that were tolerant to stresses, particularly peracetic acid and refrigeration, predominantly belonged to multilocus sequence typing (MLST) clonal complex (CC)-21. This study shows that oxidative stress resistance plays a role in determining the differential level of aerotolerance in C. jejuni and that AT and HAT strains of C. jejuni are more tolerant to oxidants and low temperatures than OS strains.
format	article
author	Euna Oh Katelyn J. Andrews Lynn M. McMullen Byeonghwa Jeon
author_facet	Euna Oh Katelyn J. Andrews Lynn M. McMullen Byeonghwa Jeon
author_sort	Euna Oh
title	Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken
title_short	Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken
title_full	Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken
title_fullStr	Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken
title_full_unstemmed	Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken
title_sort	tolerance to stress conditions associated with food safety in campylobacter jejuni strains isolated from retail raw chicken
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/f91e574952c64295ad6783317393f373
work_keys_str_mv	AT eunaoh tolerancetostressconditionsassociatedwithfoodsafetyincampylobacterjejunistrainsisolatedfromretailrawchicken AT katelynjandrews tolerancetostressconditionsassociatedwithfoodsafetyincampylobacterjejunistrainsisolatedfromretailrawchicken AT lynnmmcmullen tolerancetostressconditionsassociatedwithfoodsafetyincampylobacterjejunistrainsisolatedfromretailrawchicken AT byeonghwajeon tolerancetostressconditionsassociatedwithfoodsafetyincampylobacterjejunistrainsisolatedfromretailrawchicken
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Tolerance to stress conditions associated with food safety in Campylobacter jejuni strains isolated from retail raw chicken

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