Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222

Abstract Denitrification is a respiratory process by which nitrate is reduced to dinitrogen. Incomplete denitrification results in the emission of the greenhouse gas nitrous oxide and this is potentiated in acidic soils, which display reduced denitrification rates and high N2O/N2 ratios compared to...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Alfonso Olaya-Abril, Jesús Hidalgo-Carrillo, Víctor M. Luque-Almagro, Carlos Fuentes-Almagro, Francisco J. Urbano, Conrado Moreno-Vivián, David J. Richardson, María Dolores Roldán
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f926e2aa80984d3084a42aea3a554398
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f926e2aa80984d3084a42aea3a554398
record_format	dspace
spelling	oai:doaj.org-article:f926e2aa80984d3084a42aea3a5543982021-12-02T16:34:53ZEffect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD122210.1038/s41598-021-96559-22045-2322https://doaj.org/article/f926e2aa80984d3084a42aea3a5543982021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96559-2https://doaj.org/toc/2045-2322Abstract Denitrification is a respiratory process by which nitrate is reduced to dinitrogen. Incomplete denitrification results in the emission of the greenhouse gas nitrous oxide and this is potentiated in acidic soils, which display reduced denitrification rates and high N2O/N2 ratios compared to alkaline soils. In this work, impact of pH on the proteome of the soil denitrifying bacterium Paracoccus denitrificans PD1222 was analysed with nitrate as sole energy and nitrogen source under anaerobic conditions at pH ranging from 6.5 to 7.5. Quantitative proteomic analysis revealed that the highest difference in protein representation was observed when the proteome at pH 6.5 was compared to the reference proteome at pH 7.2. However, this difference in the extracellular pH was not enough to produce modification of intracellular pH, which was maintained at 6.5 ± 0.1. The biosynthetic pathways of several cofactors relevant for denitrification and nitrogen assimilation like cobalamin, riboflavin, molybdopterin and nicotinamide were negatively affected at pH 6.5. In addition, peptide representation of reductases involved in nitrate assimilation and denitrification were reduced at pH 6.5. Data highlight the strong negative impact of pH on NosZ synthesis and intracellular copper content, thus impairing active NosZ assembly and, in turn, leading to elevated nitrous oxide emissions.Alfonso Olaya-AbrilJesús Hidalgo-CarrilloVíctor M. Luque-AlmagroCarlos Fuentes-AlmagroFrancisco J. UrbanoConrado Moreno-ViviánDavid J. RichardsonMaría Dolores RoldánNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alfonso Olaya-Abril Jesús Hidalgo-Carrillo Víctor M. Luque-Almagro Carlos Fuentes-Almagro Francisco J. Urbano Conrado Moreno-Vivián David J. Richardson María Dolores Roldán Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222
description	Abstract Denitrification is a respiratory process by which nitrate is reduced to dinitrogen. Incomplete denitrification results in the emission of the greenhouse gas nitrous oxide and this is potentiated in acidic soils, which display reduced denitrification rates and high N2O/N2 ratios compared to alkaline soils. In this work, impact of pH on the proteome of the soil denitrifying bacterium Paracoccus denitrificans PD1222 was analysed with nitrate as sole energy and nitrogen source under anaerobic conditions at pH ranging from 6.5 to 7.5. Quantitative proteomic analysis revealed that the highest difference in protein representation was observed when the proteome at pH 6.5 was compared to the reference proteome at pH 7.2. However, this difference in the extracellular pH was not enough to produce modification of intracellular pH, which was maintained at 6.5 ± 0.1. The biosynthetic pathways of several cofactors relevant for denitrification and nitrogen assimilation like cobalamin, riboflavin, molybdopterin and nicotinamide were negatively affected at pH 6.5. In addition, peptide representation of reductases involved in nitrate assimilation and denitrification were reduced at pH 6.5. Data highlight the strong negative impact of pH on NosZ synthesis and intracellular copper content, thus impairing active NosZ assembly and, in turn, leading to elevated nitrous oxide emissions.
format	article
author	Alfonso Olaya-Abril Jesús Hidalgo-Carrillo Víctor M. Luque-Almagro Carlos Fuentes-Almagro Francisco J. Urbano Conrado Moreno-Vivián David J. Richardson María Dolores Roldán
author_facet	Alfonso Olaya-Abril Jesús Hidalgo-Carrillo Víctor M. Luque-Almagro Carlos Fuentes-Almagro Francisco J. Urbano Conrado Moreno-Vivián David J. Richardson María Dolores Roldán
author_sort	Alfonso Olaya-Abril
title	Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222
title_short	Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222
title_full	Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222
title_fullStr	Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222
title_full_unstemmed	Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222
title_sort	effect of ph on the denitrification proteome of the soil bacterium paracoccus denitrificans pd1222
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f926e2aa80984d3084a42aea3a554398
work_keys_str_mv	AT alfonsoolayaabril effectofphonthedenitrificationproteomeofthesoilbacteriumparacoccusdenitrificanspd1222 AT jesushidalgocarrillo effectofphonthedenitrificationproteomeofthesoilbacteriumparacoccusdenitrificanspd1222 AT victormluquealmagro effectofphonthedenitrificationproteomeofthesoilbacteriumparacoccusdenitrificanspd1222 AT carlosfuentesalmagro effectofphonthedenitrificationproteomeofthesoilbacteriumparacoccusdenitrificanspd1222 AT franciscojurbano effectofphonthedenitrificationproteomeofthesoilbacteriumparacoccusdenitrificanspd1222 AT conradomorenovivian effectofphonthedenitrificationproteomeofthesoilbacteriumparacoccusdenitrificanspd1222 AT davidjrichardson effectofphonthedenitrificationproteomeofthesoilbacteriumparacoccusdenitrificanspd1222 AT mariadoloresroldan effectofphonthedenitrificationproteomeofthesoilbacteriumparacoccusdenitrificanspd1222
_version_	1718383790047363072

Effect of pH on the denitrification proteome of the soil bacterium Paracoccus denitrificans PD1222

Ejemplares similares