Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens

Abstract Invasion of periodontal tissues by Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can be associated with aggressive forms of periodontitis. Oleoresins from different copaifera species and their compounds display various pharmacological properties. The present study evalu...

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Autores principales:	Fariza Abrão, Thayná Souza Silva, Claudia L. Moura, Sérgio Ricardo Ambrósio, Rodrigo Cassio Sola Veneziani, Raphael E. F. de Paiva, Jairo Kenupp Bastos, Carlos Henrique Gomes Martins
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f9293a7698cc42f78fa6ee26d5884e08
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spelling	oai:doaj.org-article:f9293a7698cc42f78fa6ee26d5884e082021-12-02T13:34:57ZOleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens10.1038/s41598-021-84480-72045-2322https://doaj.org/article/f9293a7698cc42f78fa6ee26d5884e082021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84480-7https://doaj.org/toc/2045-2322Abstract Invasion of periodontal tissues by Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can be associated with aggressive forms of periodontitis. Oleoresins from different copaifera species and their compounds display various pharmacological properties. The present study evaluates the antibacterial and antivirulence activity of oleoresins obtained from different copaifera species and of ten isolated compounds against two causative agents of periodontitis. The following assays were performed: determination of the minimum inhibitory concentration (MIC), determination of the minimum bactericidal concentration (MBC), and determination of the antibiofilm activity by inhibition of biofilm formation and biofilm eradication tests. The antivirulence activity was assessed by hemagglutination, P. gingivalis Arg-X and Lis-X cysteine protease inhibition assay, and A. actinomycetemcomitans leukotoxin inhibition assay. The MIC and MBC of the oleoresins and isolated compounds 1, 2, and 3 ranged from 1.59 to 50 μg/mL against P. gingivalis (ATCC 33277) and clinical isolates and from 6.25 to 400 μg/mL against A. actinomycetemcomitans (ATCC 43717) and clinical isolates. About the antibiofilm activity, the oleoresins and isolated compounds 1, 2, and 3 inhibited biofilm formation by at least 50% and eradicated pre-formed P. gingivalis and A. actinomycetemcomitans biofilms in the monospecies and multispecies modes. A promising activity concerning cysteine protease and leucotoxin inhibition was also evident. In addition, molecular docking analysis was performed. The investigated oleoresins and their compounds may play an important role in the search for novel sources of agents that can act against periodontal pathogens.Fariza AbrãoThayná Souza SilvaClaudia L. MouraSérgio Ricardo AmbrósioRodrigo Cassio Sola VenezianiRaphael E. F. de PaivaJairo Kenupp BastosCarlos Henrique Gomes MartinsNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fariza Abrão Thayná Souza Silva Claudia L. Moura Sérgio Ricardo Ambrósio Rodrigo Cassio Sola Veneziani Raphael E. F. de Paiva Jairo Kenupp Bastos Carlos Henrique Gomes Martins Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens
description	Abstract Invasion of periodontal tissues by Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans can be associated with aggressive forms of periodontitis. Oleoresins from different copaifera species and their compounds display various pharmacological properties. The present study evaluates the antibacterial and antivirulence activity of oleoresins obtained from different copaifera species and of ten isolated compounds against two causative agents of periodontitis. The following assays were performed: determination of the minimum inhibitory concentration (MIC), determination of the minimum bactericidal concentration (MBC), and determination of the antibiofilm activity by inhibition of biofilm formation and biofilm eradication tests. The antivirulence activity was assessed by hemagglutination, P. gingivalis Arg-X and Lis-X cysteine protease inhibition assay, and A. actinomycetemcomitans leukotoxin inhibition assay. The MIC and MBC of the oleoresins and isolated compounds 1, 2, and 3 ranged from 1.59 to 50 μg/mL against P. gingivalis (ATCC 33277) and clinical isolates and from 6.25 to 400 μg/mL against A. actinomycetemcomitans (ATCC 43717) and clinical isolates. About the antibiofilm activity, the oleoresins and isolated compounds 1, 2, and 3 inhibited biofilm formation by at least 50% and eradicated pre-formed P. gingivalis and A. actinomycetemcomitans biofilms in the monospecies and multispecies modes. A promising activity concerning cysteine protease and leucotoxin inhibition was also evident. In addition, molecular docking analysis was performed. The investigated oleoresins and their compounds may play an important role in the search for novel sources of agents that can act against periodontal pathogens.
format	article
author	Fariza Abrão Thayná Souza Silva Claudia L. Moura Sérgio Ricardo Ambrósio Rodrigo Cassio Sola Veneziani Raphael E. F. de Paiva Jairo Kenupp Bastos Carlos Henrique Gomes Martins
author_facet	Fariza Abrão Thayná Souza Silva Claudia L. Moura Sérgio Ricardo Ambrósio Rodrigo Cassio Sola Veneziani Raphael E. F. de Paiva Jairo Kenupp Bastos Carlos Henrique Gomes Martins
author_sort	Fariza Abrão
title	Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens
title_short	Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens
title_full	Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens
title_fullStr	Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens
title_full_unstemmed	Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens
title_sort	oleoresins and naturally occurring compounds of copaifera genus as antibacterial and antivirulence agents against periodontal pathogens
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f9293a7698cc42f78fa6ee26d5884e08
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Oleoresins and naturally occurring compounds of Copaifera genus as antibacterial and antivirulence agents against periodontal pathogens

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