Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel

Abstract Protein-metal ion interactions are ubiquitous in nature and can be utilized for controlling the self-assembly of complex supramolecular architectures and materials. Here, a tunable supramolecular hydrogel is described, obtained by self-assembly of a Zn2+-responsive peptide-hyaluronic acid h...

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Autores principales:	Robert Selegård, Christopher Aronsson, Caroline Brommesson, Staffan Dånmark, Daniel Aili
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f929e2157b3b4000bef943ea1bf2448e
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spelling	oai:doaj.org-article:f929e2157b3b4000bef943ea1bf2448e2021-12-02T16:06:28ZFolding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel10.1038/s41598-017-06457-92045-2322https://doaj.org/article/f929e2157b3b4000bef943ea1bf2448e2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06457-9https://doaj.org/toc/2045-2322Abstract Protein-metal ion interactions are ubiquitous in nature and can be utilized for controlling the self-assembly of complex supramolecular architectures and materials. Here, a tunable supramolecular hydrogel is described, obtained by self-assembly of a Zn2+-responsive peptide-hyaluronic acid hybrid synthesized using strain promoted click chemistry. Addition of Zn2+ triggers folding of the peptides into a helix-loop-helix motif and dimerization into four-helix bundles, resulting in hydrogelation. Removal of the Zn2+ by chelators results in rapid hydrogel disassembly. Degradation of the hydrogels can also be time-programed by encapsulation of a hydrolyzing enzyme within the gel, offering multiple possibilities for modulating materials properties and release of encapsulated species. The hydrogel further shows potential antioxidant properties when evaluated using an in vitro model for reactive oxygen species.Robert SelegårdChristopher AronssonCaroline BrommessonStaffan DånmarkDaniel AiliNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Robert Selegård Christopher Aronsson Caroline Brommesson Staffan Dånmark Daniel Aili Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel
description	Abstract Protein-metal ion interactions are ubiquitous in nature and can be utilized for controlling the self-assembly of complex supramolecular architectures and materials. Here, a tunable supramolecular hydrogel is described, obtained by self-assembly of a Zn2+-responsive peptide-hyaluronic acid hybrid synthesized using strain promoted click chemistry. Addition of Zn2+ triggers folding of the peptides into a helix-loop-helix motif and dimerization into four-helix bundles, resulting in hydrogelation. Removal of the Zn2+ by chelators results in rapid hydrogel disassembly. Degradation of the hydrogels can also be time-programed by encapsulation of a hydrolyzing enzyme within the gel, offering multiple possibilities for modulating materials properties and release of encapsulated species. The hydrogel further shows potential antioxidant properties when evaluated using an in vitro model for reactive oxygen species.
format	article
author	Robert Selegård Christopher Aronsson Caroline Brommesson Staffan Dånmark Daniel Aili
author_facet	Robert Selegård Christopher Aronsson Caroline Brommesson Staffan Dånmark Daniel Aili
author_sort	Robert Selegård
title	Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel
title_short	Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel
title_full	Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel
title_fullStr	Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel
title_full_unstemmed	Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel
title_sort	folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f929e2157b3b4000bef943ea1bf2448e
work_keys_str_mv	AT robertselegard foldingdrivenselfassemblyofastimuliresponsivepeptidehyaluronanhybridhydrogel AT christopheraronsson foldingdrivenselfassemblyofastimuliresponsivepeptidehyaluronanhybridhydrogel AT carolinebrommesson foldingdrivenselfassemblyofastimuliresponsivepeptidehyaluronanhybridhydrogel AT staffandanmark foldingdrivenselfassemblyofastimuliresponsivepeptidehyaluronanhybridhydrogel AT danielaili foldingdrivenselfassemblyofastimuliresponsivepeptidehyaluronanhybridhydrogel
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Folding driven self-assembly of a stimuli-responsive peptide-hyaluronan hybrid hydrogel

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