Uptake of bright fluorophore core-silica shell nanoparticles by biological systems

Andrew Zane,1 Christie McCracken,2 Deborah A Knight,2 Tanya Young,1 Anthony D Lutton,3 John W Olesik,3 W James Waldman,2 Prabir K Dutta1 1Department of Chemistry and Biochemistry, 2Department of Pathology, 3School of Earth Sciences, The Ohio State University, Columbus, OH, USA Abstract: Nanoparti...

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Autores principales:	Zane A, McCracken C, Knight DA, Young T, Lutton AD, Olesik JW, Waldman WJ, Dutta PK
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f92a8905122a455494d7a9e6ff50e336
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spelling	oai:doaj.org-article:f92a8905122a455494d7a9e6ff50e3362021-12-02T00:43:21ZUptake of bright fluorophore core-silica shell nanoparticles by biological systems1178-2013https://doaj.org/article/f92a8905122a455494d7a9e6ff50e3362015-02-01T00:00:00Zhttp://www.dovepress.com/uptake-of-bright-fluorophore-core-silica-shell-nanoparticles-by-biolog-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Andrew Zane,1 Christie McCracken,2 Deborah A Knight,2 Tanya Young,1 Anthony D Lutton,3 John W Olesik,3 W James Waldman,2 Prabir K Dutta1 1Department of Chemistry and Biochemistry, 2Department of Pathology, 3School of Earth Sciences, The Ohio State University, Columbus, OH, USA Abstract: Nanoparticles are used in a variety of consumer applications. Silica nanoparticles in particular are common, including as a component of foods. There are concerns that ingested nanosilica particles can cross the intestinal epithelium, enter the circulation, and accumulate in tissues and organs. Thus, tracking these particles is of interest, and fluorescence spectroscopic methods are well-suited for this purpose. However, nanosilica is not fluorescent. In this article, we focus on core-silica shell nanoparticles, using fluorescent Rhodamine 6G, Rhodamine 800, or CdSe/CdS/ZnS quantum dots as the core. These stable fluorophore/silica nanoparticles had surface characteristics similar to those of commercial silica particles. Thus, they were used as model particles to examine internalization by cultured cells, including an epithelial cell line relevant to the gastrointestinal tract. Finally, these particles were administered to mice by gavage, and their presence in various organs, including stomach, small intestine, cecum, colon, kidney, lung, brain, and spleen, was examined. By combining confocal fluorescence microscopy with inductively coupled plasma mass spectrometry, the presence of nanoparticles, rather than their dissolved form, was established in liver tissues. Keywords: quantum dots, dyes, optical spectroscopy, NMR, zeta potential, mouse model, macrophages, Caco-2, nanoparticles in foodsZane AMcCracken CKnight DAYoung TLutton ADOlesik JWWaldman WJDutta PKDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 1547-1567 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Zane A McCracken C Knight DA Young T Lutton AD Olesik JW Waldman WJ Dutta PK Uptake of bright fluorophore core-silica shell nanoparticles by biological systems
description	Andrew Zane,1 Christie McCracken,2 Deborah A Knight,2 Tanya Young,1 Anthony D Lutton,3 John W Olesik,3 W James Waldman,2 Prabir K Dutta1 1Department of Chemistry and Biochemistry, 2Department of Pathology, 3School of Earth Sciences, The Ohio State University, Columbus, OH, USA Abstract: Nanoparticles are used in a variety of consumer applications. Silica nanoparticles in particular are common, including as a component of foods. There are concerns that ingested nanosilica particles can cross the intestinal epithelium, enter the circulation, and accumulate in tissues and organs. Thus, tracking these particles is of interest, and fluorescence spectroscopic methods are well-suited for this purpose. However, nanosilica is not fluorescent. In this article, we focus on core-silica shell nanoparticles, using fluorescent Rhodamine 6G, Rhodamine 800, or CdSe/CdS/ZnS quantum dots as the core. These stable fluorophore/silica nanoparticles had surface characteristics similar to those of commercial silica particles. Thus, they were used as model particles to examine internalization by cultured cells, including an epithelial cell line relevant to the gastrointestinal tract. Finally, these particles were administered to mice by gavage, and their presence in various organs, including stomach, small intestine, cecum, colon, kidney, lung, brain, and spleen, was examined. By combining confocal fluorescence microscopy with inductively coupled plasma mass spectrometry, the presence of nanoparticles, rather than their dissolved form, was established in liver tissues. Keywords: quantum dots, dyes, optical spectroscopy, NMR, zeta potential, mouse model, macrophages, Caco-2, nanoparticles in foods
format	article
author	Zane A McCracken C Knight DA Young T Lutton AD Olesik JW Waldman WJ Dutta PK
author_facet	Zane A McCracken C Knight DA Young T Lutton AD Olesik JW Waldman WJ Dutta PK
author_sort	Zane A
title	Uptake of bright fluorophore core-silica shell nanoparticles by biological systems
title_short	Uptake of bright fluorophore core-silica shell nanoparticles by biological systems
title_full	Uptake of bright fluorophore core-silica shell nanoparticles by biological systems
title_fullStr	Uptake of bright fluorophore core-silica shell nanoparticles by biological systems
title_full_unstemmed	Uptake of bright fluorophore core-silica shell nanoparticles by biological systems
title_sort	uptake of bright fluorophore core-silica shell nanoparticles by biological systems
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/f92a8905122a455494d7a9e6ff50e336
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Uptake of bright fluorophore core-silica shell nanoparticles by biological systems

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