Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules

Proteins evolve through the modular rearrangement of domains. Here the authors introduce MISER, a minimization by iterative size-exclusion and recombination method to make all possible deletions of a protein, uncovering functions for Cas9 domains involved in DNA binding.

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Autores principales:	Arik Shams, Sean A. Higgins, Christof Fellmann, Thomas G. Laughlin, Benjamin L. Oakes, Rachel Lew, Shin Kim, Maria Lukarska, Madeline Arnold, Brett T. Staahl, Jennifer A. Doudna, David F. Savage
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f92b1a4bf0574fe9b95344d8bcec8e40
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spelling	oai:doaj.org-article:f92b1a4bf0574fe9b95344d8bcec8e402021-12-02T17:37:42ZComprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules10.1038/s41467-021-25992-82041-1723https://doaj.org/article/f92b1a4bf0574fe9b95344d8bcec8e402021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25992-8https://doaj.org/toc/2041-1723Proteins evolve through the modular rearrangement of domains. Here the authors introduce MISER, a minimization by iterative size-exclusion and recombination method to make all possible deletions of a protein, uncovering functions for Cas9 domains involved in DNA binding.Arik ShamsSean A. HigginsChristof FellmannThomas G. LaughlinBenjamin L. OakesRachel LewShin KimMaria LukarskaMadeline ArnoldBrett T. StaahlJennifer A. DoudnaDavid F. SavageNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Arik Shams Sean A. Higgins Christof Fellmann Thomas G. Laughlin Benjamin L. Oakes Rachel Lew Shin Kim Maria Lukarska Madeline Arnold Brett T. Staahl Jennifer A. Doudna David F. Savage Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
description	Proteins evolve through the modular rearrangement of domains. Here the authors introduce MISER, a minimization by iterative size-exclusion and recombination method to make all possible deletions of a protein, uncovering functions for Cas9 domains involved in DNA binding.
format	article
author	Arik Shams Sean A. Higgins Christof Fellmann Thomas G. Laughlin Benjamin L. Oakes Rachel Lew Shin Kim Maria Lukarska Madeline Arnold Brett T. Staahl Jennifer A. Doudna David F. Savage
author_facet	Arik Shams Sean A. Higgins Christof Fellmann Thomas G. Laughlin Benjamin L. Oakes Rachel Lew Shin Kim Maria Lukarska Madeline Arnold Brett T. Staahl Jennifer A. Doudna David F. Savage
author_sort	Arik Shams
title	Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
title_short	Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
title_full	Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
title_fullStr	Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
title_full_unstemmed	Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules
title_sort	comprehensive deletion landscape of crispr-cas9 identifies minimal rna-guided dna-binding modules
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/f92b1a4bf0574fe9b95344d8bcec8e40
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Comprehensive deletion landscape of CRISPR-Cas9 identifies minimal RNA-guided DNA-binding modules

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