Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.

Here, we proposed that volatile organic compounds (VOC), specifically methyl salicylate (MeSA), mediate the formation of calcium oxalate crystals (COC) in the defence against ozone (O3) oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O3...

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Autores principales:	Poliana Cardoso-Gustavson, Vanessa Palermo Bolsoni, Debora Pinheiro de Oliveira, Maria Tereza Gromboni Guaratini, Marcos Pereira Marinho Aidar, Mauro Alexandre Marabesi, Edenise Segala Alves, Silvia Ribeiro de Souza
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f92d8ed67a66443881b18eea028834c6
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spelling	oai:doaj.org-article:f92d8ed67a66443881b18eea028834c62021-11-25T06:02:52ZOzone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.1932-620310.1371/journal.pone.0105072https://doaj.org/article/f92d8ed67a66443881b18eea028834c62014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25165889/?tool=EBIhttps://doaj.org/toc/1932-6203Here, we proposed that volatile organic compounds (VOC), specifically methyl salicylate (MeSA), mediate the formation of calcium oxalate crystals (COC) in the defence against ozone (O3) oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O3 and widely distributed in the Brazilian forest. This species constitutively produces COC. We exposed plants to a controlled fumigation experiment and assessed biochemical, physiological, and morphological parameters. O3 induced a significant increase in the concentrations of constitutive oxygenated compounds, MeSA and terpenoids as well as in COC number. Our analysis supported the hypothesis that ozone-induced VOC (mainly MeSA) regulate ROS formation in a way that promotes the opening of calcium channels and the subsequent formation of COC in a fast and stable manner to stop the consequences of the reactive oxygen species in the tissue, indeed immobilising the excess calcium (caused by acute exposition to O3) that can be dangerous to the plant. To test this hypothesis, we performed an independent experiment spraying MeSA over C. floribundus plants and observed an increase in the number of COC, indicating that this compound has a potential to directly induce their formation. Thus, the tolerance of C. floribundus to O3 oxidative stress could be a consequence of a higher capacity for the production of VOC and COC rather than the modulation of antioxidant balance. We also present some insights into constitutive morphological features that may be related to the tolerance that this species exhibits to O3.Poliana Cardoso-GustavsonVanessa Palermo BolsoniDebora Pinheiro de OliveiraMaria Tereza Gromboni GuaratiniMarcos Pereira Marinho AidarMauro Alexandre MarabesiEdenise Segala AlvesSilvia Ribeiro de SouzaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 8, p e105072 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Poliana Cardoso-Gustavson Vanessa Palermo Bolsoni Debora Pinheiro de Oliveira Maria Tereza Gromboni Guaratini Marcos Pereira Marinho Aidar Mauro Alexandre Marabesi Edenise Segala Alves Silvia Ribeiro de Souza Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.
description	Here, we proposed that volatile organic compounds (VOC), specifically methyl salicylate (MeSA), mediate the formation of calcium oxalate crystals (COC) in the defence against ozone (O3) oxidative damage. We performed experiments using Croton floribundus, a pioneer tree species that is tolerant to O3 and widely distributed in the Brazilian forest. This species constitutively produces COC. We exposed plants to a controlled fumigation experiment and assessed biochemical, physiological, and morphological parameters. O3 induced a significant increase in the concentrations of constitutive oxygenated compounds, MeSA and terpenoids as well as in COC number. Our analysis supported the hypothesis that ozone-induced VOC (mainly MeSA) regulate ROS formation in a way that promotes the opening of calcium channels and the subsequent formation of COC in a fast and stable manner to stop the consequences of the reactive oxygen species in the tissue, indeed immobilising the excess calcium (caused by acute exposition to O3) that can be dangerous to the plant. To test this hypothesis, we performed an independent experiment spraying MeSA over C. floribundus plants and observed an increase in the number of COC, indicating that this compound has a potential to directly induce their formation. Thus, the tolerance of C. floribundus to O3 oxidative stress could be a consequence of a higher capacity for the production of VOC and COC rather than the modulation of antioxidant balance. We also present some insights into constitutive morphological features that may be related to the tolerance that this species exhibits to O3.
format	article
author	Poliana Cardoso-Gustavson Vanessa Palermo Bolsoni Debora Pinheiro de Oliveira Maria Tereza Gromboni Guaratini Marcos Pereira Marinho Aidar Mauro Alexandre Marabesi Edenise Segala Alves Silvia Ribeiro de Souza
author_facet	Poliana Cardoso-Gustavson Vanessa Palermo Bolsoni Debora Pinheiro de Oliveira Maria Tereza Gromboni Guaratini Marcos Pereira Marinho Aidar Mauro Alexandre Marabesi Edenise Segala Alves Silvia Ribeiro de Souza
author_sort	Poliana Cardoso-Gustavson
title	Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.
title_short	Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.
title_full	Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.
title_fullStr	Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.
title_full_unstemmed	Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.
title_sort	ozone-induced responses in croton floribundus spreng. (euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/f92d8ed67a66443881b18eea028834c6
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Ozone-induced responses in Croton floribundus Spreng. (Euphorbiaceae): metabolic cross-talk between volatile organic compounds and calcium oxalate crystal formation.

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