Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds

BACKGROUND AND OBJECTIVE: Pseudomonas aeruginosa is one of the main causes of hospital infections. Pathogenic factors in this bacterium may play a role in the resistance to carbapenem and beta-lactam. The purpose of this study was to evaluate the role and function of KPC and MBL enzymes in increasin...

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Autores principales:	H Tahmasebi, F Maleki, S Dehbashi, MR Arabestani
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2019
Materias:	antibiotic resistance pseudomonas aeruginosa virulence factors carbapenem antibiotics. Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/f92e4ab9e4bd484e866f7d0f58f55e8d
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spelling	oai:doaj.org-article:f92e4ab9e4bd484e866f7d0f58f55e8d2021-11-10T08:30:36ZRole and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds1561-41072251-7170https://doaj.org/article/f92e4ab9e4bd484e866f7d0f58f55e8d2019-12-01T00:00:00Zhttp://jbums.org/article-1-8049-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Pseudomonas aeruginosa is one of the main causes of hospital infections. Pathogenic factors in this bacterium may play a role in the resistance to carbapenem and beta-lactam. The purpose of this study was to evaluate the role and function of KPC and MBL enzymes in increasing the pathogenicity of Pseudomonas aeruginosa isolated from burn wounds. METHODS: In this cross-sectional study, 63 isolates of Pseudomonas aeruginosa from burn wounds of different patients were isolated using biochemical tests such as fermentation of sugars in the OF medium, oxidase test, and so on. Determination of resistance pattern and strains with metallobetalactamase and carbapenema was done by disc diffusion method. The oprD gene was used for molecular confirmation of isolates. PCR method was used to detect pathogenicity genes. FINDINGS: Out of 63 isolates of Pseudomonas aeruginosa isolated from burn patients, 10 isolates (15.83%) had KPC enzyme and 13 isolates (20.63%) had MBL enzymes. Doripenem, Ertapenem and meropenem were the most frequent. Also, the lasB gene was observed in 43 isolates (68.25%), plcN gene in 41 isolates (65.07%), lasA gene in 20 isolates (31.74%), apr in 60 isolates (95.23%), phzI gene in 53 isolates (84.12%), the phzII gene in 38 isolates (60.31%), phzH gene in 30 isolates (47.61%) and plcH gene in 56 isolates (88.88%). CONCLUSION: The results of this study showed that the production of Carbapnemase and MBL enzymes increased the pathogenicity of Pseudomonas aeruginosa isolated from burn wounds.H TahmasebiF MalekiS DehbashiMR ArabestaniBabol University of Medical Sciencesarticleantibiotic resistancepseudomonas aeruginosavirulence factorscarbapenem antibiotics.MedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 21, Iss 1, Pp 127-134 (2019)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	antibiotic resistance pseudomonas aeruginosa virulence factors carbapenem antibiotics. Medicine R Medicine (General) R5-920
spellingShingle	antibiotic resistance pseudomonas aeruginosa virulence factors carbapenem antibiotics. Medicine R Medicine (General) R5-920 H Tahmasebi F Maleki S Dehbashi MR Arabestani Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds
description	BACKGROUND AND OBJECTIVE: Pseudomonas aeruginosa is one of the main causes of hospital infections. Pathogenic factors in this bacterium may play a role in the resistance to carbapenem and beta-lactam. The purpose of this study was to evaluate the role and function of KPC and MBL enzymes in increasing the pathogenicity of Pseudomonas aeruginosa isolated from burn wounds. METHODS: In this cross-sectional study, 63 isolates of Pseudomonas aeruginosa from burn wounds of different patients were isolated using biochemical tests such as fermentation of sugars in the OF medium, oxidase test, and so on. Determination of resistance pattern and strains with metallobetalactamase and carbapenema was done by disc diffusion method. The oprD gene was used for molecular confirmation of isolates. PCR method was used to detect pathogenicity genes. FINDINGS: Out of 63 isolates of Pseudomonas aeruginosa isolated from burn patients, 10 isolates (15.83%) had KPC enzyme and 13 isolates (20.63%) had MBL enzymes. Doripenem, Ertapenem and meropenem were the most frequent. Also, the lasB gene was observed in 43 isolates (68.25%), plcN gene in 41 isolates (65.07%), lasA gene in 20 isolates (31.74%), apr in 60 isolates (95.23%), phzI gene in 53 isolates (84.12%), the phzII gene in 38 isolates (60.31%), phzH gene in 30 isolates (47.61%) and plcH gene in 56 isolates (88.88%). CONCLUSION: The results of this study showed that the production of Carbapnemase and MBL enzymes increased the pathogenicity of Pseudomonas aeruginosa isolated from burn wounds.
format	article
author	H Tahmasebi F Maleki S Dehbashi MR Arabestani
author_facet	H Tahmasebi F Maleki S Dehbashi MR Arabestani
author_sort	H Tahmasebi
title	Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds
title_short	Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds
title_full	Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds
title_fullStr	Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds
title_full_unstemmed	Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds
title_sort	role and function of kpc and mbl enzymes in increasing the pathogenicity of pseudomonas aeruginosa isolated from burn wounds
publisher	Babol University of Medical Sciences
publishDate	2019
url	https://doaj.org/article/f92e4ab9e4bd484e866f7d0f58f55e8d
work_keys_str_mv	AT htahmasebi roleandfunctionofkpcandmblenzymesinincreasingthepathogenicityofpseudomonasaeruginosaisolatedfromburnwounds AT fmaleki roleandfunctionofkpcandmblenzymesinincreasingthepathogenicityofpseudomonasaeruginosaisolatedfromburnwounds AT sdehbashi roleandfunctionofkpcandmblenzymesinincreasingthepathogenicityofpseudomonasaeruginosaisolatedfromburnwounds AT mrarabestani roleandfunctionofkpcandmblenzymesinincreasingthepathogenicityofpseudomonasaeruginosaisolatedfromburnwounds
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Role and Function of KPC and MBL Enzymes in Increasing the Pathogenicity of Pseudomonas Aeruginosa Isolated from Burn Wounds

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