In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain

Abstract Hundreds of inbred mouse strains are established for use in a broad spectrum of basic research fields, including genetics, neuroscience, immunology, and cancer. Inbred mice exhibit identical intra-strain genetics and divergent inter-strain phenotypes. The cognitive and behavioral divergence...

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Autores principales:	Keigo Hikishima, Yuji Komaki, Fumiko Seki, Yasuyuki Ohnishi, Hirotaka J. Okano, Hideyuki Okano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f932a284d4ac4b6bb78f5603aa7892aa
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spelling	oai:doaj.org-article:f932a284d4ac4b6bb78f5603aa7892aa2021-12-02T16:06:42ZIn vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain10.1038/s41598-017-00148-12045-2322https://doaj.org/article/f932a284d4ac4b6bb78f5603aa7892aa2017-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00148-1https://doaj.org/toc/2045-2322Abstract Hundreds of inbred mouse strains are established for use in a broad spectrum of basic research fields, including genetics, neuroscience, immunology, and cancer. Inbred mice exhibit identical intra-strain genetics and divergent inter-strain phenotypes. The cognitive and behavioral divergences must be controlled by the variances of structure and function of their brains; however, the underlying morphological features of strain-to-strain difference remain obscure. Here, in vivo microscopic magnetic resonance imaging was optimized to image the mouse brains by using an isotropic resolution of 80 μm. Next, in vivo templates were created from the data from four major inbred mouse strains (C57Bl/6, BALB/cBy, C3H/He, and DBA/2). A strain-mixed brain template was also created, and the template was then employed to establish automatic voxel-based morphometry (VBM) for the mouse brain. The VBM assessment revealed strain-specific brain morphologies concerning the gray matter volume of the four strains, with a smaller volume in the primary visual cortex for the C3H/He strain, and a smaller volume in the primary auditory cortex and field CA1 of the hippocampus for the DBA/2 strain. These findings would contribute to the basis of for understanding morphological phenotype of the inbred mouse strain and may indicate a relationship between brain morphology and strain-specific cognition and behavior.Keigo HikishimaYuji KomakiFumiko SekiYasuyuki OhnishiHirotaka J. OkanoHideyuki OkanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Keigo Hikishima Yuji Komaki Fumiko Seki Yasuyuki Ohnishi Hirotaka J. Okano Hideyuki Okano In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain
description	Abstract Hundreds of inbred mouse strains are established for use in a broad spectrum of basic research fields, including genetics, neuroscience, immunology, and cancer. Inbred mice exhibit identical intra-strain genetics and divergent inter-strain phenotypes. The cognitive and behavioral divergences must be controlled by the variances of structure and function of their brains; however, the underlying morphological features of strain-to-strain difference remain obscure. Here, in vivo microscopic magnetic resonance imaging was optimized to image the mouse brains by using an isotropic resolution of 80 μm. Next, in vivo templates were created from the data from four major inbred mouse strains (C57Bl/6, BALB/cBy, C3H/He, and DBA/2). A strain-mixed brain template was also created, and the template was then employed to establish automatic voxel-based morphometry (VBM) for the mouse brain. The VBM assessment revealed strain-specific brain morphologies concerning the gray matter volume of the four strains, with a smaller volume in the primary visual cortex for the C3H/He strain, and a smaller volume in the primary auditory cortex and field CA1 of the hippocampus for the DBA/2 strain. These findings would contribute to the basis of for understanding morphological phenotype of the inbred mouse strain and may indicate a relationship between brain morphology and strain-specific cognition and behavior.
format	article
author	Keigo Hikishima Yuji Komaki Fumiko Seki Yasuyuki Ohnishi Hirotaka J. Okano Hideyuki Okano
author_facet	Keigo Hikishima Yuji Komaki Fumiko Seki Yasuyuki Ohnishi Hirotaka J. Okano Hideyuki Okano
author_sort	Keigo Hikishima
title	In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain
title_short	In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain
title_full	In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain
title_fullStr	In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain
title_full_unstemmed	In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain
title_sort	in vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f932a284d4ac4b6bb78f5603aa7892aa
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In vivo microscopic voxel-based morphometry with a brain template to characterize strain-specific structures in the mouse brain

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