Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior

Abstract Mammalian cells have been widely shown to respond to nano- and microtopography that mimics the extracellular matrix. Synthetic nano- and micron-sized structures are therefore of great interest in the field of tissue engineering, where polymers are particularly attractive due to excellent bi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nina Buch-Månson, Arnaud Spangenberg, Laura Piedad Chia Gomez, Jean-Pierre Malval, Olivier Soppera, Karen L. Martinez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f93f07564d9d4e21b3902e5c87108317
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f93f07564d9d4e21b3902e5c87108317
record_format	dspace
spelling	oai:doaj.org-article:f93f07564d9d4e21b3902e5c871083172021-12-02T15:05:52ZRapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior10.1038/s41598-017-09208-y2045-2322https://doaj.org/article/f93f07564d9d4e21b3902e5c871083172017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09208-yhttps://doaj.org/toc/2045-2322Abstract Mammalian cells have been widely shown to respond to nano- and microtopography that mimics the extracellular matrix. Synthetic nano- and micron-sized structures are therefore of great interest in the field of tissue engineering, where polymers are particularly attractive due to excellent biocompatibility and versatile fabrication methods. Ordered arrays of polymeric pillars provide a controlled topographical environment to study and manipulate cells, but processing methods are typically either optimized for the nano- or microscale. Here, we demonstrate polymeric nanopillar (NP) fabrication using 3D direct laser writing (3D DLW), which offers a rapid prototyping across both size regimes. The NPs are interfaced with NIH3T3 cells and the effect of tuning geometrical parameters of the NP array is investigated. Cells are found to adhere on a wide range of geometries, but the interface depends on NP density and length. The Cell Interface with Nanostructure Arrays (CINA) model is successfully extended to predict the type of interface formed on different NP geometries, which is found to correlate with the efficiency of cell alignment along the NPs. The combination of the CINA model with the highly versatile 3D DLW fabrication thus holds the promise of improved design of polymeric NP arrays for controlling cell growth.Nina Buch-MånsonArnaud SpangenbergLaura Piedad Chia GomezJean-Pierre MalvalOlivier SopperaKaren L. MartinezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nina Buch-Månson Arnaud Spangenberg Laura Piedad Chia Gomez Jean-Pierre Malval Olivier Soppera Karen L. Martinez Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior
description	Abstract Mammalian cells have been widely shown to respond to nano- and microtopography that mimics the extracellular matrix. Synthetic nano- and micron-sized structures are therefore of great interest in the field of tissue engineering, where polymers are particularly attractive due to excellent biocompatibility and versatile fabrication methods. Ordered arrays of polymeric pillars provide a controlled topographical environment to study and manipulate cells, but processing methods are typically either optimized for the nano- or microscale. Here, we demonstrate polymeric nanopillar (NP) fabrication using 3D direct laser writing (3D DLW), which offers a rapid prototyping across both size regimes. The NPs are interfaced with NIH3T3 cells and the effect of tuning geometrical parameters of the NP array is investigated. Cells are found to adhere on a wide range of geometries, but the interface depends on NP density and length. The Cell Interface with Nanostructure Arrays (CINA) model is successfully extended to predict the type of interface formed on different NP geometries, which is found to correlate with the efficiency of cell alignment along the NPs. The combination of the CINA model with the highly versatile 3D DLW fabrication thus holds the promise of improved design of polymeric NP arrays for controlling cell growth.
format	article
author	Nina Buch-Månson Arnaud Spangenberg Laura Piedad Chia Gomez Jean-Pierre Malval Olivier Soppera Karen L. Martinez
author_facet	Nina Buch-Månson Arnaud Spangenberg Laura Piedad Chia Gomez Jean-Pierre Malval Olivier Soppera Karen L. Martinez
author_sort	Nina Buch-Månson
title	Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior
title_short	Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior
title_full	Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior
title_fullStr	Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior
title_full_unstemmed	Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior
title_sort	rapid prototyping of polymeric nanopillars by 3d direct laser writing for controlling cell behavior
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f93f07564d9d4e21b3902e5c87108317
work_keys_str_mv	AT ninabuchmanson rapidprototypingofpolymericnanopillarsby3ddirectlaserwritingforcontrollingcellbehavior AT arnaudspangenberg rapidprototypingofpolymericnanopillarsby3ddirectlaserwritingforcontrollingcellbehavior AT laurapiedadchiagomez rapidprototypingofpolymericnanopillarsby3ddirectlaserwritingforcontrollingcellbehavior AT jeanpierremalval rapidprototypingofpolymericnanopillarsby3ddirectlaserwritingforcontrollingcellbehavior AT oliviersoppera rapidprototypingofpolymericnanopillarsby3ddirectlaserwritingforcontrollingcellbehavior AT karenlmartinez rapidprototypingofpolymericnanopillarsby3ddirectlaserwritingforcontrollingcellbehavior
_version_	1718388682372677632

Rapid Prototyping of Polymeric Nanopillars by 3D Direct Laser Writing for Controlling Cell Behavior

Ejemplares similares