Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021

We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by rev...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dong-Hun Lee, Zeinab H. Helal, Junwon Kim, Amelia Hunt, Alyza Barbieri, Natalie Tocco, Salvatore Frasca, Kirklyn Kerr, Ji-Yeon Hyeon, David H. Chung, Guillermo Risatti
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	SARS-CoV-2 COVID-19 dog sudden death Connecticut epidemiology Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/f959b8dbeabd47ef82e6d0f83e392f10
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f959b8dbeabd47ef82e6d0f83e392f10
record_format	dspace
spelling	oai:doaj.org-article:f959b8dbeabd47ef82e6d0f83e392f102021-11-25T19:12:39ZSevere Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 202110.3390/v131121411999-4915https://doaj.org/article/f959b8dbeabd47ef82e6d0f83e392f102021-10-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2141https://doaj.org/toc/1999-4915We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by reverse transcription real time-PCR (RT-qPCR). Negative and positive sense strands of viral RNA were visualized by in situ hybridization using RNAscope technology. Complete genome sequencing and phylogenetic analysis of the hCoV-19/USA/CT-CVMDL-Dog-1/2021 (CT_Dog/2021) virus were conducted to identify the origin and lineage of the virus. The CT_Dog/2021 virus belonged to the GH/B1.2. genetic lineage and was genetically similar to SARS-CoV-2 identified in humans in the U.S. during the winter of 2020–2021. However, it was not related to other SARS-CoV-2 variants identified from companion animals in the U.S. It contained both the D614G in spike and P323L in nsp12 substitutions, which have become the dominant mutations in the United States. The continued sporadic detections of SARS-CoV-2 in companion animals warrant public health concerns about the zoonotic potential of SARS-CoV-2 and enhance our collective understanding of the epidemiology of the virus.Dong-Hun LeeZeinab H. HelalJunwon KimAmelia HuntAlyza BarbieriNatalie ToccoSalvatore FrascaKirklyn KerrJi-Yeon HyeonDavid H. ChungGuillermo RisattiMDPI AGarticleSARS-CoV-2COVID-19dogsudden deathConnecticutepidemiologyMicrobiologyQR1-502ENViruses, Vol 13, Iss 2141, p 2141 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	SARS-CoV-2 COVID-19 dog sudden death Connecticut epidemiology Microbiology QR1-502
spellingShingle	SARS-CoV-2 COVID-19 dog sudden death Connecticut epidemiology Microbiology QR1-502 Dong-Hun Lee Zeinab H. Helal Junwon Kim Amelia Hunt Alyza Barbieri Natalie Tocco Salvatore Frasca Kirklyn Kerr Ji-Yeon Hyeon David H. Chung Guillermo Risatti Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
description	We report the first detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a 3-month-old dog in Connecticut that died suddenly and was submitted to the state veterinary diagnostic laboratory for postmortem examination. Viral RNA was detected in multiple organs of the dog by reverse transcription real time-PCR (RT-qPCR). Negative and positive sense strands of viral RNA were visualized by in situ hybridization using RNAscope technology. Complete genome sequencing and phylogenetic analysis of the hCoV-19/USA/CT-CVMDL-Dog-1/2021 (CT_Dog/2021) virus were conducted to identify the origin and lineage of the virus. The CT_Dog/2021 virus belonged to the GH/B1.2. genetic lineage and was genetically similar to SARS-CoV-2 identified in humans in the U.S. during the winter of 2020–2021. However, it was not related to other SARS-CoV-2 variants identified from companion animals in the U.S. It contained both the D614G in spike and P323L in nsp12 substitutions, which have become the dominant mutations in the United States. The continued sporadic detections of SARS-CoV-2 in companion animals warrant public health concerns about the zoonotic potential of SARS-CoV-2 and enhance our collective understanding of the epidemiology of the virus.
format	article
author	Dong-Hun Lee Zeinab H. Helal Junwon Kim Amelia Hunt Alyza Barbieri Natalie Tocco Salvatore Frasca Kirklyn Kerr Ji-Yeon Hyeon David H. Chung Guillermo Risatti
author_facet	Dong-Hun Lee Zeinab H. Helal Junwon Kim Amelia Hunt Alyza Barbieri Natalie Tocco Salvatore Frasca Kirklyn Kerr Ji-Yeon Hyeon David H. Chung Guillermo Risatti
author_sort	Dong-Hun Lee
title	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_short	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_full	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_fullStr	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_full_unstemmed	Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021
title_sort	severe acute respiratory syndrome coronavirus 2 (sars-cov-2) in a dog in connecticut in february 2021
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f959b8dbeabd47ef82e6d0f83e392f10
work_keys_str_mv	AT donghunlee severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT zeinabhhelal severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT junwonkim severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT ameliahunt severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT alyzabarbieri severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT natalietocco severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT salvatorefrasca severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT kirklynkerr severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT jiyeonhyeon severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT davidhchung severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021 AT guillermorisatti severeacuterespiratorysyndromecoronavirus2sarscov2inadoginconnecticutinfebruary2021
_version_	1718410192790487040

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a Dog in Connecticut in February 2021

Ejemplares similares