Sesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>

Hundreds of terpenoids have been isolated from Basidiomycota, among them are volatile mono- and sesquiterpenes with amazing sensory qualities, representing a promising alternative to essential oils from endangered plant species. Sesquiterpene synthases (STS) appear to be an abundant class of enzymes...

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Autores principales:	Nils Püth, Franziska Ersoy, Ulrich Krings, Ralf G. Berger
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	sesquiterpenes terpene cyclases metal ion dependency phylogenetic analysis heterologous expression <i>Cerrena unicolor</i> Chemical technology TP1-1185 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/f9608c85975749c98bb8437b071dde80
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spelling	oai:doaj.org-article:f9608c85975749c98bb8437b071dde802021-11-25T17:06:20ZSesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>10.3390/catal111113612073-4344https://doaj.org/article/f9608c85975749c98bb8437b071dde802021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4344/11/11/1361https://doaj.org/toc/2073-4344Hundreds of terpenoids have been isolated from Basidiomycota, among them are volatile mono- and sesquiterpenes with amazing sensory qualities, representing a promising alternative to essential oils from endangered plant species. Sesquiterpene synthases (STS) appear to be an abundant class of enzymes in these fungi. The basidiomycete <i>Cerrena unicolor</i>, a known sesquiterpene producer, was in silico screened for sesquiterpene cyclases via homology Basic Local Alignment Search Tool searches. Cyclase genes identified were cloned and heterologously expressed in <i>Escherichia coli</i> Bl21 using pCOLD I as the expression vector. Ten cyclases were successfully produced and purified, and their identity was confirmed using amino acid sequencing of tryptic peptides by nano-liquid chromatography-high resolution-electrospray ionization-tandem mass spectrometry. Gas chromatography/mass spectrometry analysis was applied to characterize these cyclases according to the formation of sesquiterpene hydrocarbons and oxidized terpenoids. Bioinformatic characterization and phylogenetic determination allowed for the classification of these diverse fungal enzymes. A representative single and a multi-product STS, respectively, were further analyzed for their dependency from divalent metal cations as a cofactor for the catalytic activity.Nils PüthFranziska ErsoyUlrich KringsRalf G. BergerMDPI AGarticlesesquiterpenesterpene cyclasesmetal ion dependencyphylogenetic analysisheterologous expression<i>Cerrena unicolor</i>Chemical technologyTP1-1185ChemistryQD1-999ENCatalysts, Vol 11, Iss 1361, p 1361 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	sesquiterpenes terpene cyclases metal ion dependency phylogenetic analysis heterologous expression <i>Cerrena unicolor</i> Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	sesquiterpenes terpene cyclases metal ion dependency phylogenetic analysis heterologous expression <i>Cerrena unicolor</i> Chemical technology TP1-1185 Chemistry QD1-999 Nils Püth Franziska Ersoy Ulrich Krings Ralf G. Berger Sesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>
description	Hundreds of terpenoids have been isolated from Basidiomycota, among them are volatile mono- and sesquiterpenes with amazing sensory qualities, representing a promising alternative to essential oils from endangered plant species. Sesquiterpene synthases (STS) appear to be an abundant class of enzymes in these fungi. The basidiomycete <i>Cerrena unicolor</i>, a known sesquiterpene producer, was in silico screened for sesquiterpene cyclases via homology Basic Local Alignment Search Tool searches. Cyclase genes identified were cloned and heterologously expressed in <i>Escherichia coli</i> Bl21 using pCOLD I as the expression vector. Ten cyclases were successfully produced and purified, and their identity was confirmed using amino acid sequencing of tryptic peptides by nano-liquid chromatography-high resolution-electrospray ionization-tandem mass spectrometry. Gas chromatography/mass spectrometry analysis was applied to characterize these cyclases according to the formation of sesquiterpene hydrocarbons and oxidized terpenoids. Bioinformatic characterization and phylogenetic determination allowed for the classification of these diverse fungal enzymes. A representative single and a multi-product STS, respectively, were further analyzed for their dependency from divalent metal cations as a cofactor for the catalytic activity.
format	article
author	Nils Püth Franziska Ersoy Ulrich Krings Ralf G. Berger
author_facet	Nils Püth Franziska Ersoy Ulrich Krings Ralf G. Berger
author_sort	Nils Püth
title	Sesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>
title_short	Sesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>
title_full	Sesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>
title_fullStr	Sesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>
title_full_unstemmed	Sesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>
title_sort	sesquiterpene cyclases from the basidiomycete <i>cerrena unicolor</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f9608c85975749c98bb8437b071dde80
work_keys_str_mv	AT nilsputh sesquiterpenecyclasesfromthebasidiomyceteicerrenaunicolori AT franziskaersoy sesquiterpenecyclasesfromthebasidiomyceteicerrenaunicolori AT ulrichkrings sesquiterpenecyclasesfromthebasidiomyceteicerrenaunicolori AT ralfgberger sesquiterpenecyclasesfromthebasidiomyceteicerrenaunicolori
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Sesquiterpene Cyclases from the Basidiomycete <i>Cerrena unicolor</i>

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