EFFECT OF PREANALYTICAL VARIABLES ON SERUM THYROID STIMULATING HORMONE ANALYSIS BY CHEMILUMINESCENCE METHOD

Objective: To identify effect of pre-analytical variables on serum thyroid stimulating hormone. Study Design: Cross sectional study. Place and Duration of Study: Armed Forces Institute of Pathology (AFIP) Rawalpindi, Department of Chemical Pathology & Endocrinology, from Mar 2018 to Aug 20...

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Autores principales: Ambreen Rehman, Naveed Asif, Saima Shakeel Malik, Waqas Sheikh, Quratulain ., Muhammad Tahir Khadim
Formato: article
Lenguaje:EN
Publicado: Army Medical College Rawalpindi 2021
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Acceso en línea:https://doi.org/10.51253/pafmj.v71iSuppl-1.6202
https://doaj.org/article/f96876c992e446c4a972e1cda852a723
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spelling oai:doaj.org-article:f96876c992e446c4a972e1cda852a7232021-12-02T17:18:51ZEFFECT OF PREANALYTICAL VARIABLES ON SERUM THYROID STIMULATING HORMONE ANALYSIS BY CHEMILUMINESCENCE METHODhttps://doi.org/10.51253/pafmj.v71iSuppl-1.62020030-96482411-8842https://doaj.org/article/f96876c992e446c4a972e1cda852a7232021-01-01T00:00:00Zhttps://pafmj.org/index.php/PAFMJ/article/view/6202https://doaj.org/toc/0030-9648https://doaj.org/toc/2411-8842Objective: To identify effect of pre-analytical variables on serum thyroid stimulating hormone. Study Design: Cross sectional study. Place and Duration of Study: Armed Forces Institute of Pathology (AFIP) Rawalpindi, Department of Chemical Pathology & Endocrinology, from Mar 2018 to Aug 2018. Methodology: Hundred subjects with ages ranging from 18 to 34 years, irrespective of gender, were randomly selected for this study. Five milliliters venous blood sample was collected from each subject in a serum separator and divided into two aliquots. First aliquot was centrifuged and analyzed immediately for TSH, while second aliquot was stored for 24 hours and was then analyzed. TSH was measured by third generation assay using chemiluminescence technique on ADVIA Centaur® XP. Serum TSH levels were also analyzed twice daily; in the morning (0800 to 0900 hours) and afternoon (1400 to 1600 hours). Data was analyzed using SPSS version 24. Frequency and percentages were calculated for qualitative variables like gender and pre-analytical variables. Test of significance Mann-Whitney U-test was applied and p-value <0.05 was taken as significant. Results: Mean age of subjects was 23 ± 3.4 years. Change in circadian rhythm was observed in 17 (28%) males and 14 (36%) females. Statistically significant association was found between morning and evening TSH levels, while no change was observed in TSH level by early and late centrifugation of samples. Conclusion: TSH levels vary significantly between blood samples collected at different timings of the day from the same person. TSH is resistant to degradation, immunologically stable, and reasonably insensitive to potential problems associated with routine specimen handling, when measured by immunoassay technique. Therefore, it is helpful in large epidemiological studies and small size laboratory, which require long transportation time and storage.Ambreen RehmanNaveed AsifSaima Shakeel MalikWaqas SheikhQuratulain .Muhammad Tahir KhadimArmy Medical College Rawalpindiarticlecentrifugationpreanalytical variablesthyroid stimulating hormoneMedicineRMedicine (General)R5-920ENPakistan Armed Forces Medical Journal, Vol 71, Iss SUPPL-1, Pp 102-105 (2021)
institution DOAJ
collection DOAJ
language EN
topic centrifugation
preanalytical variables
thyroid stimulating hormone
Medicine
R
Medicine (General)
R5-920
spellingShingle centrifugation
preanalytical variables
thyroid stimulating hormone
Medicine
R
Medicine (General)
R5-920
Ambreen Rehman
Naveed Asif
Saima Shakeel Malik
Waqas Sheikh
Quratulain .
Muhammad Tahir Khadim
EFFECT OF PREANALYTICAL VARIABLES ON SERUM THYROID STIMULATING HORMONE ANALYSIS BY CHEMILUMINESCENCE METHOD
description Objective: To identify effect of pre-analytical variables on serum thyroid stimulating hormone. Study Design: Cross sectional study. Place and Duration of Study: Armed Forces Institute of Pathology (AFIP) Rawalpindi, Department of Chemical Pathology & Endocrinology, from Mar 2018 to Aug 2018. Methodology: Hundred subjects with ages ranging from 18 to 34 years, irrespective of gender, were randomly selected for this study. Five milliliters venous blood sample was collected from each subject in a serum separator and divided into two aliquots. First aliquot was centrifuged and analyzed immediately for TSH, while second aliquot was stored for 24 hours and was then analyzed. TSH was measured by third generation assay using chemiluminescence technique on ADVIA Centaur® XP. Serum TSH levels were also analyzed twice daily; in the morning (0800 to 0900 hours) and afternoon (1400 to 1600 hours). Data was analyzed using SPSS version 24. Frequency and percentages were calculated for qualitative variables like gender and pre-analytical variables. Test of significance Mann-Whitney U-test was applied and p-value <0.05 was taken as significant. Results: Mean age of subjects was 23 ± 3.4 years. Change in circadian rhythm was observed in 17 (28%) males and 14 (36%) females. Statistically significant association was found between morning and evening TSH levels, while no change was observed in TSH level by early and late centrifugation of samples. Conclusion: TSH levels vary significantly between blood samples collected at different timings of the day from the same person. TSH is resistant to degradation, immunologically stable, and reasonably insensitive to potential problems associated with routine specimen handling, when measured by immunoassay technique. Therefore, it is helpful in large epidemiological studies and small size laboratory, which require long transportation time and storage.
format article
author Ambreen Rehman
Naveed Asif
Saima Shakeel Malik
Waqas Sheikh
Quratulain .
Muhammad Tahir Khadim
author_facet Ambreen Rehman
Naveed Asif
Saima Shakeel Malik
Waqas Sheikh
Quratulain .
Muhammad Tahir Khadim
author_sort Ambreen Rehman
title EFFECT OF PREANALYTICAL VARIABLES ON SERUM THYROID STIMULATING HORMONE ANALYSIS BY CHEMILUMINESCENCE METHOD
title_short EFFECT OF PREANALYTICAL VARIABLES ON SERUM THYROID STIMULATING HORMONE ANALYSIS BY CHEMILUMINESCENCE METHOD
title_full EFFECT OF PREANALYTICAL VARIABLES ON SERUM THYROID STIMULATING HORMONE ANALYSIS BY CHEMILUMINESCENCE METHOD
title_fullStr EFFECT OF PREANALYTICAL VARIABLES ON SERUM THYROID STIMULATING HORMONE ANALYSIS BY CHEMILUMINESCENCE METHOD
title_full_unstemmed EFFECT OF PREANALYTICAL VARIABLES ON SERUM THYROID STIMULATING HORMONE ANALYSIS BY CHEMILUMINESCENCE METHOD
title_sort effect of preanalytical variables on serum thyroid stimulating hormone analysis by chemiluminescence method
publisher Army Medical College Rawalpindi
publishDate 2021
url https://doi.org/10.51253/pafmj.v71iSuppl-1.6202
https://doaj.org/article/f96876c992e446c4a972e1cda852a723
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