Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences

Abstract Background Comparing the microbiome compositions obtained under different physiological conditions has frequently been attempted in recent years to understand the functional influence of microbiomes in the occurrence of various human diseases. Methods In the present work, we analyzed 102 mi...

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Autores principales:	Sukjung Choi, Jongsuk Chung, Mi-La Cho, Donghyun Park, Sun Shim Choi
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Colorectal cancer Microbiome 16S rRNA F. nucleatum B. fragilis Medicine R
Acceso en línea:	https://doaj.org/article/f975ad3ee20349f2a818142e74a6d1d5
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spelling	oai:doaj.org-article:f975ad3ee20349f2a818142e74a6d1d52021-12-05T12:06:01ZAnalysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences10.1186/s12967-021-03154-01479-5876https://doaj.org/article/f975ad3ee20349f2a818142e74a6d1d52021-11-01T00:00:00Zhttps://doi.org/10.1186/s12967-021-03154-0https://doaj.org/toc/1479-5876Abstract Background Comparing the microbiome compositions obtained under different physiological conditions has frequently been attempted in recent years to understand the functional influence of microbiomes in the occurrence of various human diseases. Methods In the present work, we analyzed 102 microbiome datasets containing tumor- and normal tissue-derived microbiomes obtained from a total of 51 Korean colorectal cancer (CRC) patients using 16S rRNA amplicon sequencing. Two types of comparisons were used: ‘normal versus (vs.) tumor’ comparison and ‘recurrent vs. nonrecurrent’ comparison, for which the prognosis of patients was retrospectively determined. Results As a result, we observed that in the ‘normal vs. tumor’ comparison, three phyla, Firmicutes, Actinobacteria, and Bacteroidetes, were more abundant in normal tissues, whereas some pathogenic bacteria, including Fusobacterium nucleatum and Bacteroides fragilis, were more abundant in tumor tissues. We also found that bacteria with metabolic pathways related to the production of bacterial motility proteins or bile acid secretion were more enriched in tumor tissues. In addition, the amount of these two pathogenic bacteria was positively correlated with the expression levels of host genes involved in the cell cycle and cell proliferation, confirming the association of microbiomes with tumorigenic pathway genes in the host. Surprisingly, in the ‘recurrent vs. nonrecurrent’ comparison, we observed that these two pathogenic bacteria were more abundant in the patients without recurrence than in the patients with recurrence. The same conclusion was drawn in the analysis of both normal and tumor-derived microbiomes. Conclusions Taken together, it seems that understanding the composition of tissue microbiomes is useful for predicting the prognosis of CRC patients.Sukjung ChoiJongsuk ChungMi-La ChoDonghyun ParkSun Shim ChoiBMCarticleColorectal cancerMicrobiome16S rRNAF. nucleatumB. fragilisMedicineRENJournal of Translational Medicine, Vol 19, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Colorectal cancer Microbiome 16S rRNA F. nucleatum B. fragilis Medicine R
spellingShingle	Colorectal cancer Microbiome 16S rRNA F. nucleatum B. fragilis Medicine R Sukjung Choi Jongsuk Chung Mi-La Cho Donghyun Park Sun Shim Choi Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences
description	Abstract Background Comparing the microbiome compositions obtained under different physiological conditions has frequently been attempted in recent years to understand the functional influence of microbiomes in the occurrence of various human diseases. Methods In the present work, we analyzed 102 microbiome datasets containing tumor- and normal tissue-derived microbiomes obtained from a total of 51 Korean colorectal cancer (CRC) patients using 16S rRNA amplicon sequencing. Two types of comparisons were used: ‘normal versus (vs.) tumor’ comparison and ‘recurrent vs. nonrecurrent’ comparison, for which the prognosis of patients was retrospectively determined. Results As a result, we observed that in the ‘normal vs. tumor’ comparison, three phyla, Firmicutes, Actinobacteria, and Bacteroidetes, were more abundant in normal tissues, whereas some pathogenic bacteria, including Fusobacterium nucleatum and Bacteroides fragilis, were more abundant in tumor tissues. We also found that bacteria with metabolic pathways related to the production of bacterial motility proteins or bile acid secretion were more enriched in tumor tissues. In addition, the amount of these two pathogenic bacteria was positively correlated with the expression levels of host genes involved in the cell cycle and cell proliferation, confirming the association of microbiomes with tumorigenic pathway genes in the host. Surprisingly, in the ‘recurrent vs. nonrecurrent’ comparison, we observed that these two pathogenic bacteria were more abundant in the patients without recurrence than in the patients with recurrence. The same conclusion was drawn in the analysis of both normal and tumor-derived microbiomes. Conclusions Taken together, it seems that understanding the composition of tissue microbiomes is useful for predicting the prognosis of CRC patients.
format	article
author	Sukjung Choi Jongsuk Chung Mi-La Cho Donghyun Park Sun Shim Choi
author_facet	Sukjung Choi Jongsuk Chung Mi-La Cho Donghyun Park Sun Shim Choi
author_sort	Sukjung Choi
title	Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences
title_short	Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences
title_full	Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences
title_fullStr	Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences
title_full_unstemmed	Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences
title_sort	analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16s rrna sequences
publisher	BMC
publishDate	2021
url	https://doaj.org/article/f975ad3ee20349f2a818142e74a6d1d5
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Analysis of changes in microbiome compositions related to the prognosis of colorectal cancer patients based on tissue-derived 16S rRNA sequences

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