Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia

The present study assessed the protective aspects of the dietary exposure to black cumin (Nigella sativa, NS) powder on immune response, liver and kidney enzymes, tissue antioxidant capacity, gene expression, and testing the antibacterial activity against Burkholderia cepacia (B. cepacia, BC). Nile...

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Autores principales:	Heba H. Mahboub, Hassnaa Mahmoud Elsheshtawy, Nagwa I. Sheraiba, Esra.M. Fahmy, Shimaa R. masoud, Eman A.A. Mohamed, Noha S. Abdelnaeim, Dalia Ibrahim Mohamed, Tamer Ahmed Ismail, Shaimaa A.A. Ahmed
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Black cumin powder Blood Chemistry Burkholderia cepacia Gene expression Nile tilapia Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/f9780cffbcbc445b9b97928be97eb3a5
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spelling	oai:doaj.org-article:f9780cffbcbc445b9b97928be97eb3a52021-11-14T04:34:22ZDietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia2352-513410.1016/j.aqrep.2021.100943https://doaj.org/article/f9780cffbcbc445b9b97928be97eb3a52022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352513421003604https://doaj.org/toc/2352-5134The present study assessed the protective aspects of the dietary exposure to black cumin (Nigella sativa, NS) powder on immune response, liver and kidney enzymes, tissue antioxidant capacity, gene expression, and testing the antibacterial activity against Burkholderia cepacia (B. cepacia, BC). Nile tilapia (O. niloticus) (n = 150) were divided into 5 groups, the first was control (Cont) neither challenged nor treated, and fed a basal diet for 59 days. The second one received 30 g kg−1 of NS-incorporated diet and farmed for 45 days (45NS). The third (Cont-BC) and the fourth (14NS-BC) groups were fed a basal diet, while the fifth (59NS-BC) was fed NS-supplemented diet for 45 days. Then, the last three groups were intraperitoneally inoculated with 0.2 mL of virulent BC, and kept under observation for 14 days. During the observation period, Cont-BC group was continuously received a basal diet, while 14NS-BC and 59NS-BC were fed on NS-supplemented diet. Results showed that fish received NS-dietary supplement exhibited significantly (P < 0.05) elevated levels of myeloperoxidase, lysozyme, nitric oxide, superoxide dismutase, and reduced glutathione. A clear modulation was noticed in the levels of cortisol, Malondialdehyde, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, urea, and creatinine. A clear up-regulation of inflammatory cytokines encoding genes, interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin 1, beta (IL-1β) and immune-related gene, immunoglobulin M (IgM), was evident in NS-enriched groups in comparison to the control. On the contrary, challenged fish with bacterium exhibited a clear decrease in immune and antioxidant (SOD, GSH) biomarkers, while a noticeable increase in hepato-renal parameters, cortisol, and MDA levels, besides gene down-regulation. Thus, we recommend the usage of NS as a nutraceutical to stimulate immunity and as an antibacterial agent in Nile tilapia owing to modulating the immune-antioxidant status, hepato-renal function, and gene expression, besides enhancing the fish survival rates post-exposure to B. cepacia.Heba H. MahboubHassnaa Mahmoud ElsheshtawyNagwa I. SheraibaEsra.M. FahmyShimaa R. masoudEman A.A. MohamedNoha S. AbdelnaeimDalia Ibrahim MohamedTamer Ahmed IsmailShaimaa A.A. AhmedElsevierarticleBlack cumin powderBlood ChemistryBurkholderia cepaciaGene expressionNile tilapiaAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100943- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Black cumin powder Blood Chemistry Burkholderia cepacia Gene expression Nile tilapia Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Black cumin powder Blood Chemistry Burkholderia cepacia Gene expression Nile tilapia Aquaculture. Fisheries. Angling SH1-691 Heba H. Mahboub Hassnaa Mahmoud Elsheshtawy Nagwa I. Sheraiba Esra.M. Fahmy Shimaa R. masoud Eman A.A. Mohamed Noha S. Abdelnaeim Dalia Ibrahim Mohamed Tamer Ahmed Ismail Shaimaa A.A. Ahmed Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia
description	The present study assessed the protective aspects of the dietary exposure to black cumin (Nigella sativa, NS) powder on immune response, liver and kidney enzymes, tissue antioxidant capacity, gene expression, and testing the antibacterial activity against Burkholderia cepacia (B. cepacia, BC). Nile tilapia (O. niloticus) (n = 150) were divided into 5 groups, the first was control (Cont) neither challenged nor treated, and fed a basal diet for 59 days. The second one received 30 g kg−1 of NS-incorporated diet and farmed for 45 days (45NS). The third (Cont-BC) and the fourth (14NS-BC) groups were fed a basal diet, while the fifth (59NS-BC) was fed NS-supplemented diet for 45 days. Then, the last three groups were intraperitoneally inoculated with 0.2 mL of virulent BC, and kept under observation for 14 days. During the observation period, Cont-BC group was continuously received a basal diet, while 14NS-BC and 59NS-BC were fed on NS-supplemented diet. Results showed that fish received NS-dietary supplement exhibited significantly (P < 0.05) elevated levels of myeloperoxidase, lysozyme, nitric oxide, superoxide dismutase, and reduced glutathione. A clear modulation was noticed in the levels of cortisol, Malondialdehyde, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, urea, and creatinine. A clear up-regulation of inflammatory cytokines encoding genes, interleukin 8 (IL-8), interleukin 10 (IL-10), interleukin 1, beta (IL-1β) and immune-related gene, immunoglobulin M (IgM), was evident in NS-enriched groups in comparison to the control. On the contrary, challenged fish with bacterium exhibited a clear decrease in immune and antioxidant (SOD, GSH) biomarkers, while a noticeable increase in hepato-renal parameters, cortisol, and MDA levels, besides gene down-regulation. Thus, we recommend the usage of NS as a nutraceutical to stimulate immunity and as an antibacterial agent in Nile tilapia owing to modulating the immune-antioxidant status, hepato-renal function, and gene expression, besides enhancing the fish survival rates post-exposure to B. cepacia.
format	article
author	Heba H. Mahboub Hassnaa Mahmoud Elsheshtawy Nagwa I. Sheraiba Esra.M. Fahmy Shimaa R. masoud Eman A.A. Mohamed Noha S. Abdelnaeim Dalia Ibrahim Mohamed Tamer Ahmed Ismail Shaimaa A.A. Ahmed
author_facet	Heba H. Mahboub Hassnaa Mahmoud Elsheshtawy Nagwa I. Sheraiba Esra.M. Fahmy Shimaa R. masoud Eman A.A. Mohamed Noha S. Abdelnaeim Dalia Ibrahim Mohamed Tamer Ahmed Ismail Shaimaa A.A. Ahmed
author_sort	Heba H. Mahboub
title	Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia
title_short	Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia
title_full	Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia
title_fullStr	Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia
title_full_unstemmed	Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia
title_sort	dietary black cumin (nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of nile tilapia (oreochromis niloticus) infected by burkholderia cepacia
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/f9780cffbcbc445b9b97928be97eb3a5
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Dietary black cumin (Nigella sativa) improved hemato-biochemical, oxidative stress, gene expression, and immunological response of Nile tilapia (Oreochromis niloticus) infected by Burkholderia cepacia

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