GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.

Streptococcus mutans is a cariogenic pathogen that produces an extracellular polysaccharide (glucan) from dietary sugars, which allows it to establish a reproductive niche and secrete acids that degrade tooth enamel. While two enzymes (GlmS and NagB) are known to be key factors affecting the entranc...

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Autores principales:	Miki Kawada-Matsuo, Yusuke Mazda, Yuichi Oogai, Mikihito Kajiya, Toshihisa Kawai, Sakuo Yamada, Shouichi Miyawaki, Takahiko Oho, Hitoshi Komatsuzawa
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f97d30a20ff24a79ab5bef37172eabf6
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spelling	oai:doaj.org-article:f97d30a20ff24a79ab5bef37172eabf62021-11-18T07:25:00ZGlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.1932-620310.1371/journal.pone.0033382https://doaj.org/article/f97d30a20ff24a79ab5bef37172eabf62012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22438919/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Streptococcus mutans is a cariogenic pathogen that produces an extracellular polysaccharide (glucan) from dietary sugars, which allows it to establish a reproductive niche and secrete acids that degrade tooth enamel. While two enzymes (GlmS and NagB) are known to be key factors affecting the entrance of amino sugars into glycolysis and cell wall synthesis in several other bacteria, their roles in S. mutans remain unclear. Therefore, we investigated the roles of GlmS and NagB in S. mutans sugar metabolism and determined whether they have an effect on virulence. NagB expression increased in the presence of GlcNAc while GlmS expression decreased, suggesting that the regulation of these enzymes, which functionally oppose one another, is dependent on the concentration of environmental GlcNAc. A glmS-inactivated mutant could not grow in the absence of GlcNAc, while nagB-inactivated mutant growth was decreased in the presence of GlcNAc. Also, nagB inactivation was found to decrease the expression of virulence factors, including cell-surface protein antigen and glucosyltransferase, and to decrease biofilm formation and saliva-induced S. mutans aggregation, while glmS inactivation had the opposite effects on virulence factor expression and bacterial aggregation. Our results suggest that GlmS and NagB function in sugar metabolism in opposing directions, increasing and decreasing S. mutans virulence, respectively.Miki Kawada-MatsuoYusuke MazdaYuichi OogaiMikihito KajiyaToshihisa KawaiSakuo YamadaShouichi MiyawakiTakahiko OhoHitoshi KomatsuzawaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e33382 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Miki Kawada-Matsuo Yusuke Mazda Yuichi Oogai Mikihito Kajiya Toshihisa Kawai Sakuo Yamada Shouichi Miyawaki Takahiko Oho Hitoshi Komatsuzawa GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.
description	Streptococcus mutans is a cariogenic pathogen that produces an extracellular polysaccharide (glucan) from dietary sugars, which allows it to establish a reproductive niche and secrete acids that degrade tooth enamel. While two enzymes (GlmS and NagB) are known to be key factors affecting the entrance of amino sugars into glycolysis and cell wall synthesis in several other bacteria, their roles in S. mutans remain unclear. Therefore, we investigated the roles of GlmS and NagB in S. mutans sugar metabolism and determined whether they have an effect on virulence. NagB expression increased in the presence of GlcNAc while GlmS expression decreased, suggesting that the regulation of these enzymes, which functionally oppose one another, is dependent on the concentration of environmental GlcNAc. A glmS-inactivated mutant could not grow in the absence of GlcNAc, while nagB-inactivated mutant growth was decreased in the presence of GlcNAc. Also, nagB inactivation was found to decrease the expression of virulence factors, including cell-surface protein antigen and glucosyltransferase, and to decrease biofilm formation and saliva-induced S. mutans aggregation, while glmS inactivation had the opposite effects on virulence factor expression and bacterial aggregation. Our results suggest that GlmS and NagB function in sugar metabolism in opposing directions, increasing and decreasing S. mutans virulence, respectively.
format	article
author	Miki Kawada-Matsuo Yusuke Mazda Yuichi Oogai Mikihito Kajiya Toshihisa Kawai Sakuo Yamada Shouichi Miyawaki Takahiko Oho Hitoshi Komatsuzawa
author_facet	Miki Kawada-Matsuo Yusuke Mazda Yuichi Oogai Mikihito Kajiya Toshihisa Kawai Sakuo Yamada Shouichi Miyawaki Takahiko Oho Hitoshi Komatsuzawa
author_sort	Miki Kawada-Matsuo
title	GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.
title_short	GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.
title_full	GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.
title_fullStr	GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.
title_full_unstemmed	GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.
title_sort	glms and nagb regulate amino sugar metabolism in opposing directions and affect streptococcus mutans virulence.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/f97d30a20ff24a79ab5bef37172eabf6
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GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence.

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