Addendum: Precise tuning of gene expression levels in mammalian cells

Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constru...

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Autores principales:	Yale S. Michaels, Mike B. Barnkob, Hector Barbosa, Toni A. Baeumler, Mary K. Thompson, Violaine Andre, Huw Colin-York, Marco Fritzsche, Uzi Gileadi, Hilary M. Sheppard, David J. H. F. Knapp, Thomas A. Milne, Vincenzo Cerundolo, Tudor A. Fulga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f982718f87894f9b9dd74cb5a98758c7
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spelling	oai:doaj.org-article:f982718f87894f9b9dd74cb5a98758c72021-12-02T15:36:20ZAddendum: Precise tuning of gene expression levels in mammalian cells10.1038/s41467-019-10615-02041-1723https://doaj.org/article/f982718f87894f9b9dd74cb5a98758c72019-06-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-10615-0https://doaj.org/toc/2041-1723Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Figs. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that step-wise control over gene expression levels using the miSFIT constructs remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented in the accompanying Addendum.Yale S. MichaelsMike B. BarnkobHector BarbosaToni A. BaeumlerMary K. ThompsonViolaine AndreHuw Colin-YorkMarco FritzscheUzi GileadiHilary M. SheppardDavid J. H. F. KnappThomas A. MilneVincenzo CerundoloTudor A. FulgaNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-3 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Yale S. Michaels Mike B. Barnkob Hector Barbosa Toni A. Baeumler Mary K. Thompson Violaine Andre Huw Colin-York Marco Fritzsche Uzi Gileadi Hilary M. Sheppard David J. H. F. Knapp Thomas A. Milne Vincenzo Cerundolo Tudor A. Fulga Addendum: Precise tuning of gene expression levels in mammalian cells
description	Following re-sequencing of the miSFIT constructs used in the paper, two of the construct variants inserted into the 3’UTR of PD-1, namely ‘12C’ and ‘17A, 18G’, have been found to contain additional insertions not present in the other construct variants. The data points corresponding to these constructs in Figs. 2c, f and Supplementary Fig. 9 are therefore no longer valid. However the overall conclusion that step-wise control over gene expression levels using the miSFIT constructs remains unaffected by these errors. Updated versions of Fig. 2 and Supplementary Fig. 9 are presented in the accompanying Addendum.
format	article
author	Yale S. Michaels Mike B. Barnkob Hector Barbosa Toni A. Baeumler Mary K. Thompson Violaine Andre Huw Colin-York Marco Fritzsche Uzi Gileadi Hilary M. Sheppard David J. H. F. Knapp Thomas A. Milne Vincenzo Cerundolo Tudor A. Fulga
author_facet	Yale S. Michaels Mike B. Barnkob Hector Barbosa Toni A. Baeumler Mary K. Thompson Violaine Andre Huw Colin-York Marco Fritzsche Uzi Gileadi Hilary M. Sheppard David J. H. F. Knapp Thomas A. Milne Vincenzo Cerundolo Tudor A. Fulga
author_sort	Yale S. Michaels
title	Addendum: Precise tuning of gene expression levels in mammalian cells
title_short	Addendum: Precise tuning of gene expression levels in mammalian cells
title_full	Addendum: Precise tuning of gene expression levels in mammalian cells
title_fullStr	Addendum: Precise tuning of gene expression levels in mammalian cells
title_full_unstemmed	Addendum: Precise tuning of gene expression levels in mammalian cells
title_sort	addendum: precise tuning of gene expression levels in mammalian cells
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/f982718f87894f9b9dd74cb5a98758c7
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Addendum: Precise tuning of gene expression levels in mammalian cells

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