Regulation of viral gene expression by duck enteritis virus UL54

Abstract Duck enteritis virus (DEV) UL54 is a homologue of human herpes simplex virus-1 (HSV-1) ICP27, which plays essential regulatory roles during infection. Our previous studies indicated that DEV UL54 is an immediate-early protein that can shuttle between the nucleus and the cytoplasm. In the pr...

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Autores principales:	Chaoyue Liu, Anchun Cheng, Mingshu Wang, Shun Chen, Renyong Jia, Dekang Zhu, Mafeng Liu, Kunfeng Sun, Qiao Yang, Ying Wu, Xinxin Zhao, Xiaoyue Chen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/f98c6e48dfa143f090ac910e660a0ec0
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spelling	oai:doaj.org-article:f98c6e48dfa143f090ac910e660a0ec02021-12-02T15:05:00ZRegulation of viral gene expression by duck enteritis virus UL5410.1038/s41598-017-01161-02045-2322https://doaj.org/article/f98c6e48dfa143f090ac910e660a0ec02017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01161-0https://doaj.org/toc/2045-2322Abstract Duck enteritis virus (DEV) UL54 is a homologue of human herpes simplex virus-1 (HSV-1) ICP27, which plays essential regulatory roles during infection. Our previous studies indicated that DEV UL54 is an immediate-early protein that can shuttle between the nucleus and the cytoplasm. In the present study, we found that UL54-deleted DEV (DEV-ΔUL54) exhibits growth kinetics, a plaque size and a viral DNA copy number that are significantly different from those of its parent wild-type virus (DEV-LoxP) and the revertant (DEV-ΔUL54 (Revertant)). Relative viral mRNA levels, reflecting gene expression, the transcription phase and the translation stage, are also significantly different between DEV-ΔUL54-infected cells and DEV-LoxP/DEV-ΔUL54 (Revertant)-infected cells. However, the localization pattern of UL30 mRNA is obviously changed in DEV-ΔUL54-infected cells. These findings suggest that DEV UL54 is important for virus growth and may regulate viral gene expression during transcription, mRNA export and translation.Chaoyue LiuAnchun ChengMingshu WangShun ChenRenyong JiaDekang ZhuMafeng LiuKunfeng SunQiao YangYing WuXinxin ZhaoXiaoyue ChenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Chaoyue Liu Anchun Cheng Mingshu Wang Shun Chen Renyong Jia Dekang Zhu Mafeng Liu Kunfeng Sun Qiao Yang Ying Wu Xinxin Zhao Xiaoyue Chen Regulation of viral gene expression by duck enteritis virus UL54
description	Abstract Duck enteritis virus (DEV) UL54 is a homologue of human herpes simplex virus-1 (HSV-1) ICP27, which plays essential regulatory roles during infection. Our previous studies indicated that DEV UL54 is an immediate-early protein that can shuttle between the nucleus and the cytoplasm. In the present study, we found that UL54-deleted DEV (DEV-ΔUL54) exhibits growth kinetics, a plaque size and a viral DNA copy number that are significantly different from those of its parent wild-type virus (DEV-LoxP) and the revertant (DEV-ΔUL54 (Revertant)). Relative viral mRNA levels, reflecting gene expression, the transcription phase and the translation stage, are also significantly different between DEV-ΔUL54-infected cells and DEV-LoxP/DEV-ΔUL54 (Revertant)-infected cells. However, the localization pattern of UL30 mRNA is obviously changed in DEV-ΔUL54-infected cells. These findings suggest that DEV UL54 is important for virus growth and may regulate viral gene expression during transcription, mRNA export and translation.
format	article
author	Chaoyue Liu Anchun Cheng Mingshu Wang Shun Chen Renyong Jia Dekang Zhu Mafeng Liu Kunfeng Sun Qiao Yang Ying Wu Xinxin Zhao Xiaoyue Chen
author_facet	Chaoyue Liu Anchun Cheng Mingshu Wang Shun Chen Renyong Jia Dekang Zhu Mafeng Liu Kunfeng Sun Qiao Yang Ying Wu Xinxin Zhao Xiaoyue Chen
author_sort	Chaoyue Liu
title	Regulation of viral gene expression by duck enteritis virus UL54
title_short	Regulation of viral gene expression by duck enteritis virus UL54
title_full	Regulation of viral gene expression by duck enteritis virus UL54
title_fullStr	Regulation of viral gene expression by duck enteritis virus UL54
title_full_unstemmed	Regulation of viral gene expression by duck enteritis virus UL54
title_sort	regulation of viral gene expression by duck enteritis virus ul54
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f98c6e48dfa143f090ac910e660a0ec0
work_keys_str_mv	AT chaoyueliu regulationofviralgeneexpressionbyduckenteritisvirusul54 AT anchuncheng regulationofviralgeneexpressionbyduckenteritisvirusul54 AT mingshuwang regulationofviralgeneexpressionbyduckenteritisvirusul54 AT shunchen regulationofviralgeneexpressionbyduckenteritisvirusul54 AT renyongjia regulationofviralgeneexpressionbyduckenteritisvirusul54 AT dekangzhu regulationofviralgeneexpressionbyduckenteritisvirusul54 AT mafengliu regulationofviralgeneexpressionbyduckenteritisvirusul54 AT kunfengsun regulationofviralgeneexpressionbyduckenteritisvirusul54 AT qiaoyang regulationofviralgeneexpressionbyduckenteritisvirusul54 AT yingwu regulationofviralgeneexpressionbyduckenteritisvirusul54 AT xinxinzhao regulationofviralgeneexpressionbyduckenteritisvirusul54 AT xiaoyuechen regulationofviralgeneexpressionbyduckenteritisvirusul54
_version_	1718388993873149952

Regulation of viral gene expression by duck enteritis virus UL54

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